RNA interference of NADPH-cytochrome P450 reductase of the rice brown planthopper,<i>Nilaparvata lugens,</i>increases susceptibility to insecticides

Liu, Su; Liang, Qiao; Zhou, Wenwu; Jiang, Yuan; Zhu, Qi; Yu, Hang; Zhang, Chuan‐Xi; Gurr, Geoff M.; Zhu, Zhiliang

doi:10.1002/ps.3760

Cited by 45 publications

(57 citation statements)

References 48 publications

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“…Alternatively, once key genes endowing metabolic resistance are identified, specific double-stranded RNAs can be designed and delivered to plants to accurately silence or suppress the gene(s) conferring resistance. For example, silencing of P450 or other metabolic genes in insects by RNA interference (RNAi) has been shown to increase insect susceptibility to insecticides or inhibitory plant metabolites (Mao et al, 2007;Liu et al, 2014). RNAi can simultaneously target several genes; therefore, it is especially suitable for studying (and likely combating) metabolic herbicide resistance, as this is often under polygenic control .…”

Section: Can Metabolic Herbicide Cross-resistance Be Mitigated?mentioning

confidence: 99%

Metabolism-Based Herbicide Resistance and Cross-Resistance in Crop Weeds: A Threat to Herbicide Sustainability and Global Crop Production

2014

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Weedy plant species that have evolved resistance to herbicides due to enhanced metabolic capacity to detoxify herbicides (metabolic resistance) are a major issue. Metabolic herbicide resistance in weedy plant species first became evident in the 1980s in Australia (in Lolium rigidum) and the United Kingdom (in Alopecurus myosuroides) and is now increasingly recognized in several crop-weed species as a looming threat to herbicide sustainability and thus world crop production. Metabolic resistance often confers resistance to herbicides of different chemical groups and sites of action and can extend to new herbicide(s). Cytochrome P450 monooxygenase, glycosyl transferase, and glutathione S-transferase are often implicated in herbicide metabolic resistance. However, precise biochemical and molecular genetic elucidation of metabolic resistance had been stalled until recently. Complex cytochrome P450 superfamilies, high genetic diversity in metabolic resistant weedy plant species (especially cross-pollinated species), and the complexity of genetic control of metabolic resistance have all been barriers to advances in understanding metabolic herbicide resistance. However, next-generation sequencing technologies and transcriptome-wide gene expression profiling are now revealing the genes endowing metabolic herbicide resistance in plants. This Update presents an historical review to current understanding of metabolic herbicide resistance evolution in weedy plant species.

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Section: Can Metabolic Herbicide Cross-resistance Be Mitigated?mentioning

confidence: 99%

Metabolism-Based Herbicide Resistance and Cross-Resistance in Crop Weeds: A Threat to Herbicide Sustainability and Global Crop Production

2014

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“…The hemimetabolous brown planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) is one of the most destructive insect pests of rice that causes significant yield losses36. RNA interference (RNAi), which is a robust and powerful experimental tool, has been widely used to study gene functions through gene silencing34 in various piercing-sucking insects, including rice planthoppers3638394041. It has been reported that TRE regulates the expression of CHS in the cuticle and midgut of planthoppers, and the inhibition of chitin synthesis by suppressing or knocking down TRE leads to abnormal moulting and mortality.…”

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confidence: 99%

Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference

Zhao

Yang

Shen

et al. 2016

Sci Rep

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RNA interference (RNAi) is an effective gene-silencing tool, and double stranded RNA (dsRNA) is considered a powerful strategy for gene function studies in insects. In the present study, we aimed to investigate the function of trehalase (TRE) genes (TRE 1-1, TRE 1-2, and TRE-2) isolated from the brown planthopper Nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsRNA. The results showed that TRE1 and TRE2 had compensatory function, and the expression of each increased when the other was silenced. The total rate of insects with phenotypic deformities ranged from 19.83 to 24.36% after dsTRE injection, whereas the mortality rate ranged from 14.16 to 31.78%. The mRNA levels of genes involved in the chitin metabolism pathway in RNA-Seq and DGEP, namely hexokinase (HK), glucose-6-phosphate isomerase (G6PI) and chitinase (Cht), decreased significantly at 72 h after single dsTREs injection, whereas two transcripts of chitin synthase (CHS) genes decreased at 72 h after dsTRE1-1 and dsTREs injection. These results demonstrated that TRE silencing could affect the regulation of chitin biosynthesis and degradation, causing moulting deformities. Therefore, expression inhibitors of TREs might be effective tools for the control of planthoppers in rice.

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“…Originally, insecticide control was an important and very convenient way to control and decrease pest populations, but its improper use has led to pest resurgence and resistance, and to the accumulation of chemical residues (Xi et al 2014). In N. lugens , gene-function studies have also shown that RNAi of target genes could be used a pest-control strategy (Wang et al 2012, 2015; Xi et al 2014, 2015a,b; Liu et al 2015; Zhao et al 2016; Yang et al 2017). …”

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confidence: 99%

Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

Zhang¹,

Wang²,

Chen³

et al. 2017

Journal of Insect Science

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RNA interference has been used to study insects’ gene function and regulation. Glycogen synthase (GS) and glycogen phosphorylase (GP) are two key enzymes in carbohydrates’ conversion in insects. Glycogen content and GP and GS gene expression in several tissues and developmental stages of the Brown planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) were analyzed in the present study, using quantitative reverse-transcription polymerase chain reaction to determine their response to double-stranded trehalases (dsTREs), trehalose-6-phosphate synthases (dsTPSs), and validamycin injection. The highest expression of both genes was detected in the wing bud, followed by leg and head tissues, and different expression patterns were shown across the developmental stages analyzed. Glycogen content significantly decreased 48 and 72 h after dsTPSs injection and 48 h after dsTREs injection. GP expression increased 48 h after dsTREs and dsTPSs injection and significantly decreased 72 h after dsTPSs, dsTRE1-1, and dsTRE1-2 injection. GS expression significantly decreased 48 h after dsTPS2 and dsTRE2 injection and 72 h after dsTRE1-1 and dsTRE1-2 injection. GP and GS expression and glycogen content significantly decreased 48 h after validamycin injection. The GP activity significantly decreased 48 h after validamycin injection, while GS activities of dsTPS1 and dsTRE2 injection groups were significantly higher than that of double-stranded GFP (dsGFP) 48 h after injection, respectively. Thus, glycogen is synthesized, released, and degraded across several insect tissues according to the need to maintain stable trehalose levels.

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RNA interference of NADPH-cytochrome P450 reductase of the rice brown planthopper,Nilaparvata lugens,increases susceptibility to insecticides

Abstract: The results provide first evidence that NlCPR contributes to the susceptibility to beta-cypermethrin and imidacloprid in N. lugens.

Cited by 45 publications

References 48 publications

Metabolism-Based Herbicide Resistance and Cross-Resistance in Crop Weeds: A Threat to Herbicide Sustainability and Global Crop Production

Metabolism-Based Herbicide Resistance and Cross-Resistance in Crop Weeds: A Threat to Herbicide Sustainability and Global Crop Production

Functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using RNA interference

Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

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