RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice

Qiu, Lin; Fan, Jinxing; Zhang, Boyao; Wang, Xiaoping; Lei, Chaoliang; Lin, Yongjun; Ma, Weihua

doi:10.1016/j.jip.2017.03.001

Cited by 30 publications

(35 citation statements)

References 41 publications

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“…We previously determined that Cs APN1 and Cs APN2 are not involved in the toxicity of Cry2A to C . suppressalis (Qiu et al ., ) and the results presented here indicate that ALPs (ALP1, ALP2, ALP3, ALP4 and ALP6) are receptor proteins for Cry2Aa toxin. This may explain some of the variation in the susceptibility of C .…”

Section: Discussionmentioning

confidence: 97%

“…It has previously been suggested that APN1 and APN2 are Cry1Ca receptors in C . suppressalis (Qiu et al ., ). APN and ALP proteins could both play a role in the susceptibility of C .…”

Section: Discussionmentioning

confidence: 99%

“…suppressalis APN and CAD‐like proteins are involved in Cry toxicity in C . suppressalis (Yu et al ., ; Ma et al ., ; Qiu et al ., ; Wang et al ., ). The goal of this study was to determine if ALPs might also act as potential Cry1A, Cry2A and Cry1C receptors in the midgut of C .…”

Section: Discussionmentioning

confidence: 99%

“…Larvae from this colony were reared on an artificial diet following the protocol described in Qiu et al . ().…”

Section: Methodsmentioning

confidence: 97%

“…We have previously found evidence to suggest that APN1 and APN2 are involved in the toxicity of Bt transgenic rice to C . suppressalis (Qiu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

Qiu

Wang

et al. 2017

Insect Molecular Biology

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Insecticidal crystal (Cry) proteins produced by the bacterium Bacillus thuringiensis are highly toxic to lepidopteran pests. Strains of transgenic rice expressing cry genes have been developed that are resistant to rice pests. Understanding the mode of action of Cry toxins in rice pests will improve our ability to use them effectively as insecticides. In this study, we tested the hypothesis that alkaline phosphatases (ALPs) are involved in Cry1A, Cry2Aa and Cry1Ca toxicity in Chilo suppressalis, an important insect pest of rice crops in China. We first cloned three novel C. suppressalis alps (Csalps) from the larval midgut of C. suppressalis. RNA interference knockdown of six different Csalp genes (Csalp1, Csalp2, Csalp3, Csalp4, Csalp5 and Csalp6) showed that knockdown of three of these, Csalp1, Csalp2 and Csalp4, reduced larval mortality to the transgenic rice strain TT51, which expresses a fusion protein of Cry1Ab and Cry1Ac, whereas suppression of Csalp1, Csalp2, Csalp3, Csalp4 and Csalp6 transcripts decreased the susceptibility of larvae to the transgenic rice strain T2A-1, which expresses cry2Aa.Moreover, downregulation of Csalp1, Csalp2, Csalp3, Csalp4 and Csalp5 transcripts conferred significant tolerance to the transgenic rice strain T1C-19, which expresses cry1Ca. These results suggest that these ALPs play a key role in the toxicity of Cry1A, Cry2A and Cry1C to C. suppressalis.

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Section: Discussionmentioning

confidence: 97%

“…It has previously been suggested that APN1 and APN2 are Cry1Ca receptors in C . suppressalis (Qiu et al ., ). APN and ALP proteins could both play a role in the susceptibility of C .…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Larvae from this colony were reared on an artificial diet following the protocol described in Qiu et al . ().…”

Section: Methodsmentioning

confidence: 97%

“…We have previously found evidence to suggest that APN1 and APN2 are involved in the toxicity of Bt transgenic rice to C . suppressalis (Qiu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

Qiu

Wang

et al. 2017

Insect Molecular Biology

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Functional characterization of developmentally critical genes in the white‐backed planthopper: Efficacy of nanoparticle‐based dsRNA sprays for pest control

Guo

Liu

Long

et al. 2022

Pest Management Science

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BACKGROUND: Epidermal growth factor receptor (EGFR), zinc finger homeodomain-2 (zfh-2), Abdominal-A (Abd-A), and Abdominal-B (Abd-B) regulate the growth and development of the insect abdomen. However, their potential roles in pest control have not been fully assessed. The development of insecticide resistance to multiple chemistries in the white-backed planthopper (WBPH), a major pest of rice, has prompted interest in novel pest control approaches that are ecologically friendly. Although pest management approaches based on double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) have potential, their susceptibility to degradation limits large-scale field applications. These limitations, however, can be overcome with nanoparticle-dsRNA complexes that have greater environmental stability and improved cellular uptake.RESULTS: In this study, at 5 days post-injection, transcripts for the four gene targets were reduced relative to controls and all of the experimental groups exhibited significant phenotypic defects and increased mortality. To evaluate the potential of these gene targets for field applications, a nanocarrier-dsRNA spray delivery system was assessed for RNAi efficacy. At 11 days post-spray, significant phenotypic defects and increased mortality were observed in all experimental groups. CONCLUSION: Taken together, the results confirm the suitability of the target genes (SfEGFR, Sfzfh-2, SfAbd-A, and SfAbd-B) for pest management and demonstrate the efficacy of the nanocarrier spray system for inducing RNAi-mediated knockdown. As such, the study lays the foundation for the further development and optimization of this technology for large-scale field applications.

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New Technologies of Insect Control That Act Through the Gut

Terra,

Ferreira,

Silva

2023

Entomology in Focus

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RNA interference knockdown of aminopeptidase N genes decrease the susceptibility of Chilo suppressalis larvae to Cry1Ab/Cry1Ac and Cry1Ca-expressing transgenic rice

Cited by 30 publications

References 41 publications

Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

Downregulation of Chilo suppressalis alkaline phosphatase genes associated with resistance to three transgenic Bacillus thuringiensis rice lines

Functional characterization of developmentally critical genes in the white‐backed planthopper: Efficacy of nanoparticle‐based dsRNA sprays for pest control

New Technologies of Insect Control That Act Through the Gut

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