RNA editing implicated in chloroplast-to-nucleus communication

Larkin, Robert M.

doi:10.1073/pnas.1905566116

Cited by 7 publications

(2 citation statements)

References 11 publications

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“…In seed plants, RNA editing in the chloroplast converts cytidine to uridine (C to U RNA editing), some of which can affect protein function 54,55 . RNA editing in chloroplast was reported to contribute to chloroplast-to-nucleus signaling 56,57 . We predicted RNA editing sites in the V. pubescens and C. papaya chloroplast genomes.…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis of chloroplast genomes in Vasconcellea pubescens A.DC. and Carica papaya L.

Lin

Zhou

et al. 2020

Sci Rep

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The chloroplast genome is an integral part of plant genomes in a species along with nuclear and mitochondrial genomes, contributing to adaptation, diversification, and evolution of plant lineages. In the family Caricaceae, only the Carica papaya chloroplast genome and its nuclear and mitochondrial genomes were sequenced, and no chloroplast genome-wide comparison across genera was conducted. Here, we sequenced and assembled the chloroplast genome of Vasconcellea pubescens A.DC. using Oxford Nanopore Technology. The size of the genome is 158,712 bp, smaller than 160,100 bp of the C. papaya chloroplast genome. And two structural haplotypes, LSC_IRa_SSCrc_IRb and LSC_IRa_SSC_IRb, were identified in both V. pubescens and C. papaya chloroplast genomes. The insertion-deletion mutations may play an important role in Ycf1 gene evolution in family Caricaceae. Ycf2 is the only one gene positively selected in the V. pubescens chloroplast genome. In the C. papaya chloroplast genome, there are 46 RNA editing loci with an average RNA editing efficiency of 63%. These findings will improve our understanding of the genomes of these two crops in the family Caricaceae and will contribute to crop improvement.

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Section: Resultsmentioning

confidence: 99%

Comparative analysis of chloroplast genomes in Vasconcellea pubescens A.DC. and Carica papaya L.

Lin

Zhou

et al. 2020

Sci Rep

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“…Moreover, compared to wild type (WT), differential RNA-editing profiles were observed in both gun1 null mutant and the MORF2 overexpression lines when treated with the bleaching herbicide norflurazon (NF) ( Zhao et al., 2019 ). Although this discovery suggests a possible interplay between RNA editing and RS, it remains elusive whether and how the observed RNA-editing changes result from RS or vice versa ( Larkin, 2019 ; Zhao et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing

et al. 2023

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Retrograde signaling modulates the expression of nuclear genome-encoded organelle proteins to adjust organelle function in response to environmental cues. MULTIPLE ORGANELLAR RNA EDITING FACTOR 2 (MORF2) was initially recognized as a plastidial RNA-editing factor but recently shown to interact with GUN1. Given the central role of GUN1 in chloroplast retrograde signaling and the unviable phenotype of morf2 mutants that is inconsistent with many viable mutants involved in RNA editing, we hypothesized that MORF2 has functions either dosage dependent or beyond RNA editing. Using an inducible Clustered Interspaced Short Palindromic Repeat interference (iCRISPRi) approach, we were able to reduce the MORF2 transcripts in a controlled manner. In addition to MORF2-dosage dependent RNA-editing errors, we discovered that reducing MORF2 by iCRISPRi stimulated the expression of stress responsive genes, triggered plastidial retrograde signaling, repressed ethylene signaling and skotomorphogenesis, and increased accumulation of hydrogen peroxide. These findings along with previous discoveries suggest that MORF2 is an effective regulator involved in plastidial metabolic pathways whose reduction can readily activate multiple retrograde signaling molecules possibly involving reactive oxygen species to adjust plant growth. In addition, our newly developed iCRISPRi approach provided a novel genetic tool for quantitative reverse genetics studies on hub genes in plants.

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Prediction of RNA editing sites in maize under salt stress through transcriptomic approaches

Alshaya,

Uzair,

Rehman

et al. 2024

Plant Biotechnol Rep

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RNA editing implicated in chloroplast-to-nucleus communication

Cited by 7 publications

References 11 publications

Comparative analysis of chloroplast genomes in Vasconcellea pubescens A.DC. and Carica papaya L.

Comparative analysis of chloroplast genomes in Vasconcellea pubescens A.DC. and Carica papaya L.

MORF2-mediated plastidial retrograde signaling is involved in stress response and skotomorphogenesis beyond RNA editing

Prediction of RNA editing sites in maize under salt stress through transcriptomic approaches

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