“…For example, no Ile-tRNA or very few Arg-tRNA but a few Asp-tRNA molecules were detected; however, Cys, Leu, Phe, Try, Trp, and Ser tRNAs were predominantly present in the phloem sap ( Zhang et al, 2009 ). These tRNAs have been predicted to serve as long-distance signals ( Zhang et al, 2009 , 2016 ; Lezzhov et al, 2019 ; Wang et al, 2021 ), suggesting that tRNA or tRNA-like structure could be an important factor, if not the determinant for systemic RNA movement. Indeed, in the ViGE systems, like the FT mRNA/102 nt- FT RNA element, Met, Gly, and Ile tRNAs were also found to improve transgenerational gene editing when they were fused to sgRNA ( Ellison et al, 2020 ).…”