RNA-Protein Interactome at the Hepatitis E Virus Internal Ribosome Entry Site

Kumar, Shiv; Verma, Rohit; Saha, Sandhini; Agrahari, Ashish Kumar; Shukla, Shivangi; Singh, Oinam N; Berry, Umang; Maiti, Tushar Kanti; Asthana, Shailendra; Ranjith-Kumar, C. T.; Surjit, Milan

doi:10.1128/spectrum.02827-22

Microbiol Spectr

2023

DOI: 10.1128/spectrum.02827-22

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RNA-Protein Interactome at the Hepatitis E Virus Internal Ribosome Entry Site

Shiv Kumar

Rohit Verma

Sandhini Saha

et al.

Abstract: Protein synthesis is a fundamental process for survival and proliferation of all living organisms. The majority of cellular proteins are produced through cap-dependent translation.

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2023

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Virus–Host Protein Interaction Network of the Hepatitis E Virus ORF2-4 by Mammalian Two-Hybrid Assays

Corneillie,

Lemmens,

Weening

et al. 2023

Viruses

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Throughout their life cycle, viruses interact with cellular host factors, thereby influencing propagation, host range, cell tropism and pathogenesis. The hepatitis E virus (HEV) is an underestimated RNA virus in which knowledge of the virus–host interaction network to date is limited. Here, two related high-throughput mammalian two-hybrid approaches (MAPPIT and KISS) were used to screen for HEV-interacting host proteins. Promising hits were examined on protein function, involved pathway(s), and their relation to other viruses. We identified 37 ORF2 hits, 187 for ORF3 and 91 for ORF4. Several hits had functions in the life cycle of distinct viruses. We focused on SHARPIN and RNF5 as candidate hits for ORF3, as they are involved in the RLR-MAVS pathway and interferon (IFN) induction during viral infections. Knocking out (KO) SHARPIN and RNF5 resulted in a different IFN response upon ORF3 transfection, compared to wild-type cells. Moreover, infection was increased in SHARPIN KO cells and decreased in RNF5 KO cells. In conclusion, MAPPIT and KISS are valuable tools to study virus–host interactions, providing insights into the poorly understood HEV life cycle. We further provide evidence for two identified hits as new host factors in the HEV life cycle.

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Virus–Host Protein Interaction Network of the Hepatitis E Virus ORF2-4 by Mammalian Two-Hybrid Assays

Corneillie,

Lemmens,

Weening

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

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RNA-Protein Interactome at the Hepatitis E Virus Internal Ribosome Entry Site

Abstract: Protein synthesis is a fundamental process for survival and proliferation of all living organisms. The majority of cellular proteins are produced through cap-dependent translation.

Cited by 1 publication

References 80 publications

Virus–Host Protein Interaction Network of the Hepatitis E Virus ORF2-4 by Mammalian Two-Hybrid Assays

Virus–Host Protein Interaction Network of the Hepatitis E Virus ORF2-4 by Mammalian Two-Hybrid Assays

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