RNA-Dependent RNA Targeting by CRISPR-Cas Systems: Characterizations and Applications

Gunitseva, Natalia; Evteeva, Marta; Borisova, Anna S.; Патрушев, М. В.; Subach, Fedor V.

doi:10.3390/ijms24086894

Cited by 9 publications

(3 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is possible to improve seed size or overall grain output in wheat by altering genes involved in hormone cascades, seed development, or stress responses [43]. Furthermore, with the RNA-targeting potential of Cas13, post-transcriptional modifications can be realized, offering another layer of gene regulation that might influence seed development and grain yield [44].…”

Section: Crispr Cas9; Cas13mentioning

confidence: 99%

Genetic and Genomic Pathways to Improved Wheat (Triticum aestivum L.) Yields: A Review

Chachar,

Fan,

Chachar

et al. 2024

Agronomy

View full text Add to dashboard Cite

Wheat (Triticum aestivum L.) is a fundamental crop essential for both human and animal consumption. Addressing the challenge of enhancing wheat yield involves sophisticated applications of molecular genetics and genomic techniques. This review synthesizes current research identifying and characterizing pivotal genes that impact traits such as grain size, number, and weight, critical factors influencing overall yield. Key genes including TaSPL17, ABP7, TaGNI, TaCKX6, TaGS5, TaDA1, WAPO1, TaRht1, TaTGW-7A, TaGW2, TaGS5-3A, TaSus2-2A, TaSus2-2B, TaSus1-7A, and TaSus1-7B are examined for their roles in these traits. The review also explores genes responsive to environmental changes, which are increasingly significant under current climate variability. Multi-trait regulatory genes and quantitative trait loci (QTLs) that contribute to these traits are discussed, highlighting their dual influences on grain size and yield. Furthermore, the paper underscores the utility of emerging technologies such as CRISPR/Cas9, Case13, and multi-omics approaches. These innovations are instrumental for future discoveries and are poised to revolutionize wheat breeding by enabling precise genetic enhancements. Facing unprecedented challenges from climate change, the identification and utilization of these candidates is crucial. This review aims to be a comprehensive resource for researchers, providing an integrative understanding of complex traits in wheat and proposing new avenues for research and crop improvement strategies.

show abstract

Section: Crispr Cas9; Cas13mentioning

confidence: 99%

Genetic and Genomic Pathways to Improved Wheat (Triticum aestivum L.) Yields: A Review

Chachar,

Fan,

Chachar

et al. 2024

Agronomy

View full text Add to dashboard Cite

show abstract

“…Various strategies have been pursued for targeting RNA, which we categorize into two groups ( Figures 1A, B ): 1) strategies that directly target RNA, including antisense oligonucleotides (ASO) ( Crooke et al, 2018 ) and CRISPR gene editing ( Gunitseva et al, 2023 ), and 2) small molecules designed to specifically recognize RNAs of interest. ASOs have yielded promising results in human trials, paving the way for the use of RNA as therapeutic targets ( Oren et al, 2022 ; Tran et al, 2022 ; Dindot et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Using the structural diversity of RNA: protein interfaces to selectively target RNA with small molecules in cells: methods and perspectives

Li,

Bouhss,

Clément

et al. 2023

Front. Mol. Biosci.

View full text Add to dashboard Cite

In recent years, RNA has gained traction both as a therapeutic molecule and as a therapeutic target in several human pathologies. In this review, we consider the approach of targeting RNA using small molecules for both research and therapeutic purposes. Given the primary challenge presented by the low structural diversity of RNA, we discuss the potential for targeting RNA: protein interactions to enhance the structural and sequence specificity of drug candidates. We review available tools and inherent challenges in this approach, ranging from adapted bioinformatics tools to in vitro and cellular high-throughput screening and functional analysis. We further consider two critical steps in targeting RNA/protein interactions: first, the integration of in silico and structural analyses to improve the efficacy of molecules by identifying scaffolds with high affinity, and second, increasing the likelihood of identifying on-target compounds in cells through a combination of high-throughput approaches and functional assays. We anticipate that the development of a new class of molecules targeting RNA: protein interactions to prevent physio-pathological mechanisms could significantly expand the arsenal of effective therapeutic compounds.

show abstract

“…2023, 24, 17105 2 of 14 interest to scientists because they contain one effector, which allows them to be conveniently used as tools for genomic engineering and nucleic acid detection [15][16][17][18]. Class 2 CRISPR-Cas systems include types II, V, and VI, with corresponding effectors Cas9, Cas12, and Cas13 [19,20]. Type V CRISPR-Cas systems vary by a single Cas12 effector that contains a conserved RuvC-like endonuclease domain regulated by RNA [21].…”

Section: Introductionmentioning

confidence: 99%

A New RNA-Dependent Cas12g Nuclease

Gunitseva,

Evteeva,

Korzhenkov

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

The development of RNA-targeting CRISPR-Cas systems represents a major step forward in the field of gene editing and regulation. RNA editing presents a viable alternative to genome editing in certain scenarios as it offers a reversible and manageable approach, reducing the likelihood of runaway mutant variants. One of the most promising applications is in the treatment of genetic disorders caused by mutations in RNA molecules. In this study, we investigate a previously undescribed Cas12g nuclease which was found in metagenomes from promising thermophilic microbial communities during the expedition to the Republic of North Ossetia—Alania in 2020. The method outlined in this study can be applied to other Cas orthologs and variants, leading to a better understanding of the CRISPR-Cas system and its enzymatic activities. The cis-cleavage activity of the new type V-G Cas effector was indicated by in vitro RNA cleavage experiments. While CRISPR-Cas systems are known for their high specificity, there is still a risk of unintended cleavage of nontargeted RNA molecules. Ultimately, the search for new genome editing tools and the study of their properties will remove barriers to research in this area. With continued research and development, we may be able to unlock their full potential.

show abstract

RNA-Dependent RNA Targeting by CRISPR-Cas Systems: Characterizations and Applications

Cited by 9 publications

References 89 publications

Genetic and Genomic Pathways to Improved Wheat (Triticum aestivum L.) Yields: A Review

Genetic and Genomic Pathways to Improved Wheat (Triticum aestivum L.) Yields: A Review

Using the structural diversity of RNA: protein interfaces to selectively target RNA with small molecules in cells: methods and perspectives

A New RNA-Dependent Cas12g Nuclease

Contact Info

Product

Resources

About