RNA-Seq Analysis Reveals a Positive Role of HTR2A in Adipogenesis in Yan Yellow Cattle

Abstract

In this study, we performed high throughput RNA sequencing at the primary bovine preadipocyte (Day-0), mid-differentiation (Day-4), and differentiated adipocyte (Day-9) stages in order to characterize the transcriptional events regulating differentiation and function. The preadipocytes were isolated from subcutaneous fetal bovine adipose tissues and were differentiated into mature adipocytes. The adipogenic characteristics of the adipocytes were detected during various stages of adipogenesis (Day-0, Day-4, and Day-9). We used RNA sequencing (RNA-seq) to investigate a comprehensive transcriptome information of adipocytic differentiation. Compared to the pre-differentiation stage (Day-0), 2510 genes were identified as differentially expressed genes (DEGs) at the mid-differentiation stage (Day-4). We found 2446 DEGs in the mature adipocytic stage relative to the mid-differentiation stage. Some adipogenesis-related transcription factors, CCAAT-enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ) were differentially expressed at Day-0, Day-4, and Day-9. We further investigated the adipogenic function of 5-hydroxytryptamine receptor 2A (HTR2A) in adipogenesis. Overexpression of HTR2A stimulated the differentiation of preadipocytes, and knockdown of HTR2A had opposite effects. Furthermore, functional enrichment analysis of DEGs revealed that the PI3K-Akt signaling pathway was the significantly enriched pathway, and HTR2A regulated adipogenesis by activating or inhibiting phosphorylation of phospho-AKT (Ser473). In summary, the present study provides the first comparative transcription of various periods of adipocytes in cattle, which presents a solid foundation for further study into the molecular mechanism of fat deposition and the improvement of beef quality in cattle.

Genetic Parameters and Genome-Wide Association Studies of Quality Traits Characterised Using Imaging Technologies in Rainbow Trout, Oncorhynchus mykiss

“…A GWAS in sheep linked a SNP close to htr1e with internal fat content ( Hernández-Montiel et al, 2020 ). In Yan Yellow cattle, RNA-seq analysis revealed a positive role for another serotonin receptor, htr2a , in adipogenesis ( Yun et al, 2018 ). In humans, genetic studies have reported that polymorphisms in Htr genes (for example, HTR1A , HTR1Dβ , and HTR2A ) are associated with obesity ( Gorwood et al, 2002 ; Levitan et al, 2006 ; Compan et al, 2012 ; Oh et al, 2015 ).…”

Molecular Mechanism of Stem Cell Differentiation into Adipocytes and Adipocyte Differentiation of Malignant Tumor

“…RNA sequence analysis showed that KLFl6 expression was decreased on the first day of adipocyte differentiation of 3T3-L1 cells. Adipocyte differentiation was promoted by KLF16 knockdown but was inhibited by KLF16 overexpression via inhibition of PPAR γ promoter activity [ 64 ]. In addition, KLF3 and KLF7 were also found to play a negative regulatory role in adipocyte differentiation [ 65 , 66 ].…”

RNA-Seq Analysis Identifies Differentially Expressed Genes in Subcutaneous Adipose Tissue in Qaidaford Cattle, Cattle-Yak, and Angus Cattle

“…The PI3K-Akt signaling pathway is a classic insulin signaling pathway [26], and is involved in proliferation, differentiation, apoptosis, and glucose transport. Moreover, the PI3K-Akt pathway may also regulate adipogenesis [27,28]. The detailed function and regulatory mechanisms of the differentially expressed genes involved in these pathways should be carefully elucidated in subsequent studies.…”