RNA·RNA and Protein·RNA Interactions During the Initiation of Protein Synthesis

Steitz, Joan A.; Sprague, Karen U.; Steege, Deborah A.; Yuan, Robert; Laughrea, Michael; Moore, Peter B.; Wahba, Amy

doi:10.1016/b978-0-12-722560-9.50032-9

Cited by 11 publications

(11 citation statements)

References 66 publications

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“…RNA duplex with the polypyrimidine portion of the 3' terminus of 16-S rRNA. Demonstration [2] of an RNA . RNA duplex between the RNA sequence corresponding to the R17 A protein and the colicine fragment of 16-S rRNA has been accomplished.…”

Section: Discussionmentioning

confidence: 99%

“…Intercistronic regions are thought to be involved in the ribosomal recognition of starter triplets [ I , 2,5]. Over 20 regions, recognized by E. coli ribosomes, have now been sequenced [2]. Close inspection shows the occurrence of nonsense codons at short variable distances from the initiator [7].…”

Section: Discussionmentioning

confidence: 99%

“…Other in-phase and out-ofphase A-U-G and G-U-G codons are ignored, indicating that additional features within the mRNA sequence must effect discrimination [l, 21. Intercistronic regions are thought to be involved in the ribosomal recognition of starter triplets [ I , 2,5]. Over 20 regions, recognized by E. coli ribosomes, have now been sequenced [2].…”

Section: Discussionmentioning

confidence: 99%

“…One feature that may be important is the occurrence of bases at the 5' terminus of the RNAs which could conceivably complement with a unique sequence in the 3'-terminal portion of the 16-S RNA of the 30-S ribosomal subunit [l, 2,5]. However, inspection of nucleoside sequences around internal A-U-G codons also shows significant complementarity with the 3' end of 16-S RNA [6].…”

mentioning

confidence: 99%

“…synthesis [1,2,6,7] and also to code for methionine, valine and leucine residues respectively, when they occur in internal positions of mRNAs.…”

mentioning

confidence: 99%

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Synthesis of Biologically Active Portions of an Intercistronic Region by Use of a New 3′‐Phosphate Incorporation Method to Protect 3′‐OH and Their Binding to Ribosomes

Neilson

Gregoire

Fraser

et al. 1979

European Journal of Biochemistry

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To examine the influence of bases contiguous to a starter codon, a rapid means of assembling biologically active regions corresponding to portions, or to analogues of portions of intercistronic regions is desirable. To do this, a chemical method for the specific insertion of 3′‐monophosphate groups on to chemically synthesized trinucleotides, tetranucleotides and hexanucleotides of defined sequence bearing different bases at the 3′ terminus has been devised. The method involved phosphorylation of blocked oligoribonucleotides synthesized by a phosphotriester method. The deblocked oligoribonucleotides were phosphorylated at the 5′ end with T4‐induced polynucleotide kinase. The products of this kinase reaction served as“donors” in RNA ligase reactions. The [32P]pC‐A‐U‐A‐U‐Gp, [32P]pA‐U‐Gp, [32P]pU‐A‐A, [32P]pA‐G‐G‐Ap, [32P]pC‐U‐U‐Ap and [32P]pU‐C‐C‐Up “donors” were used to synthesize A‐G‐G‐A[32P]pC‐A‐U‐A‐U‐Gp, U‐C‐C‐U[32P]pC‐A‐U‐A‐U‐Gp, U‐A‐A[32P]pA‐U‐G, A‐U‐G[32P]pU‐A‐A, U‐A‐A‐G[32P]pA‐G‐G‐Ap, U‐C‐C‐U[32P]pC‐U‐U‐Ap and A‐U‐U‐C[32P]pU‐C‐C‐Up indicating that the method functions with all bases. A‐U‐Gp, PA‐U‐Gp and pC‐A‐U‐A‐U‐Gp were isolated free of reactants and, along with PA‐U‐G, were all shown to promote the formation of translational initiation complexes. A‐G‐G‐A[32P]pC‐A‐U‐A‐U‐Gp, which corresponds to the 5′‐terminal portion of the intracistronic region of the maturation protein of bacteriophage Qβ, bound more efficiently to Escherichia coli ribosomes than the U‐C‐C‐U[32P]pC‐A‐U‐A‐U‐Gp, [32P]pC‐A‐U‐A‐U‐Gp, [32P]pA‐U‐G‐U‐A‐A or [32P]pU‐A‐A‐A‐U‐G controls. The results suggested that the content and number of residues at the 5′ terminus attached to A‐U‐G affect binding of oligonucleotides to ribosomes; purine nucleosides appear to be more effective than pyrimidine nucleosides in this regard.

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Section: Discussionmentioning

confidence: 99%