RNA Processing Factor 7 and Polynucleotide Phosphorylase Are Necessary for Processing and Stability of nad2 mRNA in Arabidopsis Mitochondria

Stoll, Birgit; Zendler, Daniel; Binder, Stefan

doi:10.4161/rna.29781

Cited by 18 publications

(22 citation statements)

References 46 publications

(72 reference statements)

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“…In order to identify factors involved in this processing step, 5′ end polymorphisms observed among different Arabidopsis accessions were used to set up forward genetic screens (Forner et al ., ; Stoll et al ., ). This experimental strategy, as well as reverse genetic approaches, identified several RNA processing factors (RPFs) required for correct 5′ maturation of one or several transcripts (Jonietz et al ., , ; Hölzle et al ., ; Hauler et al ., ; Arnal et al ., ; Stoll et al ., , ). These factors are exclusively PPR proteins, which serve as sequence‐specific RNA‐binding proteins with no apparent enzymatic activity (Barkan et al ., ; Yagi et al ., ; Barkan and Small, ).…”

Section: Introductionmentioning

confidence: 99%

Two NYN domain containing putative nucleases are involved in transcript maturation in Arabidopsis mitochondria

Stoll

Binder

2016

The Plant Journal

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SUMMARYPlant mitochondrial transcripts frequently undergo maturation processes at their 5 0 ends. This almost completely enigmatic process requires the function of several proteins such as RNA processing factors, which are selectively involved in distinct 5 0 processing events. As RNA processing factors represent pentatricopeptide repeat proteins without apparent enzymatic function, it is hypothesized that a ribonuclease, most likely with endonucleolytic activity is involved in the 5 0 end maturation. We have now applied a reverse genetic approach to analyze the role of two potential mitochondrial nucleases, MNU1 and MNU2, in Arabidopsis thaliana. Both proteins contain several RNA-binding domains and NYN domains found in other endonucleases. A thorough analysis of various mitochondrial transcripts in MNU1 and MNU2 mutants revealed aberrant transcript pattern characterized by a decrease in mature RNA species often accompanied by an accumulation of larger, 5 0 extended precursor molecules. In addition, severely reduced amounts of nad9 mRNAs in the rpf2-1/mnu2-1 double mutant indicate a corporate function of RNA processing factor 2 and MNU2 in the maturation of these transcripts. However, the dramatic reduction of the nad9 mRNA is not reflected by the level of the corresponding Nad9 protein, which is found to be only moderately lowered. Collectively, our analysis strongly suggests a function of MNU1 and MNU2 in 5 0 processing of plant mitochondrial transcripts.

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Section: Introductionmentioning

confidence: 99%

Two NYN domain containing putative nucleases are involved in transcript maturation in Arabidopsis mitochondria

Stoll

Binder

2016

The Plant Journal

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“…Interestingly, there is natural variation among the abundance of the different nad4 precursor molecules in Arabidopsis accessions, which can be used to identify the factors involved in the stepwise 5′ maturation process. Multiple processing steps can also be assumed to be required for the generation of other mature mitochondrial mRNAs as indicated by the detection of several precursor molecules with different 5′ ends (Jonietz et al , Hauler et al , Stoll et al , ).…”

Section: Multiple Processing Events Might Be Necessary For the Generamentioning

confidence: 99%

“…By using this natural genetic variation in forward genetic approaches, several RPFs were identified (Jonietz et al , Hölzle et al , Stoll et al , ). Based on the results of these studies also reverse genetic strategies were successfully applied to identify and characterize further RPFs (Jonietz et al , Hauler et al ).…”

Section: Ppr Proteins Specifying 5′ Processingmentioning

confidence: 99%

“…Only the so far unidentified binding site of RPF7 is expected to be much shorter due to the comparatively small number of only eight repeats. However, in this case it remains unclear whether this protein alone can specify processing of the nad2 –122 5′ terminus (Stoll et al ). The RF‐like PPR proteins can have ecotype‐specific functions: for instance the function of RPF4 required for the generation of additional 5′ termini of the ccmB transcripts is restricted to Landsberg erecta (L er ) and several other ecotypes (Forner et al ). In addition, the function of RFL9, which cleaves within rps3‐rpl16 and in orf204a RNAs in Col and a few other accessions, is limited to a minor group of ecotypes (Arnal et al ).…”

Section: Two Coadapted Systems Specify 5′ Transcript End Formation Ofmentioning

confidence: 99%

“…However, rpf5‐1 seed germination capacity is found to be reduced and up to now this is the sole effect on ‘plant’ vitality observed in a natural or a T‐DNA mutant with an inactivated RPF (Hauler et al ). Similar to 26S rRNA, 5′ immature nad2 mRNAs are also less stable than its mature counterpart, however, the effect of impaired processing on nad2 transcript stability is much weaker than the one seen for 26S rRNA (Stoll et al ).…”

Section: Importance Of 5′ Processing Of Mitochondrial Rnasmentioning

confidence: 99%

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Maturation of 5′ ends of plant mitochondrial RNAs

Binder

Stoll

2016

Physiologia Plantarum

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The generation of mature RNAs, i.e. mRNAs, rRNAs or tRNAs, is a complex process in all genetic systems. RNA-internal processes such as splicing or RNA editing, but also posttranscriptional processes modulating 5' and 3' termini of transcripts, contribute to RNA maturation. In this article, we focus on the posttranscriptional formation of 5' termini of mitochondrial RNAs in seed plants, with particular emphasis on the model plant species Arabidopsis thaliana (Arabidopsis). We will summarize the progress made in recent studies of proteins involved in this process. In addition, we will evaluate whether 5' processing proceeds endo- or exo-nucleolytically. Despite the considerable progress made, many details of this process remain unsolved. In particular, it is still unclear why there is frequent 5' processing of many mRNAs although impaired processing does not interfere with mitochondrial function and plant fitness. Thus, the importance of 5' processing for plant mitochondria is still puzzling.

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Rna Metabolism and Transcript Regulation

Zmudjak

Zer

2017

Annual Plant Reviews, Volume 50

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RNA Processing Factor 7 and Polynucleotide Phosphorylase Are Necessary for Processing and Stability of nad2 mRNA in Arabidopsis Mitochondria

Cited by 18 publications

References 46 publications

Two NYN domain containing putative nucleases are involved in transcript maturation in Arabidopsis mitochondria

Two NYN domain containing putative nucleases are involved in transcript maturation in Arabidopsis mitochondria

Maturation of 5′ ends of plant mitochondrial RNAs

Rna Metabolism and Transcript Regulation

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