RNA interference of the P450<i>CYP6CM1</i>gene has different efficacy in B and Q biotypes of<i>Bemisia tabaci</i>

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is one of the most important citrus pests. ACP is the vector of the phloem-limited bacteria Candidatus Liberibacter americanus and Candidatus Liberibacter asiaticus, the causal agents of the devastating citrus disease huanglongbing (HLB). The management of HLB is based on the use of healthy young plants, eradication of infected plants and chemical control of the vector. RNA interference (RNAi) has proven to be a promising tool to control pests and explore gene functions. Recently, studies have reported that target mRNA knockdown in many insects can be induced through feeding with double-stranded RNA (dsRNA). In the current study, we targeted the cathepsin D, chitin synthase and inhibitor of apoptosis genes of adult and nymph ACP by feeding artificial diets mixed with dsRNAs and Murraya paniculata leaves placed in dsRNAs solutions, respectively. Adult ACP mortality was positively correlated with the amount of dsRNA used. Both nymphs and adult ACP fed dsRNAs exhibited significantly increased mortality over time compared with that of the controls. Moreover, qRT-PCR analysis confirmed the dsRNA-mediated RNAi effects on target mRNAs. These results showed that RNAi can be a powerful tool for gene function studies in ACP and perhaps for HLB control.

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“…tabaci and H . armigera , revealing no obvious correlation of increase of dsRNA concentrations with the degree of target mRNA knockdown [25,47]. …”

Section: Discussionmentioning

confidence: 99%

Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri

et al. 2017

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“…For instance, through RNAi studies, several studies have shown that constitutive upregulation of CYPs mediates resistance to pyrethroid insecticides in the diamondback moth, Plutella xylostella 35 , the carmine spider mite, Tetranychus cinnabarinus 36 , and the red flour beetle, Tribolium castaneum 37 . Additionally, CYPs are involved in resistance to neonicotinoids in the small brown plant hopper, Laodelphax striatellus 38 and the tobacco whitefly, B. tabaci 39 , to organophosphates in the tobacco cutworm, Spodoptera litura 40 , and to abamectin in P . xylostella 41 .…”

Section: Discussionmentioning

confidence: 99%

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Kaplanoglu

Chapman

Scott

et al. 2017

Sci Rep

113

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Current control of insect pests relies on chemical insecticides, however, insecticide resistance development by pests is a growing concern in pest management. The main mechanisms for insecticide resistance typically involve elevated activity of detoxifying enzymes and xenobiotic transporters that break-down and excrete insecticide molecules. In this study, we investigated the molecular mechanisms of imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), an insect pest notorious for its capacity to develop insecticide resistance rapidly. We compared the transcriptome profiles of imidacloprid-resistant and sensitive beetle strains and identified 102 differentially expressed transcripts encoding detoxifying enzymes and xenobiotic transporters. Of these, 74 were up-regulated and 28 were down-regulated in the resistant strain. We then used RNA interference to knock down the transcript levels of seven up-regulated genes in the resistant beetles. Ingestion of double-stranded RNA successfully knocked down the expression of the genes for three cytochrome P450s (CYP6BQ15, CYP4Q3 and CYP4Q7), one ATP binding cassette (ABC) transporter (ABC-G), one esterase (EST1), and two UDP-glycosyltransferases (UGT1 and UGT2). Further, we demonstrated that silencing of CYP4Q3 and UGT2 significantly increased susceptibility of resistant beetles to imidacloprid, indicating that overexpression of these two genes contributes to imidacloprid resistance in this resistant strain.

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“…RNA concentrations were determined using a spectrophotometer73 and sent to the Beijing Genomics Institute (BGI, Shenzhen, China) when TREs expression were inhibited by the way of RNAi.…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference

Zhao

Yang

Shen

et al. 2016

Sci Rep

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RNA interference (RNAi) is an effective gene-silencing tool, and double stranded RNA (dsRNA) is considered a powerful strategy for gene function studies in insects. In the present study, we aimed to investigate the function of trehalase (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsRNA. The results showed that TRE1 and TRE2 had compensatory function, and the expression of each increased when the other was silenced. The total rate of insects with phenotypic deformities ranged from 19.83 to 24.36% after dsTRE injection, whereas the mortality rate ranged from 14.16 to 31.78%. The mRNA levels of genes involved in the chitin metabolism pathway in RNA-Seq and DGEP, namely hexokinase (HK), glucose-6-phosphate isomerase (G6PI) and chitinase (Cht), decreased significantly at 72 h after single dsTREs injection, whereas two transcripts of chitin synthase (CHS) genes decreased at 72 h after dsTRE1-1 and dsTREs injection. These results demonstrated that TRE silencing could affect the regulation of chitin biosynthesis and degradation, causing moulting deformities. Therefore, expression inhibitors of TREs might be effective tools for the control of planthoppers in rice.

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RNA interference of the P450CYP6CM1gene has different efficacy in B and Q biotypes ofBemisia tabaci

Abstract: RNA interference of the P450 CYP6CM1 gene reduced gene expression, increased mortality, and inhibited the ability to detoxify a pesticide or a plant secondary metabolite in both biotypes of B. tabaci, with better efficacy in B biotype than in Q biotype.

Cited by 47 publications

References 34 publications

Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri

Oral delivery of double-stranded RNAs induces mortality in nymphs and adults of the Asian citrus psyllid, Diaphorina citri

Overexpression of a cytochrome P450 and a UDP-glycosyltransferase is associated with imidacloprid resistance in the Colorado potato beetle, Leptinotarsa decemlineata

Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference

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