RNA‐dependent DNA polymerase associated with a simian lymphoid cell line derived from a Herpesvirus saimiri‐induced lymphoma

Abstract

This communication describes the spontaneous appearance of a membrane-bound RNA-dependent D N A polymerase in a simian lymphoma cell line that was established from a lymphoma tumor mass previously induced by Herpesvirus saimiri in an owl monkey. This membrane-bound R NA-dependent DNA polymerase fraction possesses many biochemical properties analogous to those of known primate and murine R N A tumor viruses. Characteristics of this RNA-dependent D N A polymerase are clearly distinct from those of the D N A polymerase of Herpesvirus saimiri. This particular fraction incorporates tritiated ribonucleosides into R N A in an in vitro system, and it sediments at 1.15-1.16 glcm 3. It is not induced to greater production by halogenated deoxyuridines. The current observations were made at a time when the host cells were no longer producing Herpesvirus saimiri.

Influence of interferon on the synthesis of virus particles in oncornavirus carrier cell lines. III. Survey of effects on A‐, B‐ and C‐type oncornaviruses

“…sponded to a smaller number of A-type particles detectable in cell sections by electron microscopy. Characterization of extraction procedure for To eliminate the accessory material the virus A-type particles pellet was prepared on a e = 1.18 sucrose The extraction procedure, described in detail in the section " Material and Methods ", involved: (1) thorough washing of cells; (2) homogenization in a high-potassium, EDTA-containing buffer to ensure dissociation of polysomes (Yang et al, 1974); (3) treatment with ribonuclease to reduce all non-envelopped RNA to non-precipitable oligonucleotides; (6) ultracentrifugation and resuspension of the virus pellet; (5) equilibrium centrifugation on a sucrose gradient 5-60 % and fractionation.…”

Evidence for type‐C retrovirus production by Burkitt's lymphoma‐derived cell line

“…Several lines of investigation on animal systems seem to point to a possible interaction between a DNA herpesvirus and an RNA tumor virus as responsible for oncogenesis. Thus, it was demonstrated that UV-irradiated herpes simplex virus types 1 and 2 activated the production of an endogenous type-C virus of mouse (Hampar et al, 1976;Reed and Rapp, 1976;Hampar et al, 1977a, b ) , and that oncornavirus-like particles were present in a simian lymphoid cell line derived from a lymphoma induced by herpesvirus saimiri (Yang et al, 1974); furthermore, the presence of both an oncornavirus and a herpesvirus was necessary for the manifestation of neoplastic disease in guinea-pigs (Hsiung et al, 1973), while the association of the Marek's disease herpesvirus with an avian leukosis type-C virus caused enhanced tumor responses and mortality (Peters et al, 1973;Frankel et al, 1974;Smith et al, 1975;Campbell et al, 1978).…”

Induction of murine p30 by superinfecting herpesviruses

“…In addition, studies with a guinea pig herpesvirus and oncornavirus suggest that both viruses may be involved in hyperplasia of spleen and lymph nodes and leukemia in guinea pigs (6). RNAdependent DNA polymerase activity has also been associated with a simian lymphoid cell line derived from Herpesvirus saimiri-induced lymphoma (14). Since the possibility exists that both viruses may be necessary for the induction of neoplastic disease, an investigation of the interaction of oncogenic herpesviruses and resident type C viruses should lead to better understanding of the viral etiology of these diseases.…”