RNA Processing Activities of the Arabidopsis Argonaute Protein Family

Kim, Ki Wook; Eamens, Andrew L.; Waterhouse, Peter M.

doi:10.5772/22686

Cited by 2 publications

(3 citation statements)

References 87 publications

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“…The other tested interactions did not reveal obvious phenotypes. This was unexpected given the functional conservation of AGOs, and the ubiquitous expression of key members like AGO1 and AGO4 in most tissues including leaves, roots, and the vasculature (Martín-Merchán et al, 2023; Wook et al, 2011). The transcriptional response of DCL and AGO genes was mostly not correlated with infection phenotypes in respective mutants.…”

Section: Discussionmentioning

confidence: 99%

“…The members of clade I and clade III, AGO1 and AGO4, are ubiquitously expressed across tissues and during various developmental stages of A. thaliana (Jullien et al, 2022). AGO5 , AGO6, AGO7, AGO9 , and AGO10, members across all clades, exhibit specific expression patterns with spatial and temporal regulation during plant development (Mallory & Vaucheret, 2010; Wook et al, 2011). The expression of AGO2 and AGO3 is induced in response to diverse abiotic and biotic stresses (Martín-Merchán et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

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Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions

Ruf,

Thieron,

Nasfi

et al. 2024

Preprint

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RNA interference (RNAi) is a crucial mechanism that can contribute to immunity against infectious microbes through the action of DICER-LIKE (DCL) and ARGONAUTE (AGO) proteins. In the case of the fungal pathogenBotrytis cinereaand the oomyceteHyaloperonospora arabidopsidis, plant DCL and AGO proteins have proven roles as negative regulators of immunity, suggesting functional specialization of these proteins. To address this aspect in a broader taxonomic context, we characterized the colonization pattern of an informative set ofDCLandAGOloss-of-function mutants inArabidopsis thalianaupon infection with a panel of pathogenic microbes with different lifestyles, and a fungal mutualist. Our results revealed that AGO1 and AGO4 function as positive regulators of immunity to a bacterial and a fungal pathogen, respectively. Additionally, AGO2 and AGO10 positively modulated the colonization by a fungal mutualist. Therefore, analysing the role of RNAi across a broader range of plant-microbe interactions has identified previously unknown functions for AGO proteins. For some pathogen interactions, however, all tested mutants exhibited wild type-like infection phenotypes, suggesting that the roles of AGO and DCL proteins in these interactions may be more complex to elucidate.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions

Ruf,

Thieron,

Nasfi

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Thus, it could be seen that although many resistance genes were derived from wild varieties, the disease resistance of the hybrid varieties was significantly higher than that of the wild varieties after long-term natural and artificial selection (Gerechter-Amitai et al 1971;Li et al 2018). In recent years, AGO gene family members have been isolated and identified from many higher plants such as A. thaliana, apples, maize, and rice through bioinformatics analyses (Kapoor et al 2008;Kim et al 2011;Qian et al 2011;Xu et al 2016). RNA https://doi.org/10.17221/180/2020-PPS interference plays an important role in plant growth and development.…”

Section: Discussionmentioning

confidence: 99%

Use of gene family analysis to discover argonaut (AGO) genes for increasing the resistance of Tibetan hull-less barley to leaf stripe disease

Yao¹,

Wang²,

Yao³

et al. 2021

Plant Prot. Sci.

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Leaf stripe is a common, but major infectious disease of barley, severely affecting the yield and quality. However, only a few genes have been identified by conventional gene mapping. Gene family analysis has become a fast and efficient strategy for gene discovery. Studies demonstrated that Argonaute (AGO) proteins play an important role in plant disease resistance. Thus, we obtained nine HvAGO genes via mRNA sequencing before and after a Pyrenophora graminea infection of a disease-resistant variety "Kunlun 14" and a susceptible variety "Z1141". We analysed the physicochemical characteristics, gene structures, and motifs of the HvAGO gene sequences and found that these proteins were divided into four clusters by evolutionary distance. There was high consistency in the number of exons, size, and the number and type of motifs in the different clusters. Based on protein phylogenetics, they could be divided into three branches. A collinearity analysis of Tibetan hull-less barley and Arabidopsis thaliana, rice, and maize showed that four genes were collinear with respect to the other three species. The qRT-PCR showed the expression levels of HvAGO1, HvAGO2 and HvAGO4 were significantly increased after infection with Pyrenophora graminea. These three members of the AGO gene family are, thus, speculated to play an important role in barley leaf stripe resistance. The results provide reference for the application of HvAGO genes in the leaf stripe control and the exploration of disease resistance genes in other crops.

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RNA Processing Activities of the Arabidopsis Argonaute Protein Family

Cited by 2 publications

References 87 publications

Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions

Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions

Use of gene family analysis to discover argonaut (AGO) genes for increasing the resistance of Tibetan hull-less barley to leaf stripe disease

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