RNA Interference of Odorant-Binding Protein 2 (OBP2) of the Cotton Aphid, Aphis gossypii (Glover), Resulted in Altered Electrophysiological Responses

Rebijith, K. B.; Asokan, R.; Hande, H. Ranjitha; Kumar, N. K. Krishna; Krishna, V.; Vinutha, J.; Bakthavatsalam, N.

doi:10.1007/s12010-015-1869-7

Cited by 42 publications

(30 citation statements)

References 69 publications

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“…We then surmised that silencing OBPs might lead to behavioral changes in the phenotype. After all, gene silencing has already been successfully applied to investigate functions of OBPs in other insects (Chen et al, 2008; Pelletier et al, 2010; Rebijith et al, 2016). Of the two OBPs specific to male antennae, we selected RproOBP27 for these studies.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

et al. 2018

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Olfactory proteins mediate a wide range of essential behaviors for insect survival. Odorant binding proteins (OBPs) are small soluble olfactory proteins involved in the transport of odor molecules (=odorants) through the sensillum lymph to odorant receptors, which are housed on the dendritic membrane of olfactory sensory neurons also known as olfactory receptor neurons. Thus, a better understanding of the role(s) of OBPs from Rhodnius prolixus, one of the main vectors of Chagas disease, may ultimately lead to new strategies for vector management. Here we aimed at functionally characterize OBPs from R. prolixus. Genes of interest were selected using conventional bioinformatics approaches and subsequent quantification by qPCR. We screened and estimated expression in different tissues of 17 OBPs from R. prolixus adults. These analyses showed that 11 OBPs were expressed in all tissues, whereas six OBP genes were specific to antennae. Two OBP genes, RproOBP6 and RproOBP13, were expressed in both male and female antennae thus suggesting that they might be involved in the recognition of semiochemicals mediating behaviors common to both sexes, such host finding (for a blood meal). Transcripts for RproOBP17 and RproOBP21 were enriched in female antennae and possibly involved in the detection of oviposition attractants or other semiochemicals mediating female-specific behaviors. By contrast, RproOBP26 and RproOBP27 might be involved in the reception of sex pheromones given that their transcripts were highly expressed in male antennae. To test this hypothesis, we silenced RproOBP27 using RNAi and examined the sexual behavior of the phenotype. Indeed, adult males treated with dsOBP27 spent significantly less time close to females as compared to controls. Additionally, docking analysis suggested that RproOBP27 binds to putative sex pheromones. We therefore concluded that RproOBP27 might be a pheromone-binding protein.

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Section: Discussionmentioning

confidence: 99%

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

et al. 2018

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“…Several insects can nevertheless exhibit sustained RNAi for prolonged periods (Paim et al., ; Coleman et al., ; Khajuria et al., ), suggesting a system of signal amplification. Hemipterans appear to have a robust RNAi amplification system, as gene silencing has been demonstrated for 4 days (Rebijith et al., ), 6 days (Coleman et al., ), and 8 days (Tzin et al., ) after feeding with dsRNA. Coleman et al.…”

Section: Implementation Of Ernai In Pest Speciesmentioning

confidence: 99%

“…Practical considerations when designing eRNAi-based SIT strategies eRNAi is dose-dependent. The potency of gene silencing correlates with dsRNA concentration and period of exposure Tian et al, 2009Tian et al, , 2015Singh et al, 2013;Asokan et al, 2014;Yu et al, 2014;Li et al, 2015b;Ulrich et al, 2015;Whyard et al, 2015;Rebijith et al, 2016).…”

Section: Tablementioning

confidence: 99%

“…Several insects can nevertheless exhibit sustained RNAi for prolonged periods (Paim et al, 2013;Coleman et al, 2015;Khajuria et al, 2015), suggesting a system of signal amplification. Hemipterans appear to have a robust RNAi amplification system, as gene silencing has been demonstrated for 4 days (Rebijith et al, 2016), 6 days (Coleman et al, 2015), and 8 days (Tzin et al, 2015) after feeding with dsRNA. Coleman et al (2015) also report that nymphs born from RNA-treated mothers exhibited RNAi for 10 days post-feeding, suggesting that genetic variation between life-history stages might influence signal amplification.…”

Section: Tablementioning

confidence: 99%

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Implementing the sterile insect technique with RNA interference – a review

Darrington

Dalmay

Morrison

et al. 2017

Entomologia Exp Applicata

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We review RNA interference (RNAi) of insect pests and its potential for implementing sterile insect technique (SIT)‐related control. The molecular mechanisms that support RNAi in pest species are reviewed in detail, drawing on literature from a range of species including Drosophila melanogaster Meigen and Homo sapiens L. The underlying genes that enable RNAi are generally conserved across taxa, although variance exists in both their form and function. RNAi represents a plausible, non‐GM system for targeting populations of insects for control purposes, if RNAi effector molecules can be delivered environmentally (eRNAi). We consider studies of eRNAi from across several insect orders and review to what extent taxonomy, genetics, and differing methods of double‐stranded (ds) RNA synthesis and delivery can influence the efficiency of gene knockdown. Several factors, including the secondary structure of the target mRNA and the specific nucleotide sequence of dsRNA effector molecules, can affect the potency of eRNAi. However, taxonomic relationships between insects cannot be used to reliably forecast the efficiency of an eRNAi response. The mechanisms by which insects acquire dsRNA from their environment require further research, but the evidence to date suggests that endocytosis and transport channels both play key roles. Delivery of RNA molecules packaged in intermediary carriers such as bacteria or nanoparticles may facilitate their entry into and through the gut, and enable the evasion of host defence systems, such as toxic pH, that would otherwise attenuate the potential for RNAi.

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“…Similarly, Over-expression of Cytochrome P450 monooxygenase gene CYP6DA2 increased sensitivity to spirotetramat, alpha-cypermethrin and the toxicity of gossypol to cotton aphids [80,81]. Another study has demonstrated the silencing of the odorant-binding protein 2 ( OBP2 ) which resulted in impaired host-seeking and oviposition behavior of A. gossypii [82]. Furthermore, cotton transgenic plants expressing Amaranthus caudatus agglutinin (amaranthin) conferred enhanced resistance to A. gossypii [83].…”

Section: Rnai-based Approaches For the Control Of Insect Vectorsmentioning

confidence: 99%

RNA Interference in Insect Vectors for Plant Viruses

Kanakala

Ghanim

2016

Viruses

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Insects and other arthropods are the most important vectors of plant pathogens. The majority of plant pathogens are disseminated by arthropod vectors such as aphids, beetles, leafhoppers, planthoppers, thrips and whiteflies. Transmission of plant pathogens and the challenges in managing insect vectors due to insecticide resistance are factors that contribute to major food losses in agriculture. RNA interference (RNAi) was recently suggested as a promising strategy for controlling insect pests, including those that serve as important vectors for plant pathogens. The last decade has witnessed a dramatic increase in the functional analysis of insect genes, especially those whose silencing results in mortality or interference with pathogen transmission. The identification of such candidates poses a major challenge for increasing the role of RNAi in pest control. Another challenge is to understand the RNAi machinery in insect cells and whether components that were identified in other organisms are also present in insect. This review will focus on summarizing success cases in which RNAi was used for silencing genes in insect vector for plant pathogens, and will be particularly helpful for vector biologists.

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RNA Interference of Odorant-Binding Protein 2 (OBP2) of the Cotton Aphid, Aphis gossypii (Glover), Resulted in Altered Electrophysiological Responses

Cited by 42 publications

References 69 publications

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

Implementing the sterile insect technique with RNA interference – a review

RNA Interference in Insect Vectors for Plant Viruses

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