2017
DOI: 10.1371/journal.pntd.0006026
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RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation

Abstract: BackgroundLeishmania is a protozoan parasite that alternates its life cycle between the sand-fly vector and the mammalian host. This alternation involves environmental changes and leads the parasite to dynamic modifications in morphology, metabolism, cellular signaling and regulation of gene expression to allow for a rapid adaptation to new conditions. The L-arginine pathway in L. amazonensis is important during the parasite life cycle and interferes in the establishment and maintenance of the infection in mam… Show more

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Cited by 34 publications
(55 citation statements)
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“…In contrast, BALB/c infection with the La-arg − parasite showed increased levels of L-arginine, supporting the uptake of amino acids by increased levels of Cat2 and Cat1 mRNA expression [23]. Excess L-arginine availability to the macrophages, which was not metabolized via arginase (absent in parasite) or agmatinase that was not modulated in amastigotes axenic forms, could cause the presence of higher levels of L-argininic acid [37]. Additionally, the absence of parasite arginase reduced the levels of ornithine (as compared to La-WT infected macrophages), although the increased levels of Arg1 in infected La-arg − -macrophages when compared to infected with La-WT [23], highlighting the impact of parasite arginase into the total of L-arginine conversion to ornithine.…”
Section: Discussionmentioning
confidence: 88%
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“…In contrast, BALB/c infection with the La-arg − parasite showed increased levels of L-arginine, supporting the uptake of amino acids by increased levels of Cat2 and Cat1 mRNA expression [23]. Excess L-arginine availability to the macrophages, which was not metabolized via arginase (absent in parasite) or agmatinase that was not modulated in amastigotes axenic forms, could cause the presence of higher levels of L-argininic acid [37]. Additionally, the absence of parasite arginase reduced the levels of ornithine (as compared to La-WT infected macrophages), although the increased levels of Arg1 in infected La-arg − -macrophages when compared to infected with La-WT [23], highlighting the impact of parasite arginase into the total of L-arginine conversion to ornithine.…”
Section: Discussionmentioning
confidence: 88%
“…The genomic and transcriptomic networks during Leishmania infection have been providing interesting data regarding how Leishmania can to modulate the gene organization and gene expression of its host [37][38][39][40]. Additionally, we have observed differentially expressed genes profile during the different phases of parasite growth [37,38,41]. The metabolomic profile is poorly explored and it could help to understand the metabolite's consummation and network systems pathway of both the host and parasite.…”
Section: Discussionmentioning
confidence: 98%
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“…The L. major entry for DRBD3 (LmjF.04.1170) on TriTrypDB indicates that the gene is constitutively expressed between parasite life-cycle stages, is not under immune 100 pressure, and has minimal sequence variation between species (17). RNA-seq experiments in both L. major and L. amazonensis have demonstrated little to no change in expression of this gene between the promastigote and amastigote stage consistent with the characteristics of a constitutively expressed housekeeping gene (18)(19)(20). Additionally, there were no identified epitopes in the Immune Epitope Database (IEDB) corresponding to DRBD3 peptides, suggesting 105 that it is not likely to be influenced by host immune pressure (21).…”
Section: Rt-pcr Assay Design For the Leishmania Rna Binding Protein mentioning
confidence: 99%