RNA-Mediated Resistance with Nonstructural Genes from the Tobacco Etch Virus Genome

Swaney, S; Powers, H. R.; Goodwin, Joel B.; Rosales, Laura Silva; Dougherty, William G.

doi:10.1094/mpmi-8-1004

Cited by 46 publications

(27 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tobacco plants inoculated with the PVX:GUS: CP amplicon accumulated virus-derived sequences at a much lower than expected level and showed no symptoms of PVX infection. The unexpected phenotype of PVX:GUS:CP infection was similar to those reported for other homology-dependent resistance studies of plants expressing viral genome sequences (Swaney et al, 1995).…”

Section: Yesterday's Rna Silencing In Plants-so Many Curious Findings!supporting

confidence: 85%

RNA Silencing in Plants: Yesterday, Today, and Tomorrow

Eamens

Wang²,

Smith³

et al. 2008

Plant Physiology

278

202

View full text Add to dashboard Cite

Section: Yesterday's Rna Silencing In Plants-so Many Curious Findings!supporting

confidence: 85%

RNA Silencing in Plants: Yesterday, Today, and Tomorrow

Eamens

Wang²,

Smith³

et al. 2008

Plant Physiology

278

202

View full text Add to dashboard Cite

“…The steady state level of the transgene transcript was estimated to decrease two-to fourfold with the induction of the highly resistant state ( Figure 3B). A similar but often larger (approximately six-to 15-fold) decrease in transgene transcript steady state level has been noted with other virus transgenes that rapidly induce the recovery phenotype (Lindbo et al, 1993;Dougherty et al, 1994;Swaney et al, 1995). The difference observed for FL-44.4, a TEV CP-producing line, is M2-fold (Lindbo et al, 1993) and is shown in lanes 3 and 4 of Figure 3B.…”

Section: Rna Analysissupporting

confidence: 73%

“…The transgene-derived RNA may be either translatable or untranslatable, indicating that the translation product of the transcript is not involved in eliciting resistance. Plants displaying this virus-resistant phenotype transcribe the transgene(s) at a high rate yet accumulate the transgene transcript at low levels Smith et al, 1994;Mueller et al, 1995;Swaney et al, 1995). Plants with these characteristics do not permit the cytoplasmically localized virus to replicate to a detectable level.…”

Section: Introductionmentioning

confidence: 99%

Genetic and Biochemical Dissection of Transgenic RNA-Mediated Virus Resistance

Goodwin¹,

Chapman²,

Swaney³

et al. 1996

The Plant Cell

Self Cite

View full text Add to dashboard Cite

“…This phenomenon was first observed and intensively studied in plant systems (see reference 64 for a review), where it has been associated with several processes, including cosuppression (44), repeat induced gene silencing (70), RNA-mediated resistance (35,58), or homology-dependent gene silencing (43). Similar mechanisms were later discovered in other organisms, including quelling in filamentous fungus Neurospora crassa (9) and RNA interference (RNAi) in Caenorhabditis elegans (18) and Drosophila melanogaster (30).…”

mentioning

confidence: 85%

The Coat Protein of Turnip Crinkle Virus Suppresses Posttranscriptional Gene Silencing at an Early Initiation Step

Ren

Morris

2003

J Virol

284

269

View full text Add to dashboard Cite

Posttranscriptional gene silencing (PTGS), or RNA silencing, is a sequence-specific RNA degradation process that targets foreign RNA, including viral and transposon RNA for destruction. Several RNA plant viruses have been shown to encode suppressors of PTGS in order to survive this host defense. We report here that the coat protein (CP) of Turnip crinkle virus (TCV) strongly suppresses PTGS. The Agrobacterium infiltration system was used to demonstrate that TCV CP suppressed the local PTGS as strongly as several previously reported virus-coded suppressors and that the action of TCV CP eliminated the small interfering RNAs associated with PTGS. We have also shown that the TCV CP must be present at the time of silencing initiation to be an effective suppressor. TCV CP was able to suppress PTGS induced by sense, antisense, and doublestranded RNAs, and it prevented systemic silencing. These data suggest that TCV CP functions to suppress RNA silencing at an early initiation step, likely by interfering the function of the Dicer-like RNase in plants.Posttranscriptional gene silencing (PTGS) is a sequencespecific RNA degradation process that leads to the elimination of the targeted RNA and loss of the function(s) encoded by the targeted RNA (1,6,62,69). This phenomenon was first observed and intensively studied in plant systems (see reference 64 for a review), where it has been associated with several processes, including cosuppression (44), repeat induced gene silencing (70), RNA-mediated resistance (35, 58), or homology-dependent gene silencing (43). Similar mechanisms were later discovered in other organisms, including quelling in filamentous fungus Neurospora crassa (9) and RNA interference (RNAi) in Caenorhabditis elegans (18) and Drosophila melanogaster (30). Recent research has revealed that all of these different phenomena have many common features and are now considered to be manifestations of an RNA-targeting pathway, whose natural functions include protecting hosts from invading viral RNAs and transposons (see references 45, 54, and 72 for reviews). RNA silencing has been proposed as a more general term to describe these related processes (1).Initiation and maintenance stages have been identified as distinct phases of the PTGS or RNA silencing process (10,50,65). In the initiation stage, the invading RNA triggers a pathway that results in its being degraded into a small RNA species of discrete size (21 to 25 nucleotides [nt]) called small interfering RNAs (siRNAs) that function as a guide for further degradation in the maintenance stage (22, 71). The most potent initiator of PTGS is thought to be double-stranded RNA (dsRNA) (8,18,30,68), although single-stranded RNA (ssRNA), both sense and antisense orientations, or even DNA trigger RNA silencing (15,65,66). ssRNA is most likely converted to a double-stranded form with the help of a host RNAdependent RNA polymerase (RdRP) in order to be effective (9,11,42,52,57). The dsRNA initiators are then degraded by an RNase III-like RNase (e.g., Dicer in Drosophila [3])...

show abstract

RNA-Mediated Resistance with Nonstructural Genes from the Tobacco Etch Virus Genome

Cited by 46 publications

References 0 publications

RNA Silencing in Plants: Yesterday, Today, and Tomorrow

RNA Silencing in Plants: Yesterday, Today, and Tomorrow

Genetic and Biochemical Dissection of Transgenic RNA-Mediated Virus Resistance

The Coat Protein of Turnip Crinkle Virus Suppresses Posttranscriptional Gene Silencing at an Early Initiation Step

Contact Info

Product

Resources

About