RNA interference for improving the outcome of islet transplantation

Li, Feng; Mahato, Ram I.

doi:10.1016/j.addr.2010.11.003

Cited by 31 publications

(29 citation statements)

References 260 publications

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“…The RNAi hallmark is the specificity of the dsRNA in which sequences are homologous to that of the target mRNA 2 . Experimentally, RNAi can be achieved by transfecting cells either directly using synthetic siRNA constructs or by using DNA vectors or viral vectors encoding short hairpin (sh) RNA 3 . Small interfering RNAs (siRNAs) comprise of specific sequences of 19-21 nucleotides that can be chemically synthesized consisting of sense and complementary anti-sense strands.…”

Section: Introductionmentioning

confidence: 99%

“…In comparison to the plasmid-based shRNA system, some viral-based shRNA types, such as the lentiviral vector, can integrate their shRNA-encoding genome stably into a host genome resulting in stable gene knockdown. The lentivirus is commonly used for www.scienceasia.org long term gene silencing because it can be transfected into a wide variety of cell lines and primary cells both in in vitro and in vivo 3,5,6 . The noncatalytic region of tyrosine kinase (Nck) is an adaptor protein expressed in a variety of tissue cells and cell lines.…”

Section: Introductionmentioning

confidence: 99%

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RNAi down-regulation of Nck1 adaptor protein in Jurkat T cells

Paensuwan¹,

Ngoenkam²,

Sanguansermsri³

et al. 2014

ScienceAsia

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RNA interference (RNAi) is a potent gene delivery system for studying the regulation of gene expression in a wide variety of eukaryotic cells. In the present study, different RNAi approaches, namely, synthetic small interfering RNA (siRNA) and plasmid-and lentivirus-based short hairpin RNA (shRNA) were investigated to assess the down-regulation of Nck1 protein efficiency in the Jurkat T cell line. Jurkat T cells treated with these three different systems substantially and specifically reduced the expression of the Nck1 protein but not that of the Nck2 protein. Although the three systems showed a similar Nck1 knockdown efficiency, they led to different T cell activation outcomes. After stimulation, CD69 expression and IL-2 production were impaired in Nck1-siRNA and plasmid-based Nck1-shRNA transfected Jurkat cells. However, these T cell activation outcomes were increased in lentiviral vector based Nck1-shRNA transfected cells. These data suggest that the outcomes from transfection with the shRNA based lentiviral vector differ from those of siRNA and shRNA-based plasmids although they provide the same gene silencing efficiency. The verification of suitable RNAi strategies to silence target genes is therefore necessary before using it in experiments.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

RNAi down-regulation of Nck1 adaptor protein in Jurkat T cells

Paensuwan¹,

Ngoenkam²,

Sanguansermsri³

et al. 2014

ScienceAsia

View full text Add to dashboard Cite

show abstract

“…Despite promising results in the development of miRNA therapeutics and successes on in vitro studies, limited progress has been made with in vivo studies or clinical trials. To increase the resistance to serum nuclease, avoid the activation of the innate immune system, and reduce off-target effects, chemical modifications of miRNA molecules [93] and different types of nano-vehicles have been proposed to treat different cancer types [94].…”

Section: Mirna Deliverymentioning

confidence: 99%

Drug Delivery Nanoparticles in Treating Chemoresistant Tumor Cells

Barrera

Daga

Ferrara

et al. 2018

CMC

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Intrinsic or acquired chemoresistance represents the main obstacle to the successful treatment of cancer patients. Several mechanisms are involved in multidrug resistance: decreased uptake of hydrophilic drugs, increase of energy dependent efflux, alteration of the redox state, alteration of apoptotic pathways, and modification of the tumor microenvironment. In recent years, several types of nanoparticles have been developed to overcome these obstacles and improve the accumulation and release of drugs at the pathological site. In this review we describe the main mechanisms involved in multidrug resistance and the nanovehicles which have been proposed to target specific aspects of this phenomenon.

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“…in fact, effective RNAi's against some specific genes are already commercially available and referred to as rnai therapy [1,13,21]. an outstanding advantage of the rnai method is the ability to regulate simultaneously and quantitatively more than one gene both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Development of a Protocol for Selection of GenesFit for the <i>In Vivo</i> Knockdown Method and its Application to Insulin Receptor Substrate Genesin Mice

et al. 2013

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Abstract:Prediabetes model mice in which more than one gene associated with diabetes is knocked down simultaneously are potentially useful for pharmaceutical and medical studies of diabetes. However, the effective conditions for sufficient knockdown in vivo are dependent on the intrinsic properties of the target genes. It is necessary to investigate which genes are applicable or not to the in vivo knockdown method. In this study, insulin receptor substrate 1 and 2 (Irs-1, Irs-2) were selected as target genes. Effective siRNAs against the respective genes were designed, and their efficacy was confirmed by cell-based experiments. Based on the results of siRNAs, shRNA expression vectors against Irs-1 and Irs-2 were constructed, respectively. Their efficacy was also confirmed by cell-based experiments. A hydrodynamic method was applied to the delivery of the vectors to mice. This method was found to be effective for predominant delivery to the liver by demonstrative delivery of an EGFP expression vector and successive histochemical analysis. Fifty micrograms of the shRNA expression vector was injected into the tail vein. After 24 h, the liver, pancreas, and muscle were isolated, and the expression levels of Irs-1 and Irs-2 were analyzed by quantitative RT-PCR. In the liver, Irs-2 was effectively knocked down to 60% of the control level, but Irs-1 was not influenced even under the same conditions. The protocol developed here is feasible for the selection of genes fit for in vivo knockdown method.

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RNA interference for improving the outcome of islet transplantation

Cited by 31 publications

References 260 publications

RNAi down-regulation of Nck1 adaptor protein in Jurkat T cells

RNAi down-regulation of Nck1 adaptor protein in Jurkat T cells

Drug Delivery Nanoparticles in Treating Chemoresistant Tumor Cells

Development of a Protocol for Selection of GenesFit for the <i>In Vivo</i> Knockdown Method and its Application to Insulin Receptor Substrate Genesin Mice

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