RNA interference suppressing PLCE1 gene expression decreases invasive power of human bladder cancer T24 cell line

Ou, Liping; Guo, Yongcan; Luo, Chunli; Wu, Xiaohou; Zhao, Yi; Chen, Xiaozhong

doi:10.1016/j.cancergencyto.2010.01.021

Cited by 61 publications

(52 citation statements)

References 24 publications

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“…Also replicated independent studies have validated the association of PLCE1 polymorphism with esophageal cancer in Chinese Kazakh and Kashmiri population Malik et al, 2014). Recent studies have shown that, increased PLCE1 expression is related to the development of bladder cancer (Ou et al, 2010). PLCE1 overexpression also has positive effects on the transfer of the squamous cell carcinoma of the head and neck (Ma et al, 2011).…”

Section: Introductionmentioning

confidence: 83%

“…Recent studies have shown that PLCE1 may serve a critical function in the carcinogenesis process of esophageal and gastric cancers (Yu et al, 2014;Zhao et al, 2014) and have identified its involvement in various cancers, such as carcinoma of bladder (Ou et al, 2010), colorectal (Wang et al, 2012b), head and neck (Bourguignon et al, 2006), and skin (Bai et al, 2004) cancers. In previous studies of esophageal and gastric cancers, increased PLCE1 expression was significantly correlated with invasion depth, lymph node metastasis, and histologic grade.…”

Section: Discussionmentioning

confidence: 99%

“…Luo et al (2014) determined that PLCE1 is a suppressor of P53 in NSCLC, which was inconsistent with the results of Wang. Thereafter, a different pattern of PLCE1 expression is said to exist in different types of cancer, including cancers of the intestine, skin, colon, and rectum (Gonzalez-Garcia et al, 2005;Bourguignon, 2006;Wang et al, 2007;Baertschiger et al, 2009;Ou et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui

Peng²,

Su³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Section: Introductionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Cui

Peng²,

Su³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…Knockdown of PLCε with siRNA reduced the formation of the ruffling lamellipod in R-Ras cells. A corollary is that PLCE1 may lead to tumor formation by a chain of active reaction like regulating cell proliferation and differentiation, affecting the cell movement and cytoskeleton (Lad et al, 2006;Ou et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of PLCE1 Gene Silencing by RNA Interference on Cell Cycling and Apoptosis in Esophageal Carcinoma Cells

Zhao¹,

Wei²,

Yang³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Esophageal squamous cell carcinoma (ESCC) is one of the most malignancies with a poor prognosis. The phospholipase Cε gene (PLCE1) encodes a novel ras-related protein effector mediating the effects of R-Ras on the actin cytoskeleton and membrane protrusion. However, molecular mechanisms pertinent to ESCC are unclear. We therefore designed PLCE1-special small interfering RNA and transfected to esophageal squamous cell (EC) 9706 cells to investigat the effects of PLCE1 gene silencing on the cell cycle and apoptosis of ESCC and indicate its important role in the development of ESCC. Esophageal cancer tissue specimens and normal esophageal mucosa were obtained and assayed by immunohistochemical staining to confirm overexpression of PLCE1 in neoplasias. Fluorescence microscopy was used to examine transfection efficiency, while the result of PLCE1 silencing was examined by reverse transcription (RT-PCR). Flow cytometry and annexin V apoptosis assays were used to assess the cell cycle and apoptosis, respectively. Expression of cyclin D1 and caspase-3 was detected by Western-blotting. The level of PLCE1 protein in esophageal cancer tissue was significantly higher than that in normal tissue. After transfection, the expression of PLCE1 mRNA in EC 9706 was significantly reduced, compared with the control group. Furthermore, flow cytometry results suggested that the PLCE1 gene silencing arrested the cell cycle in the G0/G1 phase; apoptosis was significantly higher than in the negative control group and mock group. PLCE1 gene silencing by RNAi resulted in decreased expression of cyclin D1 and increased expression of caspase-3. Our study suggests that PLCE1 may be an oncogene and play an important role in esophageal carcinogenesis through regulating proteins which control cell cycling and apoptosis.

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“…However, the mechanism of action is not completely understood, and controversial data were reported. A number of evidences indicate that PI-PLC ε might favour cancer initiation and/or progression, as in bladder (Cheng et al 2011;Ou et al 2010), murine skin (Bai et al 2004, Oka 2010, Li et al 2009), head and neck cancers (Bourguignon et al 2006). The rs 2274223 polymorphism was significantly associated to increased risk of squamous cell carcinoma and gastric cancer (Hao 2013 By contrast, tumour suppressive role for PI-PLC ε was recently suggested in Ras-triggered cancers (Martins et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Ezrin silencing remodulates the expression of Phosphoinositide‐specific Phospholipase C enzymes in human osteosarcoma cell lines

Vasco

Leopizzi²,

Puggioni³

et al. 2014

Journal of Cell Communication and Signaling

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Ezrin, a protein belonging to the Ezrin, radixin and moesin (ERM) family, was engaged in the metastatic spread of osteosarcoma. The Protein 4.1, Ezrin, radixin, moesin (FERM) domain of Ezrin binds the membrane Phosphatydil inositol (4,5) bisphosphate (PIP2), a crucial molecule belonging to the Phosphoinositide (PI) signal transduction pathway. The cytoskeleton cross-linker function of Ezrin largely depends on membrane PIP2 levels, and thus upon the activity of related enzymes belonging to the PI-specific phospholipase C (PI-PLC) family. Based on the role of Ezrin in tumour progression and metastasis, we silenced the expression of Vil2 (OMIM *123900), the gene which codifies for Ezrin, in cultured human osteosarcoma 143B and Hs888 cell lines. After Ezrin silencing, the growth rate of both cell lines was significantly reduced and morphogical changes were observed. We also observed moderate variations both of selected PI-PLC enzymes within the cell and of expression of the corresponding PLC genes. In 143B cell line the transcription of PLCB1 decreased, of PLCG2 increased and of PLCE differed in a time-dependent manner. In Hs888, the expression of PLCB1 and of PLCD4 significantly increased, of PLCE moderately increased in a time dependent manner; the expression of PLCG2 was up-regulated. These observations indicate that Ezrin silencing affects the transcription of selected PLC genes, suggesting that Ezrin might influence the expression regulation of PI-PLC enzymes.

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RNA interference suppressing PLCE1 gene expression decreases invasive power of human bladder cancer T24 cell line

Cited by 61 publications

References 24 publications

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Prognostic Value of PLCE1 Expression in Upper Gastrointestinal Cancer: a Systematic Review and Meta-analysis

Effects of PLCE1 Gene Silencing by RNA Interference on Cell Cycling and Apoptosis in Esophageal Carcinoma Cells

Ezrin silencing remodulates the expression of Phosphoinositide‐specific Phospholipase C enzymes in human osteosarcoma cell lines

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