RNA Interference-Mediated Knockdown of Astrocyte Elevated Gene-1 Inhibits Growth, Induces Apoptosis, and Increases the Chemosensitivity to 5-Fluorouracil in Renal Cancer Caki-1 Cells

Wang, Peng; Yin, Bo; Shan, Liping; Zhang, Hui; Cui, Jun; Zhang, Mo; Song, Yi

doi:10.14348/molcells.2014.0081

Cited by 17 publications

(21 citation statements)

References 38 publications

(39 reference statements)

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“…Consistent with our results, previous studies showed that ectopic expression of AEG‐1 enhanced the proliferation and anchorage‐independent growth of cancer cells . Similar results have been found in breast cancer, cervical carcinoma, colon cancer, and renal cancer . In addition, AEG‐1 also increases the expression of multidrug resistance gene 1 and contributes to tumour chemoresistance …”

Section: Discussionsupporting

confidence: 81%

“…Ectopic expression of AEG-1 inhibits the apoptosis induced by serum starvation in primary human fetal astrocytes, 31 and AEG-1 silencing promotes apoptosis in renal cancer cells. 27 In the present study, apoptosis in melanoma cells was enhanced by AEG-1 silencing, which was further evidenced by increased chromatin condensation and increased cleaved caspase-3 levels. Both Bax and Bcl-2 proteins closely related to apoptosis.…”

Section: Discussionsupporting

confidence: 60%

“…The cell cycle is a determinant factor that affects the growth of cells. Previous studies have demonstrated that knockdown of AEG‐1 arrests the cell cycle at G 0 /G 1 . However, Nikpour et al.…”

Section: Discussioncontrasting

confidence: 40%

“…23,24 Similar results have been found in breast cancer, 24 cervical carcinoma, 25 colon cancer, 26 and renal cancer. 27 In addition, AEG-1 also increases the expression of multidrug resistance gene 1 and contributes to tumour chemoresistance. [27][28][29][30] The cell cycle is a determinant factor that affects the growth of Apoptosis is another important factor that determines cell growth.…”

Section: Discussionmentioning

confidence: 99%

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Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Zhang

Peng

Wang

et al. 2017

Clin Exp Pharma Physio

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Melanoma is an aggressive skin malignancy with a high mortality. Astrocyte elevated gene-1 (AEG-1), a downstream target of Ras and c-Myc, has been implicated in the development of multiple tumours, but its role in melanoma remains unclear. In the present study, the role of AEG-1 in melanoma was explored through AEG-1 silencing. Our results showed that silencing AEG-1 inhibited the proliferation of melanoma cells, induced cell cycle arrest, and reduced levels of cyclin A, cyclin B, cyclin D1, cyclin E, and cyclin-dependent kinase 2. AEG-1silencing also induced apoptosis in melanoma cells and altered the levels of cleaved caspase-3, B-cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein. Moreover, silencing AEG-1 suppressed the migration and invasion of melanoma cells, reduced the expressions and activities of matrix metallopeptidase (MMP)-2 and MMP-9, and inhibited the activation of the Wnt/β-catenin signalling pathway in melanoma cells. Furthermore, in vivo experiments revealed that AEG-1 silencing inhibited the growth of melanoma xenografts in nude mice. In summary, our study demonstrates an oncogenic role of AEG-1 in melanoma and suggests that AEG-1 may serve as a potential therapeutic target in the treatment of melanoma.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 60%

Section: Discussioncontrasting

confidence: 40%

Section: Discussionmentioning

confidence: 99%

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Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Zhang

Peng

Wang

et al. 2017

Clin Exp Pharma Physio

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“…20 The mRNA expression levels of OXGR1, AKT1, PIK3CG, PCNA, CDK1, CyclinD1 and FOXO3 were detected by quantitative real-time polymerase chain reaction (qPCR). 20 The mRNA expression levels of OXGR1, AKT1, PIK3CG, PCNA, CDK1, CyclinD1 and FOXO3 were detected by quantitative real-time polymerase chain reaction (qPCR).…”

Section: Quantitative Real-time Polymerase Chain Reaction Assaysmentioning

confidence: 99%

Yak OXGR1 promotes fibroblast proliferation via the PI3K/AKT pathways

Wang

Zhang

et al. 2018

J of Cellular Biochemistry

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Oxoglutarate receptor 1 (OXGR1), as one of the intermediates in G protein‐coupled receptors (GPCRs), plays a crucial role in the citric acid cycle receptor of α‐ketoglutarate and metabolism. GPCR can control the cell proliferation by regulating the downstream signaling of G protein signaling pathways. The PI3K/AKT pathway transmits the downstream signals of GPCRs and receptor tyrosine kinases. However, the speciﬁc role of OXGR1 promoting cell proliferation and differentiation are still unknown. In current study, the over‐expression vector and knockdown sequence of yak OXGR1 were transfected into yak fibroblasts, and the effects were detected by a series of assays. The results revealed that OXGR1 expression in yak lung parenchyma tissue was significantly higher than that of other tissues. In yak fibroblasts, the upregulated expression of OXGR1 resulted in activating the PIK3CG (downstream signal) of the PI3K/AKT1 pathway that can upregulated the expression of proliferation genes ( CDK1, PCNA, and CyclinD1) and promote cell proliferation. Conversely, the downregulated expression of OXGR1 inhibited cell proliferation via PI3K/AKT1 pathway. Cell cycle and cell proliferation assays demonstrated that over‐expression of OXGR1 can enhanced the DNA synthesis and promoted yak fibroblasts proliferation. While the conversely, knockdown of OXGR1 can decreased DNA synthesis and inhibited cell proliferation. These results illustrated that changes of OXGR1 expression can trigger the fibroblasts proliferation via PI3K/AKT signaling pathway, which indicating that OXGR1 is a novel regulator for cell proliferation and differentiation. Furthermore, these results provide evidence supporting the functional role of GPCRs‐PI3K‐AKT1 and OXGR1 in cell proliferation.

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Knockdown of astrocyte elevated gene-1 inhibited cell growth and induced apoptosis and suppressed invasion in ovarian cancer cells

Wang

Chen²,

Tong

2017

Gene

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RNA Interference-Mediated Knockdown of Astrocyte Elevated Gene-1 Inhibits Growth, Induces Apoptosis, and Increases the Chemosensitivity to 5-Fluorouracil in Renal Cancer Caki-1 Cells

Cited by 17 publications

References 38 publications

Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Silencing of astrocyte elevated gene‐1 inhibits proliferation and migration of melanoma cells and induces apoptosis

Yak OXGR1 promotes fibroblast proliferation via the PI3K/AKT pathways

Knockdown of astrocyte elevated gene-1 inhibited cell growth and induced apoptosis and suppressed invasion in ovarian cancer cells

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