RNA interference tools for the western flower thrips, Frankliniella occidentalis

Badillo-Vargas, Ismael E.; Rotenberg, Dorith; Schneweis, Brandi A.; Whitfield, Anna E.

doi:10.1016/j.jinsphys.2015.03.009

Cited by 66 publications

(64 citation statements)

References 79 publications

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“…Similar dsRNA- VATPase silencing phenotypes have been described across multiple experimental systems, e.g. whiteflies, western corn rootworms, western flower thrips, Colorado potato beetles, fruit flies, flour beetles, pea aphids, and tobacco hornworms [12,20–22,38,39]. They confirm the essential role of the VATPase pump in proton transport across cellular membranes of eukaryotic organisms [19].…”

Section: Discussionsupporting

confidence: 65%

RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing

et al. 2017

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RNA interference (RNAi) can be used for the protection against agricultural pests through the silencing of genes required for pest fitness. To assess the potential of RNAi approaches in the two-spotted spider mite, Tetranychus urticae, we compared 5 methods for the delivery of double-stranded RNA (dsRNA). These methods include mite feeding on either (i) leaves floating on a dsRNA solution, (ii) dsRNA-expressing plants, (iii) artificial diet supplemented with dsRNA, or (iv) dsRNA-coated leaves, and (v) mite soaking in a dsRNA solution. In all cases, the gene targeted for method validation was the Vacuolar-type H+-ATPase (TuVATPase), encoding a constitutively expressed ATP-driven proton pump located in the membrane. Down-regulation of TuVATPase increased mortality and/or reduced fecundity in all methods, but with variable efficiency. The most efficient methods for dsRNA delivery were direct soaking of mites in the dsRNA solution and mite feeding on dsRNA-coated leaves that mimics dsRNA application as a sprayable pesticide. Both resulted in a dark-body phenotype not observed in mites treated with a control dsRNA. Although with lower efficiency, dsRNA designed for TuVATPase silencing and expressed in transgenic Arabidopsis plants impacted the fitness of mites feeding on these plants. RNAi may thus be a valuable strategy to control spider mite populations, either as a sprayable pesticide or through transgenic crops. This comparative methodological study focusing on the induction of RNAi-based gene silencing in T. urticae paves the way for reverse genetics approaches in this model chelicerate system and prepares large-scale systematic RNAi screens as a first step towards the development of specific RNA-based pesticides. Such alternative molecules may help control spider mites that cause significant damages to crops and ornamental plant species, as well as other chelicerates detrimental to agriculture and health.

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Section: Discussionsupporting

confidence: 65%

RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing

et al. 2017

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“…9 Since then, F. occidentalis has spread rapidly throughout China to become one of the major pests of vegetable and floriculture crops. 2,10 Although many approaches have been developed for the management of thrips, 11 -13 control efforts against F. occidentalis continue to be heavily reliant on the application of insecticides, especially in virus-sensitive crops.…”

Section: Introductionmentioning

confidence: 99%

Field-evolved resistance to insecticides in the invasive western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in China

et al. 2016

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“…These results are consistent with previous results on RNAi induced mortality in bed bugs with vATPase-A , brahma, and vitellogenin (Moriyama et al 2016; Basnet and Kamble 2017, 2018). In other insects, such as Frankliniella occidentalis Pergande (Badillo-Vargas et al 2015), significant mortality was observed as early as 6 d post injection. Rangasamy and Siegfried (2012) observed 100% mortality in 14 d after the knockdown of vATPase in D. v. virgifera .…”

Section: Discussionmentioning

confidence: 99%

RNA Interference of the Muscle Actin Gene in Bed Bugs: Exploring Injection Versus Topical Application for dsRNA Delivery

Basnet

Kamble

2018

Journal of Insect Science

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Bed bugs are one the most troublesome household pests that feed primarily on human blood. RNA interference (RNAi) is currently being pursued as a potential tool for insect population management and has shown efficacy against some phytophagous insects. We evaluated the different techniques to deliver dsRNA specific to bed bug muscle actin (dsactin) into bed bugs. Initially, stability of dsRNA in human blood was studied to evaluate the feasibility of feeding method. Adult bed bugs were injected with dsRNA between last thoracic segment and first abdominal segment on the ventral side, with a dose of 0.2 µg dsactin per insect. In addition to injection, dsactin was mixed in acetone and treated topically in the abdomens of fifth stage nymphs. We found the quick degradation of dsRNA in blood. Injection of dsactin caused significant depletion of actin transcripts and substantial reduction in oviposition and lethality in female adults. Topically treated dsRNA in fifth stage nymphs had no effect on actin mRNA expression and survival. Our results demonstrated that injection is a reliable method of dsRNA delivery into bed bugs while topical treatment was not successful. This research provides an understanding on effective delivery methods of dsRNA into bed bugs for functional genomics research and feasibility of the RNAi based molecules for pest management purposes.

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RNA interference tools for the western flower thrips, Frankliniella occidentalis

Cited by 66 publications

References 79 publications

RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing

RNAi-based reverse genetics in the chelicerate model Tetranychus urticae: A comparative analysis of five methods for gene silencing

Field-evolved resistance to insecticides in the invasive western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) in China

RNA Interference of the Muscle Actin Gene in Bed Bugs: Exploring Injection Versus Topical Application for dsRNA Delivery

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