RNA-seq profiles of chicken type II pneumocyte in response to Escherichia coli infection

Peng, Lu-Yuan; Cui, Zhen-Qiang; Wu, Zong-Mei; Fu, Binying; Yi, Pengfei; Shen, Haiqing

doi:10.1371/journal.pone.0217438

Cited by 7 publications

(5 citation statements)

References 45 publications

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“…To explore the host–pathogen interaction, a response in global gene expression profiling of chicken type II pneumocytes (CP II cells), responsible for secreting surfactants and modulating lung immunity, to avian pathogenic Escherichia coli (APEC-O78) infection was determined. In fact, CP II cells were shown to respond to APEC infection with marked changes in the expression of 1390 genes (from 18,996 genes identified) with 803 downregulated mRNAs and 587 upregulated mRNAs [ 266 ]. The major enriched pathways were identified to be related to the NF-κB signaling pathway, apoptosis pathway, tight junction, and cytokine–cytokine receptor interaction.…”

Section: Effect Of Various Stress Factors On Nf-κb Expression Andmentioning

confidence: 99%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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Redox biology is a very quickly developing area of modern biological sciences, and roles of redox homeostasis in health and disease have recently received tremendous attention. There are a range of redox pairs in the cells/tissues responsible for redox homeostasis maintenance/regulation. In general, all redox elements are interconnected and regulated by various means, including antioxidant and vitagene networks. The redox status is responsible for maintenance of cell signaling and cell stress adaptation. Physiological roles of redox homeostasis maintenance in avian species, including poultry, have received limited attention and are poorly characterized. However, for the last 5 years, this topic attracted much attention, and a range of publications covered some related aspects. In fact, transcription factor Nrf2 was shown to be a master regulator of antioxidant defenses via activation of various vitagenes and other protective molecules to maintain redox homeostasis in cells/tissues. It was shown that Nrf2 is closely related to another transcription factor, namely, NF-κB, responsible for control of inflammation; however, its roles in poultry have not yet been characterized. Therefore, the aim of this review is to describe a current view on NF-κB functioning in poultry with a specific emphasis to its nutritional modulation under various stress conditions. In particular, on the one hand, it has been shown that, in many stress conditions in poultry, NF-κB activation can lead to increased synthesis of proinflammatory cytokines leading to systemic inflammation. On the other hand, there are a range of nutrients/supplements that can downregulate NF-κB and decrease the negative consequences of stress-related disturbances in redox homeostasis. In general, vitagene–NF-κB interactions in relation to redox balance homeostasis, immunity, and gut health in poultry production await further research.

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Section: Effect Of Various Stress Factors On Nf-κb Expression Andmentioning

confidence: 99%

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

2021

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“…It was shown in vitro that embryonic primary type II pneumocytes belonging to the squamous epithelial cells possess important roles as an entry barrier for pathogens including APEC and as modulators of the local immune response. The transcriptome of primary chicken type II pneumocytes after stimulation with a high dose of APEC O78 (a multiplicity of infection (MOI) of 100) suggested that the major enriched pathways were related to the NF-κB signalling pathway, apoptosis, tight junctions, and cytokine-cytokine receptor interactions, with IL1B, IL6 and IL18 upregulated (Peng et al, 2019b). Several plant-derived substances can modulate the type II pneumocyte immune response to APEC, for example rutin, astragaloside IV, α-cyperone, baicalin, and schizandrin may reduce cell damage of type II cells after APEC O78 exposure (Zhang et al, 2014;Zhang et al, 2016;Peng et al, 2018b;Peng et al, 2019a;Peng et al, 2019c;Yuan et al, 2020a;Yuan et al, 2020b).…”

Section: The Epithelial Barriermentioning

confidence: 99%

The bird’s immune response to avian pathogenic Escherichia coli

2021

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Avian pathogenic Escherichia coli (APEC) cause colibacillosis in birds, a syndrome of severe respiratory and systemic disease that constitutes a major threat due to early mortality, condemnation of carcasses and reduced productivity. APEC can infect different types of birds in all commercial settings, and birds of all ages although disease tends to be more severe in younger birds likely a consequence of an immature immune system. APEC can act as both primary and secondary pathogens, with predisposing factors for secondary infections including poor housing conditions, respiratory viral and Mycoplasma spp. infections or vaccinations. Controlled studies with APEC as a primary pathogen have been used to study the bird's immune response to APEC, although it may not always be representative of natural infections which may be more complex due to the presence of secondary agents, stress and environmental factors. Under controlled experimental conditions, a strong early innate immune response is induced which includes host defence peptides in mucus and

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“…High-throughput sequencing techniques have become powerful and effective tools for gaining a deeper understanding of the basic molecular mechanisms of complicated systems ( Wang et al, 2009 ; Ozsolak and Milos, 2011 ). RNA sequencing, a high-throughput technique, has been generally used in livestock to find the expression patterns of functional genes ( Cui et al, 2017 ; Ni et al, 2019 ; Peng et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Identifies Candidate Genes and Signaling Pathways Associated With Feed Efficiency in Xiayan Chicken

et al. 2021

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Feed efficiency is an important economic factor in poultry production, and the rate of feed efficiency is generally evaluated using residual feed intake (RFI). The molecular regulatory mechanisms of RFI remain unknown. Therefore, the objective of this study was to identify candidate genes and signaling pathways related to RFI using RNA-sequencing for low RFI (LRFI) and high RFI (HRFI) in the Xiayan chicken, a native chicken of the Guangxi province. Chickens were divided into four groups based on FE and sex: LRFI and HRFI for males and females, respectively. We identified a total of 1,015 and 742 differentially expressed genes associated with RFI in males and females, respectively. The 32 and 7 Gene Ontology (GO) enrichment terms, respectively, identified in males and females chiefly involved carbohydrate, amino acid, and energy metabolism. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 11 and 5 significantly enriched signaling pathways, including those for nutrient metabolism, insulin signaling, and MAPK signaling, respectively. Protein–protein interaction (PPI) network analysis showed that the pathways involving CAT, ACSL1, ECI2, ABCD2, ACOX1, PCK1, HSPA2, and HSP90AA1 may have an effect on feed efficiency, and these genes are mainly involved in the biological processes of fat metabolism and heat stress. Gene set enrichment analysis indicated that the increased expression of genes in LRFI chickens was related to intestinal microvilli structure and function, and to the fat metabolism process in males. In females, the highly expressed set of genes in the LRFI group was primarily associated with nervous system and cell development. Our findings provide further insight into RFI regulation mechanisms in chickens.

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RNA-seq profiles of chicken type II pneumocyte in response to Escherichia coli infection

Cited by 7 publications

References 45 publications

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

Redox Homeostasis in Poultry: Regulatory Roles of NF-κB

The bird’s immune response to avian pathogenic Escherichia coli

Transcriptome Analysis Identifies Candidate Genes and Signaling Pathways Associated With Feed Efficiency in Xiayan Chicken

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