RNA precursor molecules and ribonucleases in E. coli

Altman, Sidney; Robertson, Hugh D.

doi:10.1007/bf01659941

Cited by 39 publications

(5 citation statements)

References 81 publications

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“…ipient, but Ade+ like the donor Since RNase III was implicated in the pro- FIG. 1. RNase III activity in various strains.…”

Section: Resultsmentioning

confidence: 99%

Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid

Apirion¹,

Watson²

1975

J Bacteriol

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Localization of a mutation affecting ribonuclease III activity (an enzyme specific for double-stranded ribonucleic acid) in Escherichia coli was attempted. By a series of matings and transduction experiments, the mutation rnc-105 was mapped near the nadB gene. In strains carrying this mutation, another mutation (ranA2074) was also found. Based on available data, their order on the E. coli chromosome appears to be tyrA, ranA, nadB, rnc, duction and matings were performed as described previously (4,11,12).Bacteriophage plating. Phage were plated as described previously (4).Assay of RNase III. RNase assays were done as described by Robertson et al. (14), Meyhack et al. (13), and Apirion and Watson (4).Nomenclature. We suggest that genes affecting RNase activities should be referred to as ma, rnb, etc., 317 on July 31, 2020 by guest

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“…ipient, but Ade+ like the donor Since RNase III was implicated in the pro- FIG. 1. RNase III activity in various strains.…”

Section: Resultsmentioning

confidence: 99%

Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid

Apirion¹,

Watson²

1975

J Bacteriol

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“…The fact that RNA fragments were removed during maturation raised several questions. As stated by Altman and Robertson in 1973 [13], “A major question about these molecules is that of the function of the “extra” segments”. Another question was how are these precursors processed to generate the mature tRNA and rRNA species.…”

Section: Rna Derived Fragmentsmentioning

confidence: 99%

The unmasking of ‘junk’ RNA reveals novel sRNAs: from processed RNA fragments to marooned riboswitches

Lay

Garsin

2016

Current Opinion in Microbiology

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While the notion that RNAs can function as regulators dates back to early molecular studies of gene regulation of the lac operon, it is only over the last decade that the ubiquity and diversity of regulatory RNAs are being realized. Advancements in high throughput sequencing and the adoption of these approaches to rapidly sequence genomes and transcriptomes and to examine gene expression and RNA binding protein specificity have revealed an ever-expanding RNA world. In this review, we focus on recent studies revealing that RNA fragments cleaved from larger coding or noncoding RNAs can have regulatory functions. Additionally, we discuss examples of riboswitches that function in trans as mRNA or protein-binding sRNAs, upending the traditional thinking that these are exclusively cis-acting elements.

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“…95% ethanol. It was further purified via its insoluble cetyltrimethyl ammonium salt as previously described [8]. Pure CPS was obtained by gel filtration on a column of Sephadex G-50 followed by ionexchange chromatography on a column of DEAE-Sephacel from which it was eluted as a discrete homogeneous fraction with approximately 0.4 M sodium chloride.Fermentor-grown cells.…”

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confidence: 99%

“…The cells were harvested by scrapping and then suspended in 1% (w/v) aqueous phenol solution. Capsular material was also isolated from the cells grown in a 28-1 fermentor (New Brunswick Scientific) as previously described [8] (yield z 3 g wet weight/l).…”

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confidence: 99%

Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 5

Altman

Brisson

Perry

1987

European Journal of Biochemistry

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The capsular polysaccharide of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5 (ATCC 33377) was found to be a linear type polysaccharide of a repeating disaccharide unit composed of 2-acetamido-2-deoxy-~-glucose and 3-deoxy-~-manno-2-octulosonic acid (dOclA). By composition analysis, methylation, partial hydrolysis and lH and I3C nuclear magnetic resonance studies, it was concluded that the capsular polysaccharide is a high-molecular-mass unbranched polymer having the structure :Haemophilus (Actinobacillus) pleuropneumoniae is the causative agent of porcine Haemophilus pleuropneumonia, a major respiratory disease in swine throughout the world.Previous investigations have related H . pleuropneumoniae serotype to capsular polysaccharides (CPS) and capsular structure to virulence [l]. In addition, clinical and experimental evidence indicates the possible involvement of component lipopolysaccharides in the pathogenesis of porcine pleuropneumonia [2].Various serotyping schemes and serological assays have been used to identify potential carrier animals [3,4]. However, crude antigens used in these assays are poorly defined and provide little information about the chemical identity of these biologically important antigens.To understand the pathogenesis, serology, and immunobiology involved in the infections, we have undertaken the structural chemical characterization of CPS and lipopolysaccharide components of the ten known serotypes Recently, the structures of the CPS and 0-specific polysaccharides of the S-form lipopolysaccharide from H . pleuropneumoniae serotypes 1, 2 and 3 have been determined H. pleuropneumoniae serotype 5 isolations are common in Western Canada and the United States where they represent 58% and 54% of isolates respectively [ l l , 121. This paper records the structural analysis of the CPS from H . pleuropneumoniae serotype 5.[5 -71.[8 -lo]. MATERIALS AND METHODS MaterialsHaemophilus pleuropneumoniae serotype 5 (ATCC 33377) was obtained from the Veterinary Infectious Disease OrgaCorrespondence to M. B. Perry, Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada KIA OR6Abbreviations. CPS, capsular polysaccharide; COSY, correlated spectroscopy; relay COSY, relayed coherence transfer spectroscopy; 2D, two-dimensional; FID, free induction decay; DEPT, distortionless enhancement hy polarization transfer; CHORTLE, carbon-hydrogen correlations from one-dimensional polarization transfer spectra by least-square analysis; tcA, retention time relative to ~-glucitol hexaacetate; tcM, retention time relative to 1,S-di-Oacetyl-2,3,4,6-tetra-O-methyl-~-glucitol. nization, Sask. The majority of the studies were done with cells grown on plates, containing Difco PPLO broth (36 g/ 1) supplemented with filter-sterilized horse serum (5%, w/v) (Bockneck Labs), NAD and D-glucose (O.lYn, w/v), for 17 h and 37 "C in candlejars. The cells were harvested by scrapping and then suspended in 1% (w/v) aqueous phenol solution. Capsular material was also isolated from the cells grown in a ...

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RNA precursor molecules and ribonucleases in E. coli

Cited by 39 publications

References 81 publications

Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid

Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid

The unmasking of ‘junk’ RNA reveals novel sRNAs: from processed RNA fragments to marooned riboswitches

Structure of the capsular polysaccharide of Haemophilus pleuropneumoniae serotype 5

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