RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic

Luo, Lei; Wang, Qianning; Ma, Fengwang

doi:10.1007/s11250-021-02566-w

Cited by 7 publications

(4 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The number of DEGs identified in this experiment is relatively small (20 DEGs). According to the available literature, compared with the breed or environmental factors, dietary interventions usually cause limited changes in genes expression at the transcriptome level of broilers (Luo et al., 2021; Pascual et al., 2020; Zhang et al., 2018). Moreover, in this study, GO enrichment analysis of these DEGs showed that these genes were mainly enriched in negative regulation of TLRs 9 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals potential mechanisms of the effects of dietary Enteromorpha polysaccharides on bursa of Fabricius in broilers

Qiu

Zhang

Guo

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

The present study was conducted to evaluate the effects of dietary supplementation of Enteromorpha polysaccharides (EP) on relative organ weight of broilers, and RNA‐seq technique was used to reveal the potential molecular mechanisms of the positive effects of EP on relative organ weight. A total of 396 1‐day‐old male chicks (Arbor Acres) were randomly assigned to six dietary treatments containing EP at 0 (EP0), 1000 (EP1000), 2500 (EP2500), 4000 (EP4000), 5500 (EP5500), and 7000 (EP7000) mg/kg levels for a 35‐day feeding trial. At the end of feeding trail, six birds (one bird from each replicate cage) were randomly selected from each treatment and then slaughtered for relative organ weight analysis. The results showed that the relative weight of bursa of Fabricius were increased in the EP1000 group (p < 0.05), and then three bursa of Fabricius samples from each group (EP0 and EP1000) were randomly selected for RNA‐seq analysis. The results of RNA‐seq analysis showed that there were 20 differentially expressed genes (DEGs) between EP0 and EP1000 groups, among the DEGs, 6 genes were upregulated and 14 genes were downregulated by EP1000 supplementation (p‐adjust < 0.05). Gene ontology (GO) enrichment analysis suggested that the DEGs were mainly enriched in negative regulation of toll‐like receptor 9 signaling pathway (p‐corrected < 0.05). Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that the DEGs were mainly enriched in phagosome, mitophagy‐animal, Salmonella infection, autophagy‐animal signaling pathways (p‐corrected = 0.081). Taken together, dietary EP supplementation at 1000 mg/kg level promoted the relative weight of bursa of Fabricius may be involved in improving the immune function of broilers. These findings provided a reference for further exploring the specific molecular mechanism of EP that affecting the organ development in broilers.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveals potential mechanisms of the effects of dietary Enteromorpha polysaccharides on bursa of Fabricius in broilers

Qiu

Zhang

Guo

et al. 2021

Veterinary Medicine & Sci

View full text Add to dashboard Cite

show abstract

“…After obtaining the FPKM value of the gene, we directly compared the differential expression of genes between the two samples. According to FDR < 0.05, and absolute fold change ≥ 2 (Luo et al, 2021), DEGs were screened out.…”

Section: Methodsmentioning

confidence: 99%

Comparative transcriptome analysis of mixed tissues of black porgy ( Acanthopagrus schlegelii ) with differing growth rates

Lin

Zhang

Solberg

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

Black porgy (Acanthopagrus schlegelii) belongs to the order Perciformes, the family Sparidae and the genus Acanthopagrus. It is an essential marine aquaculture fish, with an oblong and flat body and holds the characteristics of fast growth, good meat quality, strong disease resistance and strong adaptability to various environments (Zhu & Zhu, 2014). Fish growth traits refer to the growth-related traits such as body weight, body length, head length, body width, body height and tail stalk length of individual fish. The faster the fish grow, the less time is needed for breeding until market size, the lower the economic cost and the higher the yield at harvest, which can be beneficial for meeting market demands, and increase the economic and ecological benefits of fish breeding (Yin et al., 2020). Therefore, growth traits are not only one of the economic traits that best reflects the growth status of the fish, but it is also a very important reference indicator for the development of fish farming. With the development of aquaculture, the research on growth traits has received more and more attention.

show abstract

“…Transcriptome sequencing technology (RNA-Seq) can accurately and efficiently obtain almost all the transcripts of a specific tissue in a certain period of time, and deeply excavate the subtle changes in the differential expression of genes in the tissue or cell (11). Therefore, the RNA-Seq technique is of great significance for exploring gene expression and regulation mechanisms at transcription level and is widely used in animal nutrition research (12,13).…”

Section: Introductionmentioning

confidence: 99%

Production performance, egg quality, and uterine gene expression for layers as affected by N-Carbamylglutamate supplementation

Wang

2023

Front. Vet. Sci.

View full text Add to dashboard Cite

IntroductionSupplementation of exogenous additives is a strategy to improve laying performance of layers by regulating uterine function. N-Carbamylglutamate (NCG) as an activator for endogenous arginine synthesis has the potential to regulate the laying performance of layers, but its effects have not been fully understood.MethodsThis study investigated the effects of dietary supplementation of NCG on production performance, egg quality, and uterine gene expression in layers. A total of 360 45-week-old layers with a genetic line of Jinghong No. 1 were used in this study. The experimental period was 14 weeks. All birds were divided into 4 treatments with 6 replicates per treatment and 15 birds per replicate. Dietary treatments were based on a basal diet and supplemented with 0, 0.08, 0.12, or 0.16% NCG to form C, N1, N2, and N3 groups.Results and discussionWe found that layers in group N1 had higher egg production rate than those in group C. Egg weight was significantly reduced, while eggshell thickness was significantly improved, by treatment. However, the albumen height and Haugh unit were the lowest in group N3. Based on the above results, groups C and N1 were selected for further transcriptomics analysis of uterine tissue by RNA-seq. More than 7.4 Gb clean reads and 19,882 tentative genes were obtained using the Gallus gallus genome as a reference. Transcriptomics analysis in uterus tissue revealed that 95 differentially expressed genes (DEGs) were upregulated and 127 DEGs were downregulated. Functional annotation and pathway enrichment analysis showed that DEGs in uterine tissue were mainly enriched in glutathione metabolism, cholesterol metabolism, and glycerolipid metabolism, etc. Vitamin A metabolism-related gene, RBP1, nutrient transport-related gene, ALB, protein synthesis-related gene, METTL21C, and calcium transport-related gene, RYR2, CACNB2, RAMP3, and STAC, were significantly regulated by 0.08% NCG supplementation. Therefore, we concluded that NCG supplementation at a dose of 0.08% improved production performance and egg quality of layers by regulating uterus function.

show abstract

RNA-Seq transcriptome analysis of ileum in Taiping chicken supplemented with the dietary probiotic

Cited by 7 publications

References 63 publications

Transcriptome analysis reveals potential mechanisms of the effects of dietary Enteromorpha polysaccharides on bursa of Fabricius in broilers

Transcriptome analysis reveals potential mechanisms of the effects of dietary Enteromorpha polysaccharides on bursa of Fabricius in broilers

Comparative transcriptome analysis of mixed tissues of black porgy ( Acanthopagrus schlegelii ) with differing growth rates

Production performance, egg quality, and uterine gene expression for layers as affected by N-Carbamylglutamate supplementation

Contact Info

Product

Resources

About