RNA interference supports a role for Nanchung–Inactive in mechanotransduction by the cockroach, Periplaneta americana, tactile spine

Hennenfent, Anneka; Liu, Hongxia; Torkkeli, Päivi H.; French, Andrew S.

doi:10.1007/s10158-019-0234-x

Cited by 7 publications

(6 citation statements)

References 45 publications

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“…Different combinations of the TRP subunits NompC, Nan and Iav contribute to mechanotransduction in several insect sensory organs [5,7,9,11,12,13]. We found orthologs to all these channels in the spider transcriptome, but in situ hybridization suggest that they are not expressed in the patellar mechanosensory cells and are unlikely to form their MET channels.…”

Section: Discussionmentioning

confidence: 77%

“…The deduced CsPiezo protein has 33 transmembrane domains and a molecular weight of 295 kDa. A previous phylogenetic analysis placed CsPiezo in a clade with other Chelicerata, separate from insects and mammalian clades [13]. In pairwise analysis the CsPiezo similarity to both mouse Piezo1 and 2 proteins is 37% and to the Drosophila Piezo protein it is 38%.…”

Section: Putative Mechanotransduction Channel Sequences In C Salei Hmentioning

confidence: 81%

“…InterPro scan and previous phylogenetic analysis [13] indicated that two C. salei TRP sequences are orthologous to the insect TRPN or NompC channels and were named CsNompC1 and 2. We determined that these sequences share 90% similarity to each other and 49% and 50% to the Drosophila NompC sequence.…”

Section: Putative Mechanotransduction Channel Sequences In C Salei Hmentioning

confidence: 99%

“…NompC also contributes to hearing in the Drosophila Johnston's organ [8], but a heteromeric TRPV channel Nan/Iav is likely responsible for transduction in these cells [9,10,11]. Nan/Iav may also form the MET channels in locust auditory neurons [12] and cockroach tactile spine neurons [13]. 3) Mutations in the human Transmembrane Channel-Like protein TMC1 lead to deafness, and deletion of mouse TMC1 removed MET currents in the inner ear hair cells [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Mechanotransduction Channel Piezo is Widely Expressed in the Spider, Cupiennius Salei, Mechanosensory Neurons and Central Nervous Systems

Johnson

Liu

Höger

et al. 2020

Preprint

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Mechanosensory neurons use mechanotransduction (MET) ion channels to detect mechanical forces and displacements. Proteins that function as MET channels have appeared multiple times during evolution and occur in at least four different families; the DEG/ENaC and TRP channels, and the TMC and Piezo proteins. We found twelve putative members of MET channel families in two spider transcriptomes, but detected only one, the Piezo protein, by in situ hybridization in their mechanosensory neurons. In contrast, probes for orthologs of TRP, ENaC or TMC genes that code MET channels in other species did not produce any signals in these cells. An antibody against C. salei Piezo detected the protein in all parts of their mechanosensory cells and in many neurons of the CNS. Unspecific blockers of MET channels, Ruthenium Red and GsMTx4, had no effect on the mechanically activated currents of the mechanosensory VS‑3 neurons, but the latter toxin reduced action potential firing when these cells were stimulated electrically. It is possible that the Piezo protein contributes to mechanosensory transduction in spider mechanosensilla, but it must have other functions in peripheral and central neurons.

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Section: Discussionmentioning

confidence: 77%

Section: Putative Mechanotransduction Channel Sequences In C Salei Hmentioning

confidence: 81%

Section: Putative Mechanotransduction Channel Sequences In C Salei Hmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mechanotransduction Channel Piezo is Widely Expressed in the Spider, Cupiennius Salei, Mechanosensory Neurons and Central Nervous Systems

Johnson

Liu

Höger

et al. 2020

Preprint

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“…NompC is also expressed and essential for the function of two other non-auditory mechanoreceptor types-bristle and campaniform-and when genetically knocked out results in a decrease of mechanotransduction (Kernan et al, 1994;Liang et al, 2010). However, transient knockdown of mechanotransduction channel candidates in cockroach, using RNAi, resulted in reduction of the bristle receptor response only for Nanchung and Inactive but not for NompC (Hennenfent et al, 2020). Thus, it appears that Nanchung-Inactive are, at least, drawing level with NompC as contenders for the auditory transduction channel in insects.…”

Section: Mode Of Transduction -Closing the Gap On The Identity Of The Transduction Ion Channel In Mammals And Insectsmentioning

confidence: 99%

Bridging the Gap Between Mammal and Insect Ears – A Comparative and Evolutionary View of Sound-Reception

Warren

Nowotny

2021

Front. Ecol. Evol.

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Insects must wonder why mammals have ears only in their head and why they evolved only one common principle of ear design—the cochlea. Ears independently evolved at least 19 times in different insect groups and therefore can be found in completely different body parts. The morphologies and functional characteristics of insect ears are as wildly diverse as the ecological niches they exploit. In both, insects and mammals, hearing organs are constrained by the same biophysical principles and their respective molecular processes for mechanotransduction are thought to share a common evolutionary origin. Due to this, comparative knowledge of hearing across animal phyla provides crucial insight into fundamental processes of auditory transduction, especially at the biomechanical and molecular level. This review will start by comparing hearing between insects and mammals in an evolutionary context. It will then discuss current findings about sound reception will help to bridge the gap between both research fields.

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Biochemical and subcellular characterization of a squid hnRNPA/B-like protein 2 in osmotic stress activated cells reflects molecular properties conserved in this protein family

Lopes

Lico

2022

Mol Biol Rep

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In previous works, we characterized a novel, strongly basic, squid hnRNPA/B-like Protein 2 in presynaptic terminals of squid neurons. Here, we show that squid hnRNPA/B-like Protein 2 are exclusively nuclear localization and relocated to cytoplasmic granules containing hnRNPA1 and Poly-A binding protein-1 (PABP-1) when the cells are treated with sorbitol. Also, we show an interaction of hnRNPA/B like Protein 2 with squid RNA, its interfered with dynamic of formation of hnRNPA/B like Protein 2 dimers, whereas possibly involved disulfide bounds and postranslations modification in a distinct stage of dimers formation. An understanding of the molecular and biochemical mechanisms involved in the stability of the dimeric form, and the regulation of the transition between monomeric and dimeric forms may bring insights into evolution of several neurodegenerative diseases.

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RNA interference supports a role for Nanchung–Inactive in mechanotransduction by the cockroach, Periplaneta americana, tactile spine

Cited by 7 publications

References 45 publications

Mechanotransduction Channel Piezo is Widely Expressed in the Spider, Cupiennius Salei, Mechanosensory Neurons and Central Nervous Systems

Mechanotransduction Channel Piezo is Widely Expressed in the Spider, Cupiennius Salei, Mechanosensory Neurons and Central Nervous Systems

Bridging the Gap Between Mammal and Insect Ears – A Comparative and Evolutionary View of Sound-Reception

Biochemical and subcellular characterization of a squid hnRNPA/B-like protein 2 in osmotic stress activated cells reflects molecular properties conserved in this protein family

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