RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae

Peschek, Nikolai; Herzog, Rudolf; Singh, Praveen; Sprenger, Marcel; Fröhlich, Kathrin S.; Schröger, Luise; Bramkamp, Marc; Drescher, Knut; Papenfort, Kai

doi:10.1038/s41467-020-19890-8

Cited by 26 publications

(17 citation statements)

References 60 publications

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“…Additionally, it is also worth mentioning that spherical H. pylori forms tend to self-aggregate and create biofilms [ 72 , 85 ], so it is difficult to distinguish planktonic and biofilm forms of this bacterium. According to the authors of this review, it seems that the processes of morphological transformation and biofilm formation of H. pylori are closely related and may induce one another, as observed in other microorganisms [ 86 , 87 , 88 , 89 ]. Spherical forms may stimulate biofilm formation through a high autoaggregation capacity, while biofilm may intensify the transition of H. pylori to spherical forms through the presence of niches with suboptimal growth conditions (e.g., reduced concentrations of nutrients or respiratory gases).…”

Section: Phenotypic Variability As a Modulator Of H Pylmentioning

confidence: 99%

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

et al. 2020

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Helicobacter pylori is a bacterium that is capable of colonizing a host for many years, often for a lifetime. The survival in the gastric environment is enabled by the production of numerous virulence factors conditioning adhesion to the mucosa surface, acquisition of nutrients, and neutralization of the immune system activity. It is increasingly recognized, however, that the adaptive mechanisms of H. pylori in the stomach may also be linked to the ability of this pathogen to form biofilms. Initially, biofilms produced by H. pylori were strongly associated by scientists with water distribution systems and considered as a survival mechanism outside the host and a source of fecal-oral infections. In the course of the last 20 years, however, this trend has changed and now the most attention is focused on the biomedical aspect of this structure and its potential contribution to the therapeutic difficulties of H. pylori. Taking into account this fact, the aim of the current review is to discuss the phenomenon of H. pylori biofilm formation and present this mechanism as a resultant of the virulence and adaptive responses of H. pylori, including morphological transformation, membrane vesicles secretion, matrix production, efflux pump activity, and intermicrobial communication. These mechanisms will be considered in the context of transcriptomic and proteomic changes in H. pylori biofilms and their modulating effect on the development of this complex structure.

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Section: Phenotypic Variability As a Modulator Of H Pylmentioning

confidence: 99%

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

et al. 2020

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“…VxrAB (also known as WigKR) was first identified for its implication in colonization in the infant mouse model and T6SS regulation (34). VxrAB has already been described to regulate other systems implicated in antimicrobial resistance, such as biofilm formation, motility and cell shape maintenance and homeostasis in the presence of cell wall targeting antimicrobials (30, 66, 77). In the later, they demonstrated that the expression of vxrAB is increased by antibiotic-induced cell wall damage, and that VxrAB regulates the entire cell wall synthesis pathway, leading to V. cholerae’s tolerance to antibiotics (66).…”

Section: Discussionmentioning

confidence: 99%

Comprehension of Antimicrobial Peptides Modulation of the Type VI Secretion System inVibrio cholerae

Mathieu-Denoncourt

Duperthuy

2022

Preprint

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The Type VI secretion System (T6SS) is a versatile weapon used by bacteria for virulence, resistance to grazing and competition with other bacteria. We previously demonstrated that the role of the T6SS in interbacterial competition and in resistance to grazing is enhanced in Vibrio cholerae in the presence of subinhibitory concentrations of polymyxin B (PmB). In this study, we performed a global quantitative proteomic analysis by liquid chromatography coupled to mass spectrometry and a transcriptomic analysis by quantitative PCR of the T6SS known regulators in V. cholerae grown with and without PmB. The proteome of V. cholerae is greatly modified in the presence of PmB at subinhibitory concentrations with more than 39 % of the identified cellular proteins displaying a difference in their abundance, including T6SS-related proteins (Hcp, VasC, TsaB and ClpV). We identified a regulator whose abundance and expression are increased in the presence of PmB, vxrB, the response regulator of the two-component system VxrAB. In a vxrAB deficient mutant, the expression of hcp measured by quantitative PCR, although globally reduced, was not modified in the presence of PmB, confirming its role in hcp upregulation with PmB. The upregulation of the T6SS in the presence of PmB appears to be, at least in part, due to the two-component system VxrAB.

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“…This form of regulation promotes the degradation of dsRNA by the action of RNase III (a dsRNA-specific endonuclease) and the production of small dsRNAs [ 130 ], or by blocking or inducing translation [ 131 ]. This kind of RNA regulation controls a wide range of activities inside cells, such as riboswitch elements, toxin–antitoxin modules, mobility, DNA repair, metabolism, gene regulation, cell shape, and biofilm formation [ 101 , 123 – 125 , 129 , 132 ]. It is also used by plasmids, TEs, and phages for self-regulation [ 100 , 101 , 128 ].…”

Section: Introductionmentioning

confidence: 99%

The origin of RNA interference: Adaptive or neutral evolution?

et al. 2022

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The origin of RNA interference (RNAi) is usually explained by a defense-based hypothesis, in which RNAi evolved as a defense against transposable elements (TEs) and RNA viruses and was already present in the last eukaryotic common ancestor (LECA). However, since RNA antisense regulation and double-stranded RNAs (dsRNAs) are ancient and widespread phenomena, the origin of defensive RNAi should have occurred in parallel with its regulative functions to avoid imbalances in gene regulation. Thus, we propose a neutral evolutionary hypothesis for the origin of RNAi in which qualitative system drift from a prokaryotic antisense RNA gene regulation mechanism leads to the formation of RNAi through constructive neutral evolution (CNE). We argue that RNAi was already present in the ancestor of LECA before the need for a new defense system arose and that its presence helped to shape eukaryotic genomic architecture and stability.

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RNA-mediated control of cell shape modulates antibiotic resistance in Vibrio cholerae

Cited by 26 publications

References 60 publications

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

Biofilm Formation as a Complex Result of Virulence and Adaptive Responses of Helicobacter pylori

Comprehension of Antimicrobial Peptides Modulation of the Type VI Secretion System inVibrio cholerae

The origin of RNA interference: Adaptive or neutral evolution?

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