RNA-Seq-based transcriptome analysis of Korean rose bitterling (Rhodeus uyekii) exposed to synthetic estrogen 17-α-ethinylestradiol (EE2)

Kong, Hee Jeong; Lee, Il Kyu; Kim, Julan; Kim, Woo Jin; Kim, Hyung Soo; Cho, Wang Sik; Kim, Dong-Wook; Park, Jung Youn; An, Cheul Min

doi:10.1016/j.margen.2015.06.005

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…RNA-Seq of the Korean rose bitterling ( Rhodeus uyekii ) indicated that exposure to 100 ng/L of EE2 for seven days induced expression of apoptotic genes, including p53 and TNF-α, and cancer genes, including Smad2 and CAV2 in hepatic and skin tissues [ 103 ].…”

Section: Environmental and Human Health Implications Of Edc Exposurementioning

confidence: 99%

Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes

Verga

Huff

Owens

et al. 2022

IJERPH

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Exposure to Endocrine Disrupting Chemicals (EDC) has been linked with several adverse outcomes. In this review, we examine EDCs that are pervasive in the environment and are of concern in the context of human, animal, and environmental health. We explore the consequences of EDC exposure on aquatic life, terrestrial animals, and humans. We focus on the exploitation of genomics technologies and in particular whole transcriptome sequencing. Genome-wide analyses using RNAseq provides snap shots of cellular, tissue and whole organism transcriptomes under normal physiological and EDC perturbed conditions. A global view of gene expression provides highly valuable information as it uncovers gene families or more specifically, pathways that are affected by EDC exposures, but also reveals those that are unaffected. Hypotheses about genes with unknown functions can also be formed by comparison of their expression levels with genes of known function. Risk assessment strategies leveraging genomic technologies and the development of toxicology databases are explored. Finally, we review how the Adverse Outcome Pathway (AOP) has exploited this high throughput data to provide a framework for toxicology studies.

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Section: Environmental and Human Health Implications Of Edc Exposurementioning

confidence: 99%

Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes

Verga

Huff

Owens

et al. 2022

IJERPH

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“…R. uyekii β-actin gene was suggested as a promoter capable of driving constitutive transgene expression [ 21 ]. It is worth studying the stress response of R. uyekii since fish may need to control potential damage(s) in the water [ 22 , 23 ]. Antioxidant protein peroxiredoxin 1 have been cloned and characterized in R. uyekii [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular and Functional Characterization of Thioredoxin 1from Korean Rose Bitterling (Rhodeus uyekii)

Kim

Moon

Kim

et al. 2015

IJMS

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Thioredoxin is a multifunctional antioxidant enzyme that belongs to the reductase family. In this study, we cloned and characterized thioredoxin 1 cDNA from the Korean rose bitterling Rhodeus uyekii (RuTrx). The full-length RuTrx cDNA consists of 674 bp with a 324 nt open reading frame (ORF) encoding a 107 aa protein. The deduced RuTrx amino acid sequence indicated a characteristic redox active site, 31WCGPC35. Pairwise alignment revealed RuTrx amino acid identity (55.1%–83.2%) with orthologs from various species of mammalia, amphibia, fish and bird. Phylogenetic analysis was conducted to determine the evolutionary position of RuTrx. Expression analysis showed that RuTrx transcripts were present in all of the tissues examined, and was high in the hepatopancreas of R. uyekii. During early development, the expression of RuTrx transcripts was increased. Recombinant RuTrx protein (rRuTrx) was tested for its capacity to serve as an antioxidant enzyme using a metal-catalyzed oxidation (MCO) system. The ability of rRuTrx to protect against supercoiled DNA cleavage due to oxidative nicking increased in a dose-dependent manner. In Raw264.7 cells, Dihydroethidium (DHE) staining for ROS production indicated the antioxidant activity of rRuTrx. Together, these findings suggest that RuTrx may play a role in maintaining the redox state balance in Korean rose bitterling R. uyekii.

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Transcriptome Analysis of Aquatic Species Exposed to Endocrine Disruptors

Agarwal

Rathipriya

Rather

et al. 2023

Xenobiotics in Aquatic Animals

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RNA-Seq-based transcriptome analysis of Korean rose bitterling (Rhodeus uyekii) exposed to synthetic estrogen 17-α-ethinylestradiol (EE2)

Cited by 8 publications

References 20 publications

Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes

Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes

Molecular and Functional Characterization of Thioredoxin 1from Korean Rose Bitterling (Rhodeus uyekii)

Transcriptome Analysis of Aquatic Species Exposed to Endocrine Disruptors

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