RNA-dependent disassembly of nuclear bodies

Musinova, Yana R.; Lisitsyna, Olga M.; Sorokin, Dmitry V.; Arifulin, Eugene A.; Смирнова, Т. А.; Zinovkin, Roman A.; Potashnikova, Daria; Vassetzky, Yegor S.; Sheval, Eugene V.

doi:10.1242/jcs.189142

Cited by 7 publications

(4 citation statements)

References 57 publications

(66 reference statements)

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“…The formation of interphase prenucleolar bodies (iPNBs) associated with unprocessed rRNA is induced in cells under hypotonic stress ( 74 ). Here, we show that the inhibition of RNA Pol I by Act D treatment induces the aggregation of nucleolin speckles in the nucleoplasm, which is further enhanced upon lamin B2 depletion ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function

Gupta

Sengupta

2017

Molecular and Cellular Biology

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The nucleolus is required for ribosome biogenesis. Human cells have 2 or 3 nucleoli associated with nucleolar organizer region (NOR)-bearing chromosomes. An increase in number and altered nucleolar morphology define cancer cells. However, the mechanisms that modulate nucleolar morphology and function are unclear. Here we show that in addition to localizing at the nuclear envelope, lamin B2 localizes proximal to nucleolin at the granular component (GC) of the nucleolus and associates with the nucleolar proteins nucleolin and nucleophosmin. Lamin B2 knockdown severely disrupted the nucleolar morphology, which was rescued to intact and discrete nucleoli upon lamin B2 overexpression. Furthermore, two mutually exclusive lamin B2 deletion mutants, ΔHead and ΔSLS, rescued nuclear and nucleolar morphology defects, respectively, induced upon lamin B2 depletion, suggesting independent roles for lamin B2 at the nucleolus and nuclear envelope. Lamin B2 depletion increased nucleolin aggregation in the nucleoplasm, implicating lamin B2 in stabilizing nucleolin within the nucleolus. Lamin B2 knockdown upregulated nucleolus-specific 45S rRNA and upstream intergenic sequence (IGS) transcripts. The IGS transcripts colocalized with aggregates of nucleolin speckles, which were sustained in the nucleoplasm upon lamin B2 depletion. Taken together, these studies uncover a novel role for lamin B2 in modulating the morphology, dynamics, and function of the nucleolus.

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Section: Discussionmentioning

confidence: 99%

Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function

Gupta

Sengupta

2017

Molecular and Cellular Biology

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“…The optimal time for the start of iPNB imaging is 25-35 min after the return to isotonic conditions. At this stage, the number of iPNBs is substantially reduced [7], facilitating the imaging and analysis of iPNBs tracks. Cells can be incubated directly on the microscope stage, if the thermostatic chamber allows to control both CO 2 and temperature.…”

Section: Ipnbs Formation May Start During Hypotonic Treatment If 20% ...mentioning

confidence: 99%

“…iPNBs are formed in interphase nuclei from partially disassembled nucleoli when cells return to isotonic conditions from a hypotonic shock [4]. iPNBs contain nucleolar proteins NPM1 (B23 or nucleophosmin) [4][5][6][7], NCL (C23 or nucleolin) [8] and pre-rRNAs [7]. In contrast to the majority of other NBs, a large number (several hundred per nucleus) of iPNBs is induced simultaneously, and they are uniformly distributed within the nuclear space [7].…”

Section: Introductionmentioning

confidence: 99%

Live-Cell Imaging and Analysis of Nuclear Body Mobility

et al. 2020

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The cell nucleus contains different domains and nuclear bodies; their position relative to each other inside the nucleus can vary depending on the physiological state of the cell. Changes in the three-dimensional organization are associated with the mobility of individual components of the nucleus. In this paper, we present a protocol for live-cell imaging and analysis of nuclear body mobility. Unlike many similar protocols, our image analysis pipeline includes non-rigid compensation for global nucleus motion before particle tracking and trajectory analysis, leading to precise detection of intranuclear movements. The protocol described can be easily adapted to work with most cell lines and nuclear bodies.

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“…On the other hand, maintenance of high RNA concentration prevents the formation of insoluble RNP complexes [6]. Studies of the nucleolus reassembly after stress suggest that RNA species, which initiates nuclear domain formation also drives transfer of proteins from disassembling transient nuclear bodies to its parental reassembling nuclear domain [7].…”

Section: Introductionmentioning

confidence: 99%

Identification of Genomic Loci Responsible for the Formation of Nuclear Domains Using Lampbrush Chromosomes

Krasikova

Kulikova

2019

ncRNA

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In the cell nuclei, various types of nuclear domains assemble as a result of transcriptional activity at specific chromosomal loci. Giant transcriptionally active lampbrush chromosomes, which form in oocyte nuclei of amphibians and birds enable the mapping of genomic sequences with high resolution and the visualization of individual transcription units. This makes avian and amphibian oocyte nuclei an advantageous model for studying locus-specific nuclear domains. We developed two strategies for identification and comprehensive analysis of the genomic loci involved in nuclear domain formation on lampbrush chromosomes. The first approach was based on the sequential FISH-mapping of BAC clones containing genomic DNA fragments with a known chromosomal position close to the locus of a nuclear domain. The second approach involved mechanical microdissection of the chromosomal region adjacent to the nuclear domain followed by the generation of FISH-probes and DNA sequencing. Furthermore, deciphering the DNA sequences from the dissected material by high throughput sequencing technologies and their mapping to the reference genome helps to identify the genomic region responsible for the formation of the nuclear domain. For those nuclear domains structured by nascent transcripts, identification of genomic loci of their formation is a crucial step in the identification of scaffold RNAs.

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RNA-dependent disassembly of nuclear bodies

Cited by 7 publications

References 57 publications

Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function

Lamin B2 Modulates Nucleolar Morphology, Dynamics, and Function

Live-Cell Imaging and Analysis of Nuclear Body Mobility

Identification of Genomic Loci Responsible for the Formation of Nuclear Domains Using Lampbrush Chromosomes

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