RNA processing in plant mitochondria is independent of transcription

Hinrichsen, Inga; Bolle, Nina; Paun, Linda; Kempken, Frank

doi:10.1007/s11103-009-9498-6

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…However, other maturation processes such as RNA trimming seem also to affect RNA editing of mRNAs and tRNAs (37,38). The possibility to introduce in vitro transcribed RNA into mitochondria (39) may help to answer some of the questions raised in this study, under conditions independent of the gene expression pathway.…”

Section: Discussionmentioning

confidence: 99%

Intron RNA editing is essential for splicing in plant mitochondria

Castandet

Choury

Bégu

et al. 2010

Nucleic Acids Research

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Most plant mitochondria messenger RNAs (mRNAs) undergo editing through C-to-U conversions located mainly in exon sequences. However, some RNA editing events are found in non-coding regions at critical positions in the predicted secondary and tertiary structures of introns, suggesting that RNA editing could be important for splicing. Here, we studied the relationships between editing and splicing of the mRNA encoding the ribosomal protein S10 (rps10), which has a group II intron and five editing sites. Two of them, C2 and C3, predicted to stabilize the folded structure of the intron necessary for splicing, were studied by using rps10 mutants introduced into isolated potato mitochondria by electroporation. While mutations of C2 involved in EBS2/IBS2 interactions did not affect splicing, probably by the presence of an alternative EBS2′ region in domain I of the intron, the edition of site C3 turned out to be critical for rps10 mRNA splicing; only the edited (U) form of the transcript was processed. Interestingly, RNA editing was strongly reduced in transcripts from two different intronless genes, rps10 from potato and cox2 from wheat, suggesting that efficient RNA processing may require a close interaction of factors engaged in different maturation processes. This is the first report linking editing and splicing in conditions close to the in vivo situation.

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Section: Discussionmentioning

confidence: 99%

Intron RNA editing is essential for splicing in plant mitochondria

Castandet

Choury

Bégu

et al. 2010

Nucleic Acids Research

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“…Introduction and subsequent transcription of DNA has been established via electroporation on mammalian (8), trypanosomatid (9) and plant isolated mitochondria (10–12). Electroporation of mRNAs into isolated plant mitochondria has been described and has enabled the study of the processing of mRNA precursors (13). Direct DNA uptake and its subsequent expression have also been obtained with plant, yeast and mammalian mitochondria (14–17).…”

Section: Introductionmentioning

confidence: 99%

A protein shuttle system to target RNA into mitochondria

Sieber¹,

Placido²,

Farouk-Ameqrane³

et al. 2011

Nucleic Acids Research

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Mitochondria play a key role in essential cellular functions. A deeper understanding of mitochondrial molecular processes is hampered by the difficulty of incorporating foreign nucleic acids into organelles. Mitochondria of most eukaryotic species import cytosolic tRNAs. Based on this natural process, we describe here a powerful shuttle system to internalize several types of RNAs into isolated mitochondria. We demonstrate that this tool is useful to investigate tRNA processing or mRNA editing in plant mitochondria. Furthermore, we show that the same strategy can be used to address both tRNA and mRNA to isolated mammalian mitochondria. We anticipate our novel approach to be the starting point for various studies on mitochondrial processes. Finally, our study provides new insights into the mechanism of RNA import into mitochondria.

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“…Several studies employing either in vitro or in organello RNA editing systems have provided important evidence on the process of mitochondrial and plastid RNA editing [23,26–28]. Even more relevant, several pentatricopeptide repeat proteins of the PLS subfamily are essential for editing of specific RNA editing sites [4–8].…”

Section: Discussionmentioning

confidence: 99%

“…These systems were successfully employed to analyze mitochondrial mRNA editing and the ability of plant mitochondria to recognize and correctly process RNAs from different taxa [23–27]. In addition, we were able to demonstrate complete in organello editing and splicing of in vitro transcribed cox2 mRNA [28]. …”

Section: Introductionmentioning

confidence: 99%

Deletions in cox2 mRNA Result in Loss of Splicing and RNA Editing and Gain of Novel RNA Editing Sites

et al. 2013

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As previously demonstrated, the maize cox2 RNA is fully edited in cauliflower mitochondria. Use of constructs with a deleted cox2 intron, however, led to a loss of RNA editing at almost all editing sites, with only a few sites still partially edited. Likewise, one deletion in exon 1 and three in exon 2 abolish RNA editing at all cox2 sites analyzed. Furthermore, intron splicing is abolished using these deletions. Mutation of a cytosine residue, which is normally edited and localized directly adjacent to the intron, to thymidine did not result in restoration of splicing, indicating that the loss of splicing was not due to loss of RNA editing. One deletion in exon 2 did not lead to loss of splicing. Instead, most editing sites were found to be edited, only three were not edited. Unexpectedly, we observed additional RNA editing events at new sites. Thus it appears that deletions in the cox2 RNA sequence can have a strong effect on RNA processing, leading to loss of splicing, loss of editing at all sites, or even to a gain of new editing sites. As these effects are not limited to the vicinity of the respective deletions, but appear to be widespread or even affect all editing sites, they may not be explained by the loss of PPR binding sites. Instead, it appears that several parts of the cox2 transcript are required for proper RNA processing. This indicates the roles of the RNA sequence and structural elements in the recognition of the editing sites.

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RNA processing in plant mitochondria is independent of transcription

Cited by 14 publications

References 23 publications

Intron RNA editing is essential for splicing in plant mitochondria

Intron RNA editing is essential for splicing in plant mitochondria

A protein shuttle system to target RNA into mitochondria

Deletions in cox2 mRNA Result in Loss of Splicing and RNA Editing and Gain of Novel RNA Editing Sites

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