RNA degradation in human breast tissue after surgical removal: a time-course study

Ohashi, Yoko; Creek, Kim E.; Pirisi, Lucia; Kalus, Ram; Young, S. R.

doi:10.1016/j.yexmp.2004.05.005

Cited by 54 publications

(39 citation statements)

References 3 publications

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“…: 39 to 42 min, range 20 to 79) minutes after surgical removal. A pilot study performed on samples from 10 patients showed stable mRNA quantities of HER1-4 at 15, 30, and 60 minutes after surgical removal (unpublished data), and similar results have been published by Ohashi et al [27].…”

Section: Tissue Specimenssupporting

confidence: 81%

Expression of the Epidermal Growth Factor Receptors and Ligands in Paired Samples of Normal Breast Tissue, Primary Breast Carcinomas and Lymph Node Metastases

Brügmann¹,

Jensen²,

Garne³

et al. 2014

ABCR

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Purpose: In breast cancer, the EGF receptors host an increasing number of therapeutic targets and the interactive mechanisms of actions of the receptors and their ligands justify investigation of the EGF family as an entity. Experimental design: Paired tissue samples of normal breast tissue and primary breast carcinomas were examined in a prospective study of 163 patients. A third sample was obtained from the paired ipsilateral metastatic lymph node from 58 of these patients. The mRNA expression of four EGF receptors (HER1 -HER4) and 11 activating ligands was quantified with real-time RT-PCR. Results: Expression of HER2, HER3, and HER4 mRNA was upregulated in primary carcinomas compared to normal breast tissue while HER1 was downregulated. The mRNA expression of HER3 and HER4 differed between primary breast carcinomas and lymph node metastases whereas there was no difference in the expression of HER1 and HER2. The combination of low HER3 and low HER4 expression in the primary carcinoma was significantly more frequent in lymph node-negative patients as compared to lymph node positive patients. Distinct correlation patterns of the receptors and their corresponding activating ligands appeared in both normal breast tissue and in carcinomas, notably for the HER3 and HER4 receptors and their 3 specific ligands: HB-EGF, NRG2, and NRG4. Conclusion: HER2, HER3, and HER4 showed increased mRNA expression in carcinomas and were positively correlated to each other and to specific activating ligands. Furthermore, low HER3 and HER4 expression in the carcinomas correlated to the absence of lymph node metastases.

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Section: Tissue Specimenssupporting

confidence: 81%

Expression of the Epidermal Growth Factor Receptors and Ligands in Paired Samples of Normal Breast Tissue, Primary Breast Carcinomas and Lymph Node Metastases

Brügmann¹,

Jensen²,

Garne³

et al. 2014

ABCR

View full text Add to dashboard Cite

show abstract

“…In agreement with our observations, studies have been published that show intact RNA in tissue after incubation at room temperature over longer periods of time. 15,[20][21][22] However, these findings were only shown for specific tissue types and under certain specific experimental conditions.…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge, there is only one study comparing storage time dependency of gene expression levels using quantitative real-time PCR. 20 Fractions of breast cancer tissue were kept at room temperature for up to 3 h and gene expression of four housekeeping genes was analysed. Real-time PCR revealed stable expression levels over time.…”

Section: Discussionmentioning

confidence: 99%

Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

Micke

Ohshima

Tahmasebpoor

et al. 2006

Laboratory Investigation

203

145

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“…Several studies have been conducted concerning the stability of RNA in tissue ex vivo (22)(23)(24)(25)(26). These studies indicate that refrigeration is superior to room temperature storage and that the addition of RNase inhibitors may be useful as RNA preservatives.…”

mentioning

confidence: 99%

A Portrait of Tissue Phosphoprotein Stability in the Clinical Tissue Procurement Process

Espina

Edmiston

Heiby

et al. 2008

Molecular & Cellular Proteomics

191

194

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RNA degradation in human breast tissue after surgical removal: a time-course study

Cited by 54 publications

References 3 publications

Expression of the Epidermal Growth Factor Receptors and Ligands in Paired Samples of Normal Breast Tissue, Primary Breast Carcinomas and Lymph Node Metastases

Expression of the Epidermal Growth Factor Receptors and Ligands in Paired Samples of Normal Breast Tissue, Primary Breast Carcinomas and Lymph Node Metastases

Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens

A Portrait of Tissue Phosphoprotein Stability in the Clinical Tissue Procurement Process

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