RNA-Mediated Virus Resistance in Transgenic Plants: Exploitation of a Cellular Pathway Possibly Involved in RNA Degradation

Dougherty, William G.; Lindbo, John A.; Smith, Holly A.; Parks, T. Dawn; Swaney, S; Proebsting, William M.

doi:10.1094/mpmi-7-0554

Cited by 174 publications

(66 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The phenotypes observed in our P1 and P3 lines coincide with the two resistance phenotypes that have been associated with PTGS in other systems (Dougherty et al, 1994 ;Goodwin et al, 1996). These are the ' immunity ' or ' resistant ' phenotype (R ; no symptoms and no accumulation of virus) and the ' recovery ' phenotype (RC ; systemic symptoms that gradually disappear in newly developed leaves some time after inoculation).…”

Section: Discussionsupporting

confidence: 85%

“…CP-mediated resistance has been described for many potyvirus-host combinations (Fitchen & Beachy, 1993 ;Hackland et al, 1994). Also, the expression of untranslatable versions of the CP gene of a potyvirus led to the first demonstration of RNA-mediated resistance in plant viruses (Lindbo et al, 1993) and has been extensively studied (Dougherty et al, 1994 ;Cassidy & Nelson, 1995 ;Goodwin et al, 1996). The ability of some potyvirus non-structural genes to induce resistance is beginning to be investigated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus.

Moreno

Bernal

Jiménez

et al. 1998

Journal of General Virology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus.

Moreno

Bernal

Jiménez

et al. 1998

Journal of General Virology

View full text Add to dashboard Cite

“…We have previously suggested that TEV transgenic resistance may be mediated by a cellular pathway normally involved in the elimination or down-regulation of aberrant or overexpressed mRNAs (Lindbo et al, 1993b;Dougherty et al, 1994). Consistent with these observations, transgenic plants resistant to PVY usually have multiple PVY transgenes that appear to be highly transcribed, yet accumulate low levels of the transgene transcript.…”

Section: Discussionmentioning

confidence: 67%

“…Estlmation of Transcription Rates: Nuclear Run-Off Assays lsoation of nuclei from transgenic plant tissue, in vitro labeling of run-on transcripts, and blot hybridization were as originally described by Cox and Goldberg (1988) and modified by Dougherty et al (1994).…”

Section: Analysls Of Genomic Dna Ln Lkansgenic Plantsmentioning

confidence: 99%

See 1 more Smart Citation

Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs.

et al. 1994

View full text Add to dashboard Cite

Haploid leaf tissue of tobacco cultivars K326 and K149 was transformed with severa1 transgenes containing cDNA of the potato virus Y (PVY) coat protein (CP) open reading frame (ORF). The various transgenes containing the PVY CP ORF sequence produced (1) the expected mRNA and CP product, (2) an mRNA rendered untranslatable by introduction of a stop codon immediately after the initiation codon, or (3) an antisense RNA that was untranslatable as a result of the incorrect orientation of the PVY CP ORF behind the transcriptional promoter. Homozygous doubled haploid (DH) (diploid) plants were generated, and selfed progeny from these plants were examined. Reslstance was virus specific, functioning only against PVY. An inverse correlation between transgene-derived PVY transcript steady state levels and resistance was generally noted with lines expressing the untranslatable sense version of the PVY CP ORF. A collection of DH lines, derived from a single transformation event of a common haploid plant and isogenic for the PVY transgenes expressing untranslatable sense RNA, displayed different levels of PVY resistance. Lines with actively transcribed, methylated transgene sequences had low steady state levels of transgene transcript and a virus-resistant phenotype. These results are discussed within the context of sense suppression in plants.

show abstract

The Use of Transgenic Papaya to Control Papaya Ringspot Virus in Hawaii, and the Transfer of this Technology to Other Countries

Fermín

2004

Handbook of Plant Biotechnology

View full text Add to dashboard Cite

A handful of virus‐resistant transgenic plants have been commercialised in the United States. This communication does not dwell on discussing possible reasons for the lack of other commercial virus‐resistant transgenic plants. Rather it focuses on an analysis of the events involved in development and commercialisation of transgenic papaya that is currently grown in Hawaii to control Papaya ringspot virus , on the impact of the varieties on the Hawaii papaya industry, on the current status of the Hawaiian transgenic papaya in United States, Canada, and Japan, and on our efforts to transfer this technology to other countries. This paper primarily deals with the rationale and motivations for the commercialisation of transgenic papaya, and to a lesser degree on the technical development of the transgenic papaya.

show abstract

RNA-Mediated Virus Resistance in Transgenic Plants: Exploitation of a Cellular Pathway Possibly Involved in RNA Degradation

Cited by 174 publications

References 0 publications

Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus.

Resistance in plants transformed with the P1 or P3 gene of tobacco vein mottling potyvirus.

Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation, and fate of nonessential RNAs.

The Use of Transgenic Papaya to Control Papaya Ringspot Virus in Hawaii, and the Transfer of this Technology to Other Countries

Contact Info

Product

Resources

About