Recently several investigators have shown the existence of a sequential pathway of genic expression from DNA to specific protein synthesis through RNA in mammalian cells (Schweet et al. 1958; Weiss 1960;Hiatt 1962; Sibatani et al. 1962). On the other hand it is conceivable that there are many complicated regulatory mechanisms controlling genic expression in higher animals. In order to reveal these mechanisms of regulation one useful approach is to isolate molecular genetic information at different steps in the chemical sequences of genic expression. Several such attemps have been successful. For instance, in a cell free system prepared from rabbit reticulocytes, a messenger RNA-like fraction obtained from sheep reticulocytes could produce sheep hemoglobin (Lamfrom 1962). In intact mammalian cells, other heterologous proteins could be synthesized by the addition of exogenous RNA (Niu et al. 1961; Weisberger 1962) or DNA (Szybalska and Szybalski 1963).The present study represents an attempt to obtain molecular genetic information (RNA) related to myeloma protein synthesis. Specifically, the attempt was made to induce synthesis of gamma myeloma protein by Ehrlich ascites tumor cells by exposing these cells to a high concentration of RNA extracted from a plasma cell tumor. The Ehrlich ascites cells are incapable of synthesizing this protein without this treatment. Moreover, dependence of gamma myeloma protein synthesis on DNA was tested by actinomycin D, which inhibits DNA transcription.
MATERIALS AND METHODSTumor strains: The plasma cell tumor (X5563) used in this study was kindly supplied by Dr. M. Potter of the National Institutes of Health, U.S.A., in 1962. Since then it has been maintained in C3H mice by serial subcutaneous transplantation every two weeks. The stable character of the tumor cells to produce a gamma myeloma protein in large amount was ascertained by means of paper electrophoresis.As a recipient of the plasma cell tumor RNA, hyperdiploid Ehrlich ascites tumor cells (ELD) were employed.