RNA-seq data from mature male gonads of marine mussels Mytilus edulis and M. galloprovincialis

Diz, Angel P.; Romero, M.; Pérez‐Figueroa, Andrés; Swanson, Willie J.; Skibinski, D. O. F.

doi:10.1016/j.dib.2018.09.086

Cited by 9 publications

(16 citation statements)

References 12 publications

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“…Extended versions of Material and Methods for RNA-seq and proteomic analysis are provided in Ref. [47] and File S1 respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The quality control and filtering of nucleotide sequences was carried out as explained in Ref. [47], yielding 187,829,361 confident reads that were used for de novo assembly and generation of a consensus transcriptome.…”

Section: Rna Extraction Mrna Library and Illumina Paired-end Sequencmentioning

confidence: 99%

“…using Fisher's exact test with a FDR=5% (see Ref. [47] for further details on method). This might provide some clues about the differences at functional level present in mature male gonad tissue of the two Mytilus spp.…”

Section: De Novo Transcriptome Assembly and Functional Annotation-due Tomentioning

confidence: 99%

“…Functional annotation and an enrichment analysis for those differentially expressed transcripts was carried out as explained in the above section 2.1.3 and Ref. [47].…”

Section: Differential Expression Analyses-mentioning

confidence: 99%

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RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

Romero

Pérez‐Figueroa

Carrera

et al. 2019

Journal of Proteomics

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Model systems for the study of fertilization include marine invertebrates with external fertilisation, such as abalones, sea urchins and mussels, because of the ease with which large quantities of gametes released into seawater can be collected after induced spawning. Unlike abalones and sea urchins, hybridisation has been reported between mussels of different Mytilus spp., which thus makes them very appealing for the study of reproductive isolation at both pre- and postzygotic levels. There is a lack of empirical proteomic studies on sperm samples comparing different Mytilus species, which could help to advance this study. A comparative analysis of sperm proteomes across different taxa may provide important insights into the fundamental molecular processes and mechanisms involved in reproductive isolation. It might also contribute to a better understanding of sperm function and of the adaptive evolution of sperm proteins in different taxa. There is now growing evidence from genomics studies that multiple protein complexes and many individual proteins might have important functions in sperm biology and the fertilisation process. From an applied perspective, the identification of sperm-specific proteins could also contribute to the improved understanding of fertility problems and as targets for fertility control.

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“…Extended versions of Material and Methods for RNA-seq and proteomic analysis are provided in Ref. [47] and File S1 respectively.…”

Section: Methodsmentioning

confidence: 99%

Section: Rna Extraction Mrna Library and Illumina Paired-end Sequencmentioning

confidence: 99%

Section: De Novo Transcriptome Assembly and Functional Annotation-due Tomentioning

confidence: 99%

“…Functional annotation and an enrichment analysis for those differentially expressed transcripts was carried out as explained in the above section 2.1.3 and Ref. [47].…”

Section: Differential Expression Analyses-mentioning

confidence: 99%

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RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

Romero

Pérez‐Figueroa

Carrera

et al. 2019

Journal of Proteomics

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“…For a few samples, the RNA integrity was analyzed using the Bioanalyzer 2100 (Agilent Technologies, USA) also. Due to the presence of the 18 s peak only, as for other protostomes (Diz et al 2018), the instrument was not able to return the R.I.N. value; however, the presence of a net peak still indicates an intact RNA.…”

Section: Rna Integrity and Retro-transcriptionmentioning

confidence: 98%

Molecular and biochemical responses of vitellogenin in the mussel Mytilus galloprovincialis exposed to the glyphosate-based herbicide Roundup® Power 2.0

Fabrello

Grapputo

Munari

et al. 2020

Environ Sci Pollut Res

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Glyphosate-based herbicides (GBHs) occur in aquatic ecosystems at concentrations of hundreds of micrograms per liter. As formulation adjuvants are suspected to be endocrine-disrupting chemicals, we assessed the effects of the recent GBH formulation Roundup® Power 2.0 on vitellogenin (VTG) in Mytilus galloprovincialis. Mussels were exposed for 7, 14, and 21 days to two concentrations of the commercial formulation, corresponding to 100 and 1000 μg/L of glyphosate. The expression of the vtg gene in gonads of females and males, as well as the levels of alkali labile phosphates (ALP) in gonads and non-gonadal tissues from the two sexes were measured. No significant alterations were observed in vtg expression values during the exposure. Conversely, a significant reduction in gonadal ALP levels was observed in females exposed for 21 days and in males exposed for 7 days. In addition, ALP levels increased significantly in gonads from males exposed for 21 days to the two concentrations of Roundup®. As for non-gonadal tissues, ALP levels did not change significantly in females, whereas ALP levels decreased significantly in non-gonadal tissues from males exposed for 21 days to the lowest concentration tested. An overall statistically significant difference in ALP levels was found between females and males. Although preliminary, our study suggests that GBH can affect reproduction-related parameters in mussels.

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A Primer and Guidelines for Shotgun Proteomic Analysis in Non-model Organisms

Diz

Sánchez-Marín

2021

Shotgun Proteomics

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RNA-seq data from mature male gonads of marine mussels Mytilus edulis and M. galloprovincialis

Cited by 9 publications

References 12 publications

RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

Molecular and biochemical responses of vitellogenin in the mussel Mytilus galloprovincialis exposed to the glyphosate-based herbicide Roundup® Power 2.0

A Primer and Guidelines for Shotgun Proteomic Analysis in Non-model Organisms

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