RNA-seq Reveals the Overexpression of IGSF9 in Endometrial Cancer

Abstract

We performed RNA-seq on an Illumina platform for 7 patients with endometrioid endometrial carcinoma for which both tumor tissue and adjacent noncancer tissue were available. A total of 66 genes were differentially expressed with significance level at adjusted p value < 0.01. Using the gene functional classification tool in the NIH DAVID bioinformatics resource, 5 genes were found to be the only enriched group out of that list of genes. The gene IGSF9 was chosen for further characterization with immunohistochemical staining of a larger cohort of human endometrioid carcinoma tissues. The expression level of IGSF9 in cancer cells was significantly higher than that in control glandular cells in paired tissue samples from the same patients (p = 0.008) or in overall comparison between cancer and the control (p = 0.003). IGSF9 expression is higher in patients with myometrium invasion relative to those without invasion (p = 0.015). Reanalysis of RNA-seq dataset from The Cancer Genome Atlas shows higher expression of IGSF9 in endometrial cancer versus normal control and expression was associated with poor prognosis. These results suggest IGSF9 as a new biomarker in endometrial cancer and warrant further studies on its function, mechanism of action, and potential clinical utility.

Multi-gene models for survival prognosis of colorectal cancer patients

“…IGSF9 was found to be associated with the prognosis of nasopharyngeal carcinoma and that downregulation of IGSF9 expression reduced the proliferation, migration and invasion of nasopharyngeal carcinoma cells, and that hypoxia induced IGSF9 expression [44,45] . The microbiome, metabolome and host DNA methylation of the intestinal mucosa of CRC patients are associated with changes in IGSF9 expression, and they in uence the development of CRC through their interactions [44,46,47] . Comprehensive bioinformatics analysis has identi ed PCOLCE2 as a relevant biomarker for the diagnosis and prognosis of CRC patients, providing some guidance for further exploration of potential biomarkers for diagnosis and prognosis prediction in CRC patients [48] .…”

Integrated analysis of gene expression and DNA methylation datasets identified key genes and a 6-gene prognostic signature for primary lung adenocarcinoma

“…Additionally, carcinoma-associated fibroblasts play a crucial role in maintaining an optimal cancer microenvironment for cell proliferation and survival ( Cirri and Chiarugi, 2012 ; Marsh et al, 2013 ). VSIG2 has been found to be differentially expressed in endometrial cancer ( Shi et al, 2018 ), and to be significantly associated with bladder cancer risk ( Moore et al, 2010 ). Since the 6-gene signature is established based on the hypomethylation-related risky genes and hypermethylation-related protective genes, it can provide new ideas for methylation-based treatment for LUAD.…”

Identified IGSF9 association with prognosis and hypoxia in nasopharyngeal carcinoma by bioinformatics analysis

“…Meanwhile, we verified that IGSF9 promoted the proliferation, migration and invasion of NPC cells in vitro. To date, there is one published report [ 27 ] on over-expression of IGSF9 associated with poor PFS in endometrial cancer tissue. Similar result was found in NPC tissue in our research.…”