RNA Interference-Based Pesticides and Antiviral Agents: Microbial Overproduction Systems for Double-Stranded RNA for Applications in Agriculture and Aquaculture

Hashiro, Shuhei; Yasueda, Hisashi

doi:10.3390/app12062954

Cited by 17 publications

(12 citation statements)

References 116 publications

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“…This paper highlights the potential dsRNA target genes in aquatic pathogens that can reduce pathogen infection and improve the survival of aquatic host animals after silencing and also introduces species used as feed for oral-based dsRNA, such as E. coli and microalgae. An increasing number of studies has shown that the use of E. coli is a very inexpensive way to synthesize dsRNA, and E. coli is constantly being modified to obtain high yields ( Hashiro and Yasueda, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Potential dsRNAs can be delivered to aquatic for defense pathogens

Nie

Chen

Tang

et al. 2022

Front. Bioeng. Biotechnol.

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The use of antibiotics to facilitate resistance to pathogens in aquatic animals is a traditional method of pathogen control that is harmful to the environment and human health. RNAi is an emerging technology in which homologous small RNA molecules target specific genes for degradation, and it has already shown success in laboratory experiments. However, further research is needed before it can be applied in aquafarms. Many laboratories inject the dsRNA into aquatic animals for RNAi, which is obviously impractical and very time consuming in aquafarms. Therefore, to enable the use of RNAi on a large scale, the methods used to prepare dsRNA need to be continuously in order to be fast and efficient. At the same time, it is necessary to consider the issue of biological safety. This review summarizes the key harmful genes associated with aquatic pathogens (viruses, bacteria, and parasites) and provides potential targets for the preparation of dsRNA; it also lists some current examples where RNAi technology is used to control aquatic species, as well as how to deliver dsRNA to the target hydrobiont.

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Section: Discussionmentioning

confidence: 99%

Potential dsRNAs can be delivered to aquatic for defense pathogens

Nie

Chen

Tang

et al. 2022

Front. Bioeng. Biotechnol.

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“…Created with BioRender.com. et al 10.3389/ffunb.2022.977502 et al, 2019Islam et al, 2021;Hashiro and Yasueda, 2022). Indeed, the development of microbial-based dsRNA production systems has reduced the price of dsRNAs from ~US$ 12,000/g in 2008, to US$ 1/g, with Greenlight's GreenWorXTM system aiming to further lower the price of dsRNA synthesis to around US$ 0.5/g (reviewed in Guan et al, 2021).…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems

Ray¹,

Sahu²,

Aminedi³

et al. 2022

Front. Fungal Biol.

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Existing, emerging, and reemerging strains of phytopathogenic fungi pose a significant threat to agricultural productivity globally. This risk is further exacerbated by the lack of resistance source(s) in plants or a breakdown of resistance by pathogens through co-evolution. In recent years, attenuation of essential pathogen gene(s) via double-stranded (ds) RNA-mediated RNA interference (RNAi) in host plants, a phenomenon known as host-induced gene silencing, has gained significant attention as a way to combat pathogen attack. Yet, due to biosafety concerns regarding transgenics, country-specific GMO legislation has limited the practical application of desirable attributes in plants. The topical application of dsRNA/siRNA targeting essential fungal gene(s) through spray-induced gene silencing (SIGS) on host plants has opened up a transgene-free avenue for crop protection. However, several factors influence the outcome of RNAi, including but not limited to RNAi mechanism in plant/fungi, dsRNA/siRNA uptake efficiency, dsRNA/siRNA design parameters, dsRNA stability and delivery strategy, off-target effects, etc. This review emphasizes the significance of these factors and suggests appropriate measures to consider while designing in silico and in vitro experiments for successful RNAi in open-field conditions. We also highlight prospective nanoparticles as smart delivery vehicles for deploying RNAi molecules in plant systems for long-term crop protection and ecosystem compatibility. Lastly, we provide specific directions for future investigations that focus on blending nanotechnology and RNAi-based fungal control for practical applications.

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“…dsRNA can be produced using either in vitro transcription or in vivo expression in bacteria, yeast, microalgae and other species [130][131][132][133][134]. Bacteria are a low-cost alternative for the production of large amounts of dsRNA [135]. Escherichia coli HT115 (DE3) were first used as an heterologous dsRNA production system by [136].…”

Section: Dsrna Productionmentioning

confidence: 99%

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

Lucena-Leandro

Abreu²,

Vidal³

et al. 2022

IJMS

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Invasive insects cost the global economy around USD 70 billion per year. Moreover, increasing agricultural insect pests raise concerns about global food security constraining and infestation rising after climate changes. Current agricultural pest management largely relies on plant breeding—with or without transgenes—and chemical pesticides. Both approaches face serious technological obsolescence in the field due to plant resistance breakdown or development of insecticide resistance. The need for new modes of action (MoA) for managing crop health is growing each year, driven by market demands to reduce economic losses and by consumer demand for phytosanitary measures. The disabling of pest genes through sequence-specific expression silencing is a promising tool in the development of environmentally-friendly and safe biopesticides. The specificity conferred by long dsRNA-base solutions helps minimize effects on off-target genes in the insect pest genome and the target gene in non-target organisms (NTOs). In this review, we summarize the status of gene silencing by RNA interference (RNAi) for agricultural control. More specifically, we focus on the engineering, development and application of gene silencing to control Lepidoptera through non-transforming dsRNA technologies. Despite some delivery and stability drawbacks of topical applications, we reviewed works showing convincing proof-of-concept results that point to innovative solutions. Considerations about the regulation of the ongoing research on dsRNA-based pesticides to produce commercialized products for exogenous application are discussed. Academic and industry initiatives have revealed a worthy effort to control Lepidoptera pests with this new mode of action, which provides more sustainable and reliable technologies for field management. New data on the genomics of this taxon may contribute to a future customized target gene portfolio. As a case study, we illustrate how dsRNA and associated methodologies could be applied to control an important lepidopteran coffee pest.

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RNA Interference-Based Pesticides and Antiviral Agents: Microbial Overproduction Systems for Double-Stranded RNA for Applications in Agriculture and Aquaculture

Cited by 17 publications

References 116 publications

Potential dsRNAs can be delivered to aquatic for defense pathogens

Potential dsRNAs can be delivered to aquatic for defense pathogens

Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems

Current Scenario of Exogenously Induced RNAi for Lepidopteran Agricultural Pest Control: From dsRNA Design to Topical Application

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