RNA-seq reveals disruption in honey bee gene regulation when caged and deprived of hive conditions

Alburaki, Mohamed; Karim, Shahid; Lamour, Kurt; Adamczyk, John J.; Stewart, Scott

doi:10.1242/jeb.207761

Cited by 11 publications

(14 citation statements)

References 58 publications

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“…However, Sivanto™ exposed honey bees consumed significantly more sugar syrup than the control honey bees over the 10-day period. Pesticide exposures are known to alter sugar syrup consumption in treated bees [19,41]. The energetics of carbohydrate metabolism and regulation may be responsible for such consumption differences.…”

Section: Discussionmentioning

confidence: 99%

“…We examined survival at 6 hours (lethal effects) and 10 days (sub-lethal effects) in two separate contact exposure experiments. Since pesticide exposures also have the potential to alter food consumption in treated bees [19,41] and affect carbohydrate metabolism, we also measured sugar syrup consumptions. Additionally, physiological impacts were assessed by estimating both oxidative stress (ROS/RNS assay) and apoptosis (caspase-3 protein activity) in the treatment groups.…”

Section: Introductionmentioning

confidence: 99%

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Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

et al. 2020

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Pesticide exposures can have detrimental impacts on bee pollinators, ranging from immediate mortality to sub-lethal impacts. Flupyradifurone is the active ingredient in Sivanto™ and sulfoxaflor is the active ingredient in Transform ®. They are both relatively new insecticides developed with an intent to reduce negative effects on bees, when applied to bee-attractive crops. With the growing concern regarding pollinator health and pollinator declines, it is important to have a better understanding of any potential negative impacts, especially sublethal, of these pesticides on bees. This study reports novel findings regarding physiological stress experienced by bees exposed to field application rates of these two insecticides via a Potter Tower sprayer. Two contact exposure experiments were conducted-a shorter 6hour study and a longer 10-day study. Honey bee mortality, sugar syrup and water consumption, and physiological responses (oxidative stress and apoptotic protein assays) were assessed in bees exposed to Sivanto™ and Transform ® , and compared to bees in control group. For the longer, 10-day contact exposure experiment, only the Sivanto™ group was compared to the control group, as high mortality recorded in the sulfoxaflor treatment group during the shorter contact exposure experiment, made the latter group unfeasible to test in the longer 10-days experiment. In both the studies, sugar syrup and water consumptions were significantly different between treatment groups and controls. The highest mortality was observed in Transform ® exposed bees, followed by the Sivanto™ exposed bees. Estimates of reactive oxygen/nitrogen species indicated significantly elevated oxidative stress in both pesticide treatment groups, when compared to controls. Caspase-3 protein assays, an indicator of onset of apoptosis, was also significantly higher in the pesticide treatment groups. These differences were largely driven by post exposure duration, indicating sublethal impacts. Further, our findings also emphasize the need to revisit contact exposure impacts of Sivanto™, given the sub-lethal impacts and mortality observed in our long-term (10-day) contact exposure experiment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

et al. 2020

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“…Kobayashi of different SOD genes within silk moth (B. mori) under various oxidative stressors and found Rsod gene (BmSOD6) is mostly expressed within the testes on day 3 of fifth instar larvae, indicating a role in removing ROS generated during spermatogenesis [47]. Additionally, honeybees (Apis mellifera) exposed to caging stress for up to 4 weeks showed a constant upregulation of only two genes, namely Rsod and another antioxidant thioredoxin-1 (Trx-1) [48]. In crown of thorns starfish, A. planci, maintained in captivity, one Rsod homologue (gbr.190.13.t1) is upregulated in three tissues (skin, tube feet and spines), and a second homologue (gbr.190.14.t1) in skin only, compared to wild populations [49].…”

Section: An Atypical Cuznsod -Rsod -Is Widespread In the Animal Kingdommentioning

confidence: 99%

Distribution and diversity of ROS-generating enzymes across the animal kingdom, with a focus on sponges (Porifera)

Hewitt

Degnan

2022

BMC Biol

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Background Reactive derivatives of oxygen (reactive oxygen species; ROS) are essential in signalling networks of all aerobic life. Redox signalling, based on cascades of oxidation–reduction reactions, is an evolutionarily ancient mechanism that uses ROS to regulate an array of vital cellular processes. Hydrogen peroxide (H2O2) and superoxide anion (O2•−) are employed as signalling molecules that alter the oxidation state of atoms, inhibiting or activating gene activity. Here, we conduct metazoan-wide comparative genomic assessments of the two enzyme families, superoxide dismutase (SOD) and NADPH oxidases (NOX), that generate H2O2 and/or O2•− in animals. Results Using the genomes of 19 metazoan species representing 10 phyla, we expand significantly on previous surveys of these two ancient enzyme families. We find that the diversity and distribution of both the SOD and NOX enzyme families comprise some conserved members but also vary considerably across phyletic animal lineages. For example, there is substantial NOX gene loss in the ctenophore Mnemiopsis leidyi and divergent SOD isoforms in the bilaterians D. melanogaster and C. elegans. We focus particularly on the sponges (phylum Porifera), a sister group to all other metazoans, from which these enzymes have not previously been described. Within Porifera, we find a unique calcium-regulated NOX, the widespread radiation of an atypical member of CuZnSOD named Rsod, and a novel endoplasmic reticulum MnSOD that is prevalent across aquatic metazoans. Conclusions Considering the precise, spatiotemporal specificity of redox signalling, our findings highlight the value of expanding redox research across a greater diversity of organisms to better understand the functional roles of these ancient enzymes within a universally important signalling mechanism.

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“…The honeybees were obtained as previously described [29,30]. Two frames with sealed broods nearing adult emergence were collected from an apparently healthy colony at the Institute of Apiculture Research, Anhui Agriculture University, Hefei, China.…”

Section: Honeybee Rearingmentioning

confidence: 99%

Transcriptomic analysis to elucidate the response of honeybees (Hymenoptera: Apidae) to amitraz treatment

et al. 2020

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Amitraz is an acaricide that is widely used in apiculture. Several studies have reported that in honeybees (Apis mellifera Linnaeus; Hymenoptera: Apidae), amitraz affects learning, memory, behavior, immunity, and various other physiological processes. Despite this, few studies have explored the molecular mechanisms underlying the action of amitraz on honeybees. Here, we investigated the transcriptome of honeybees after exposure to 9.4 mg/L amitraz for 10 d, a subchronic dose. Overall, 279 differentially expressed genes (DEGs) were identified (237 upregulated, 42 downregulated). Several, including Pla2,

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RNA-seq reveals disruption in honey bee gene regulation when caged and deprived of hive conditions

Cited by 11 publications

References 58 publications

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

Distribution and diversity of ROS-generating enzymes across the animal kingdom, with a focus on sponges (Porifera)

Transcriptomic analysis to elucidate the response of honeybees (Hymenoptera: Apidae) to amitraz treatment

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