RNA Interference-Based Forest Protection Products (FPPs) Against Wood-Boring Coleopterans: Hope or Hype?

Joga, Mallikarjuna Reddy; Mogilicherla, Kanakachari; Smagghe, Guy; Roy, Amit

doi:10.3389/fpls.2021.733608

Cited by 26 publications

(27 citation statements)

References 231 publications

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“…According to our knowledge, all the above-mentioned dsRNA delivery methods have not been tested in forest trees for pests and pathogens ever. Studies on dsRNA delivered through artificial diet (feeding), and injection are common among forest insect pests (Joga et al 2021 ). Moreover, none of the forest fungal pathogens have ever been considered, except for some pathogens from the genus Fusarium and Botrytis which are vital pathogens for crops (Koch et al 2016 ; Bennett et al 2020 ).…”

Section: Dsrna Delivery Methods In Woody Plantsmentioning

confidence: 99%

“…In the last few years, intensive studies have shown the efficiency of dsRNA against wood-boring forest pests like southern pine beetle, mountain pine beetles, emerald ash borer, Asian longhorn beetles, and Chinese white pine beetle (Joga et al 2021 ). The feeding of many candidate dsRNAs targeting, INHIBITOR OF APOPTOSIS ( IAP ), HEAT SHOCK 70-kDa PROTEIN COGNATE 3 ( HSP ), SHIBIRE ( SHI ), and injecting the β-FRUCTOFURANOSIDASE-ENCODING-2 ( AplaScrB-2 ) genes resulted in more than 80% of mortality to the emerald ash borer (Rodrigues et al 2017a , b , 2018 ).…”

Section: Dsrna Fungi- and Pesticide For Forest Insect Pests And Funga...mentioning

confidence: 99%

“…Control of many insect pests was reported using Nanoparticle encapsulation proving its efficiency. The nanoparticle-mediated dsRNA delivery could also be used with petiole absorption and trunk injection considered a GMO-free approach (Joga et al 2021 ). Moreover, the nanocarrier-mediated dsRNA transfer through spray has already displayed its success.…”

Section: Dsrna Pesticide Development For Forest Insect Pests and Fung...mentioning

confidence: 99%

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Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Singewar¹,

Fladung²

2023

Funct Integr Genomics

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Climate change alters the seasonal synchronization between plants and respective pests plus pathogens. The geographical infiltration helps to shift their hosts, resulting in novel outbreaks that damage forests and ecology. Traditional management schemes are unable to control such outbreaks, therefore unconventional and competitive governance is needed to manage forest pests and pathogens. RNA interference (RNAi) mediated double-stranded RNA (dsRNA) treatment method can be implemented to protect forest trees. Exogenous dsRNA triggers the RNAi-mediated gene silencing of a vital gene, and suspends protein production, resulting in the death of targeted pathogens and pests. The dsRNA treatment method is successful for many crop insects and fungi, however, studies of dsRNA against forest pests and pathogens are depleting. Pesticides and fungicides based on dsRNA could be used to combat pathogens that caused outbreaks in different parts of the world. Although the dsRNA has proved its potential, the crucial dilemma and risks including species-specific gene selection, and dsRNA delivery methods cannot be overlooked. Here, we summarized the major fungi pathogens and insect pests that have caused outbreaks, their genomic information, and studies on dsRNA fungi-and pesticides. Current challenges and opportunities in dsRNA target decision, delivery using nanoparticles, direct applications, and a new method using mycorrhiza for forest tree protection are discussed. The importance of affordable next-generation sequencing to minimize the impact on non-target species is discussed. We suggest that collaborative research among forest genomics and pathology institutes could develop necessary dsRNA strategies to protect forest tree species.

