RNA Recognition and Stress Granule Formation by  TIA Proteins

Waris, Saboora; Wilce, Matthew Charles James; Wilce, Jacqueline Anne

doi:10.3390/ijms151223377

Cited by 59 publications

(66 citation statements)

References 59 publications

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“…The proposed G4DNA signaling response to DNA oxidation reported here could be especially valuable to the cell by directly reporting the level of DNA damage to proteins that regulate translation (21,22,24). The fact that many cells can readily take up G4DNA indicates that this mechanism may also allow the cell to respond to its external environment as well as internal stress (67,72).…”

Section: Discussionmentioning

confidence: 88%

“…G4DNA from this endogenous source can participate in the assembly of stress granules, which are known to alter mRNA translation. Stress granules are cytoplasmic aggregates of mRNA and proteins that regulate mRNA translation and decay (21,22). They have been proposed to function as sites of mRNA triage (23), which govern the composition and function of ribonucleoprotein complexes in order to determine whether individual mRNAs are stored, degraded, or translation is reinitiated.…”

mentioning

confidence: 99%

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Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress

Byrd¹,

Zybailov

Maddukuri³

et al. 2016

Journal of Biological Chemistry

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Cells engage numerous signaling pathways in response to oxidative stress that together repair macromolecular damage or direct the cell toward apoptosis. As a result of DNA damage, mitochondrial DNA or nuclear DNA has been shown to enter the cytoplasm where it binds to "DNA sensors," which in turn initiate signaling cascades. Here we report data that support a novel signaling pathway in response to oxidative stress mediated by specific guanine-rich sequences that can fold into G-quadruplex DNA (G4DNA). In response to oxidative stress, we demonstrate that sequences capable of forming G4DNA appear at increasing levels in the cytoplasm and participate in assembly of stress granules. Identified proteins that bind to endogenous G4DNA in the cytoplasm are known to modulate mRNA translation and participate in stress granule formation. Consistent with these findings, stress granule formation is known to regulate mRNA translation during oxidative stress. We propose a signaling pathway whereby cells can rapidly respond to DNA damage caused by oxidative stress. Guanine-rich sequences that are excised from damaged genomic DNA are proposed to enter the cytoplasm where they can regulate translation through stress granule formation. This newly proposed role for G4DNA provides an additional molecular explanation for why such sequences are prevalent in the human genome.

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Section: Discussionmentioning

confidence: 88%

mentioning

confidence: 99%

Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress

Byrd¹,

Zybailov

Maddukuri³

et al. 2016

Journal of Biological Chemistry

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“…Cytoplasmic Tia-1 granules, so called ‘stress granules', represent stress-induced punctate structures that sequester stalled mRNAs and various proteins, which is generally thought to be part of an initially protective response34. Expression of WT CHCHD10 demonstrated nearly exclusive localization of TDP-43-tomato to the nucleus (Fig.…”

Section: Resultsmentioning

confidence: 99%

Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity

et al. 2017

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Although multiple CHCHD10 mutations are associated with the spectrum of familial and sporadic frontotemporal dementia–amyotrophic lateral sclerosis (FTD–ALS) diseases, neither the normal function of endogenous CHCHD10 nor its role in the pathological milieu (that is, TDP-43 pathology) of FTD/ALS have been investigated. In this study, we made a series of observations utilizing Caenorhabditis elegans models, mammalian cell lines, primary neurons and mouse brains, demonstrating that CHCHD10 normally exerts a protective role in mitochondrial and synaptic integrity as well as in the retention of nuclear TDP-43, whereas FTD/ALS-associated mutations (R15L and S59L) exhibit loss of function phenotypes in C. elegans genetic complementation assays and dominant negative activities in mammalian systems, resulting in mitochondrial/synaptic damage and cytoplasmic TDP-43 accumulation. As such, our results provide a pathological link between CHCHD10-associated mitochondrial/synaptic dysfunction and cytoplasmic TDP-43 inclusions.

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“…TIA-1/TIARs are composed of three N-terminal RNA recognition motifs (RRMs) that provide RNA/ DNA-binding specificity and a C-terminal prionrelated domain that confers self-aggregation, both of which are required for SG formation (Waris et al, 2014). In plants, RBP45/47 and OLIGOURIDYLATE BIND-ING PROTEIN1 (UBP1) are the triple RRM families most closely related to the animal TIA-1/TIARs.…”

Section: Mrna Sequestration In Sgs: a Transit Depot Between Translatimentioning

confidence: 99%

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

2017

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RNA Recognition and Stress Granule Formation by TIA Proteins

Cited by 59 publications

References 59 publications

Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress

Evidence That G-quadruplex DNA Accumulates in the Cytoplasm and Participates in Stress Granule Assembly in Response to Oxidative Stress

Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function

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