RNA-Seq Analysis Reveals Candidate Targets for Curcumin againstTetranychus cinnabarinus

Abstract

Tetranychus cinnabarinus is an important agricultural pest with a broad host range. We previously identified curcumin as a promising acaricidal compound against T. cinnabarinus. However, the acaricidal mechanism of curcumin remains unknown. In this study, RNA-seq was employed to analyze the transcriptome changes in T. cinnabarinus treated with curcumin or the solvent. A total of 105,706,297 clean sequence reads were generated by sequencing, with more than 90% of the reads successfully mapped to the reference sequence. The RNA-seq identified 111 and 96 differentially expressed genes between curcumin- and solvent-treated mites at 24 and 48 h after treatment, respectively. GO enrichment analysis of differentially expressed genes showed that the cellular process was the dominant group at both time points. Finally, we screened 23 differentially expressed genes that were functionally identical or similar to the targets of common insecticide/acaricides or genes that were associated with mite detoxification and metabolism. Calmodulin, phospholipase A2, and phospholipase C were activated upon curcumin treatment suggesting that the calcium channel related genes might play important roles in mite's response to curcumin. Overall our results revealed the global transcriptional changes in T. cinnabarinus after curcumin treatment to enable further identification of the targets of curcumin in mites.

Curcumin affects gene expression and reactive oxygen species via a PKA dependent mechanism in Dictyostelium discoideum

“…Genome wide analysis including microarrays, RNAseq, and proteomics have been used previously to examine the effect of curcumin alone, or in combination with other compounds, in a variety of cell and tissue types (see review [ 82 ]). Examples of RNAseq studies include the effect of curcumin on a mouse model of colorectal cancer [ 83 ], parasitic mites [ 84 ], and breast cancer stem cells [ 85 ]. Microarray studies characterized the effect of curcumin on lung [ 86 , 87 ] and breast [ 88 ] cancer cell lines.…”

Using Next-Generation Sequencing to Detect Differential Expression Genes in Bradysia odoriphaga after Exposure to Insecticides

“…Next generation sequencing (NGS) technologies have dramatically developed in the last decade, which allows us to sequence DNA and RNA much more quickly and cheaply than before, and as such has revolutionized the study of genomics and molecular biology [ 9 , 10 , 11 ]. RNA-Seq has been used to find insecticide-metabolism-related genes in several species, such as Cimex lectularius [ 12 ], Plutella xylostella [ 13 ], Panonychus ulmi [ 14 ] and Tetranychus cinnabarinus [ 15 ].…”

Effect of acaricidal compound extracted from Arachis hypogaea Linn against Tetranychus cinnabarinus

“…Recently, extracts of various plants have been shown to possess acaricidal activities against T. cinnabarinus , such as those of Mentha piperita (Ren et al, ), Curcuma longa L. (Liu et al, ), Inula britanica (Ma et al, ), Chenopodium quinoa Willd (Harder, Tello, & Giliomee, ), Solanum nigrum Linn (Chen & Dai, ), Aloe vera L. (Zhang, Zhang, Luo, & Ding, ), Lactuca sativa (Li et al, ) and Polygonum aviculare (Cheng, Wang, Ma, Liu, & Shi, ) . These acaricidal plants influence the growth and development of T. cinnabarinus through repellence, antifeeding effects or oviposition deterrence (Chen & Dai, ).…”