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Section: Dsrna Delivery Methods In Woody Plantsmentioning

confidence: 99%

Section: Dsrna Fungi- and Pesticide For Forest Insect Pests And Funga...mentioning

confidence: 99%

Section: Dsrna Pesticide Development For Forest Insect Pests and Fung...mentioning

confidence: 99%

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Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Singewar¹,

Fladung²

2023

Funct Integr Genomics

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“…Examples of targets potentially underlying insecticide resistance in L. decemlineata and explored using dsRNA-based approaches are shown in Table 4 . Furthermore, while it is challenging to highlight these studies in the current work, it is relevant to underline that such an approach has seems promising to control a variety of insect pests, including the fall armyworm Spodoptera frugiperda [ 78 ], the whitefly Bemisia tabaci [ 79 ], and the emerald ash borer Agrilus planipennis [ 80 ].…”

Section: Rnai and Insecticide Resistancementioning

confidence: 99%

Leveraging RNA Interference to Impact Insecticide Resistance in the Colorado Potato Beetle, Leptinotarsa decemlineata

Timani

Bastarache

Morin

2023

Insects

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The Colorado potato beetle, Leptinotarsa decemlineata Say, is a potato pest that can cause important economic losses to the potato industry worldwide. Diverse strategies have been deployed to target this insect such as biological control, crop rotation, and a variety of insecticides. Regarding the latter, this pest has demonstrated impressive abilities to develop resistance against the compounds used to regulate its spread. Substantial work has been conducted to better characterize the molecular signatures underlying this resistance, with the overarching objective of leveraging this information for the development of novel approaches, including RNAi-based techniques, to limit the damage associated with this insect. This review first describes the various strategies utilized to control L. decemlineata and highlights different examples of reported cases of resistances against insecticides for this insect. The molecular leads identified as potential players modulating insecticide resistance as well as the growing interest towards the use of RNAi aimed at these leads as part of novel means to control the impact of L. decemlineata are described subsequently. Finally, select advantages and limitations of RNAi are addressed to better assess the potential of this technology in the broader context of insecticide resistance for pest management.

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“…As a pathogen-specific and environmentally friendly technology, SIGS offers excellent potential for plant protection since it does not require transgenic plants, in contrast to Host-Induced Gene Silencing (HIGS) (Wang and Jin, 2017). For SIGS technology to be successful, several essential requirements must be met, including correct selection of target genes, proper design of the dsRNAs (Joga et al, 2021), as well as ensuring that the pathogens can internalize dsRNA molecules (Qiao et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest disease

Bocos-Asenjo,

Amin,

Mosquera

et al. 2024

Preprint

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Global change is exacerbating the prevalence of plant diseases caused by pathogenic fungi in forests worldwide. The conventional use of chemical fungicides, which is commonplace in agricultural settings, is not sanctioned for application in forest ecosystems, so novel control strategies are imperative. The promising approach SIGS (Spray-Induced Gene Silencing) involves the external application of specific double-stranded RNA (dsRNA), which can modulate the expression of target genes through environmental RNA interference in eukaryotes. SIGS exhibited notable success in reducing virulence when deployed against some crop fungal pathogens, such as Fusarium graminearum, Botrytis cinerea and Sclerotinia sclerotiorum, among others. However, there is a conspicuous dearth of studies evaluating the applicability of SIGS for managing forest pathogens. This research aimed to determine whether SIGS could be used to control Fusarium circinatum, a widely impactful forest pathogen that causes Pine Pitch Canker disease. To achieve this, we designed and produced though a bacterial synthesis, dsRNA molecules to target fungal essential genes involved to vesicle trafficking (Vps51, DCTN1, and SAC1), signal transduction (Pp2a, Sit4, Ppg1, and Tap42), and cell wall biogenesis (Chs1, Chs2, Chs3b, Gls1) metabolic pathways. We confirmed that F. circinatum is able to uptake externally applied dsRNA, triggering an inhibition of the pathogens virulence. Furthermore, this study pioneers the demonstration that recurrent applications of dsRNAs in SIGS are more effective in protecting plants than single applications. Therefore, SIGS emerges as an effective and sustainable approach for managing plant pathogens, showcasing its efficacy in controlling a globally significant forest pathogen subject to quarantine measures.

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RNA Interference-Based Forest Protection Products (FPPs) Against Wood-Boring Coleopterans: Hope or Hype?

Abstract: Graphical AbstractThree-tier impact of forest protection products (FPPs).

Cited by 26 publications

References 231 publications

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities

Leveraging RNA Interference to Impact Insecticide Resistance in the Colorado Potato Beetle, Leptinotarsa decemlineata

Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest disease

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