RNA interference-mediated URG4 gene silencing diminishes cyclin D1 mRNA expression in HepG2 cells

Şatıroğlu-Tufan, N. Lale; Dodurga, Yavuz; D, Gok; Çetinkaya, Ayşegül; Ma, Fei

doi:10.4238/vol9-3gmr872

Cited by 22 publications

(10 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…URGCP/URG4 is associated with poor prognosis in hepatocellular carcinoma and its overexpression increased cellular entry into the G1/S transitional phase in hepatocellular carcinoma cells [30]. Furthermore, down-regulation of URG4 was shown to suppress cyclin D1 expression and cell proliferation in HepG2 hepatocellular carcinoma cells [31], further supporting our results.…”

Section: Discussionsupporting

confidence: 83%

Differential expression of store-operated calcium- and proliferation-related genes in hepatocellular carcinoma cells following TRPC1 ion channel silencing

et al. 2016

View full text Add to dashboard Cite

TRPC1 and store-operated Ca2+ (SOC) entry have previously been associated with hepatocellular carcinoma cell proliferation. The aim of the study was to determine genes and processes associated with TRPC1 down-regulation and the resulting increase of SOC entry and decrease in hepatocellular carcinoma cell proliferation. For this purpose, transcriptome analysis was performed to determine differentially expressed genes in TRPC1-silenced Huh7 cells. SOC entry- and proliferation-related genes correlated with TRPC1 down-regulation were also examined. Changes in SOC entry and cell proliferation were monitored in the TRPC1-silenced and parental cells and found to be significantly increased and decreased, respectively, in TRPC1-silenced cells. A total of 71 genes were significantly differentially expressed (40 up- and 31 down-regulated), including four mitogen-activated protein kinase (MAPK) signalling-associated genes. STIM1 levels were significantly up-regulated and negatively correlated with TRPC1 levels. In addition, expression of two cell cycle regulation genes, CDK11A/11B and URGCP, was observed to decrease, whereas ERBB3 and FGFR4, pro-survival genes, increased significantly in TRPC1-silenced cells. In conclusion, these results suggest reciprocal alterations in TRPC1 and STIM1 levels and a role for STIM1 in the regulation of SOC entry in TRPC1-silenced Huh7 cells. In addition to TRPC1, STIM1 may participate in Huh7 cell proliferation by regulating SOC entry. Alterations in MAPK signalling genes may be involved in diminished cell proliferation in TRPC1-silenced Huh7 cells. Similarly, changes in cell cycle regulating genes in TRPC1-silenced cells indicate possible cell cycle arrest along with compensatory up-regulation of ERBB3 growth factor receptor—amongst others—to maintain hepatocellular carcinoma cell proliferation.

show abstract

Section: Discussionsupporting

confidence: 83%

Differential expression of store-operated calcium- and proliferation-related genes in hepatocellular carcinoma cells following TRPC1 ion channel silencing

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Up-regulated gene 4 (URG4), located on 7 chromosome (7p13), was originally identified from hepatocellular carcinoma by a subtractive hybridization [4]. It may be involved in cell cycle progression through the regulation of cyclin D1 expression [4].…”

Section: Introductionmentioning

confidence: 99%

“…It may be involved in cell cycle progression through the regulation of cyclin D1 expression [4]. URG4 was strongly expressed in hepatitis B-infected liver and in hepatocellular carcinoma cells, where it contained with HBxAg, and was weakly expressed in uninfected liver, suggesting that URG4 was an effector of HBxAg in vivo [5].…”

Section: Introductionmentioning

confidence: 99%

URG4 upregulation is associated with tumor growth and poor survival in epithelial ovarian cancer

Zhou

2012

Arch Gynecol Obstet

View full text Add to dashboard Cite

show abstract

“…Over-expression of URG4/URGCP stimulated CCND1 mRNA expression, and RNAi-mediated URG4/URGCP silencing also diminished CCND1 mRNA expression in HepG2 cells. These results suggest that CCND1 up-regulation contributes importantly to the mechanism of URG4/URGCP -mediated hepatocellular growth [14, 16–18]. …”

Section: Introductionmentioning

confidence: 99%

Expressions of URG4/URGCP, cyclin D1, Bcl-2 , and Bax genes in retinoic acid treated SH-SY5Y human neuroblastoma cells

Dodurga

Gündoğdu

Koc

et al. 2013

View full text Add to dashboard Cite

Retinoic acid (RA) plays important roles in development, growth, and differentiation by regulating the expression of its target genes. The pro-apoptotic Bax gene may form channels through oligomerization in the mitochondrial membrane and facilitate the cytosolic release of cytochrome c. The anti-apoptotic Bcl-2 gene can inhibit this process. Up-regulated gene 4/Upregulator of cell proliferation (URG4/URGCP) is a novel gene located on 7p13. URG4/URGCP also stimulates cyclin D1 (CCND1) mRNA expression, and RNAi-mediated URG4/URGCP silencing diminishes CCND1 mRNA expression in HepG2 cells. In this study, the effects of RA treatment on URG4/URGCP, CCND1, Bcl-2 and Bax gene expression changes in undifferentiated and differentiated SHSY5Y neuroblastoma cells was analyzed. SHSY5Y cells were cultured in the appropriate conditions. To induce differentiation, the cells were treated with 10 micromolar RA in the dark for 3-10 days. SHSY5Y cells possess small processes in an undifferentiated state, and after treatment with RA, the cells developed long neurites, resembling a neuronal phenotype. Total RNA was isolated with Tri-Reagent. Expression profiles of the target genes were determined by semi-quantitative RT-PCR. According to the results, Bcl-2 and CCND1 gene expression levels were increased, while URG4/URGCP and Bax gene expression was decreased in RA treated cells compared to the control cells. Our preliminary results suggest that RA may induce cell proliferation and escape apoptosis using a novel pathway by the URG4/URGCP gene. Further investigations are needed to clarify more direct transcriptional targets of RA signaling and the interaction of RA pathways with other pro-regenerative signals.

show abstract

RNA interference-mediated URG4 gene silencing diminishes cyclin D1 mRNA expression in HepG2 cells

Cited by 22 publications

References 14 publications

Differential expression of store-operated calcium- and proliferation-related genes in hepatocellular carcinoma cells following TRPC1 ion channel silencing

Differential expression of store-operated calcium- and proliferation-related genes in hepatocellular carcinoma cells following TRPC1 ion channel silencing

URG4 upregulation is associated with tumor growth and poor survival in epithelial ovarian cancer

Expressions of URG4/URGCP, cyclin D1, Bcl-2 , and Bax genes in retinoic acid treated SH-SY5Y human neuroblastoma cells

Contact Info

Product

Resources

About