RNA-Seq Analysis Reveals the Role of Omp16 in Brucella-Infected RAW264.7 Cells

Abstract

Brucellosis is an endemic zoonotic infectious disease in the majority of developing countries, which causes huge economic losses. As immunogenic and protective antigens at the surface of Brucella spp., outer membrane proteins (Omps) are particularly attractive for developing vaccine and could have more relevant role in host–pathogen interactions. Omp16, a homolog to peptidoglycan-associated lipoproteins (Pals), is essential for Brucella survival in vitro. At present, the functions of Omp16 have been poorly studied. Here, the gene expression profile of RAW264.7 cells infected with Brucella suis vaccine strain 2 (B. suis S2) and ΔOmp16 was analyzed by RNA-seq to investigate the cellular response immediately after Brucella entry. The RNA-sequence analysis revealed that a total of 303 genes were significantly regulated by B. suis S2 24 h post-infection. Of these, 273 differentially expressed genes (DEGs) were upregulated, and 30 DEGs were downregulated. These DEGs were mainly involved in innate immune signaling pathways, including pattern recognition receptors (PRRs), proinflammatory cytokines, and chemokines by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. In ΔOmp16-infected cells, the expression of 52 total cells genes was significantly upregulated and that of 9 total cells genes were downregulated compared to B. suis S2-infected RAW264.7 cells. The KEGG pathway analysis showed that several upregulated genes were proinflammatory cytokines and chemokines, such as interleukin (IL)-6, IL-11, IL-12β, C–C motif chemokine (CCL2), and CCL22. All together, we clearly demonstrate that ΔOmp16 can alter macrophage immune-related pathways to increase proinflammatory cytokines and chemokines, which provide insights into illuminating the Brucella pathogenic strategies.

Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17

“…The functional enrichment analyses of the DE mRNAs and target genes of the DE lncRNAs showed that immune-related terms were enriched for the upregulated DE mRNAs and lncRNAs, and that metabolic-related terms were enriched for the downregulated DE mRNAs and DE lncRNAs. Similar results have been reported previously ( 66 , 67 ), further verifying our hypothesis that that the activity of immune-related genes was increased in the AN lambs and that the metabolic homeostasis was severely disrupted in the SE lambs during E. coli F17 infection. Notably, the PPAR signaling pathway was found enriched in KEGG enrichment analysis based on both DE lncRNAs and DE mRNAs.…”

Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to Brucella Melitensis strain M5-90

“…MiR-744 is known to be associated with the innate immunity pathway and acts as a tumor suppressor, thereby inhibiting cell proliferation and promoting cell apoptosis ( Pilson et al, 2020 ; Yuan et al, 2020 ). Meanwhile, the cytokine encoded genes, including IL-6 , IL-7 , IL-11 , IL-23A and IL-24 , were significantly changed in our RNA-seq data, which were partially consistent with previous studies ( Avila-Calderón et al, 2020 ; Zhou et al, 2021 ). IL-6 is a pleiotropic, pro-inflammatory cytokine produced by a variety of cell types, including lymphocytes, monocytes, and fibroblasts.…”

A gene expression map of host immune response in human brucellosis

“…The interaction between Brucella and host immune system is critical for the development of persistent infection or infection clearance (8,18). To date, transcriptomic data derived from Brucella-infected mouse macrophages or mouse cell lines, domestic ruminants or Brucella-vaccinated animals (19)(20)(21)(22)(23)(24)(25). This study analyses, for the first time, the transcriptome profile, both in vitro, in Brucellainfected primary Mφ and PMNs, and ex-vivo, in PBMCs and PMNs derived from patients with acute brucellosis before and after treatment.…”