RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells

Ishigaki, Yasuhito; Nakamura, Yuka; Tatsuno, Takanori; Hashimoto, Mitsumasa; Iwabuchi, Kuniyoshi; Tomosugi, Naohisa

doi:10.1007/s00418-013-1135-4

Cited by 25 publications

(28 citation statements)

References 45 publications

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“…Moreover, a putative RNA-binding protein called SZY-20 has been claimed to traffic to centrosomes and plays a critical role in limiting centrosome size in C. elegans [32]. Interestingly, the RNA-binding protein RBM8A, known to be required for mRNA metabolic processes such as splicing, mRNA export and nonsense-mediated mRNA decay, is localized to the centrosome and nuclei [33]. These data suggest that centrosomal and ciliary functions may be associated with mRNA transport and splicing, thus supporting our observations.…”

Section: Resultsmentioning

confidence: 99%

A network-based approach to dissect the cilia/centrosome complex interactome

et al. 2014

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BackgroundCilia are microtubule-based organelles protruding from almost all mammalian cells which, when dysfunctional, result in genetic disorders called “ciliopathies”. High-throughput studies have revealed that cilia are composed of thousands of proteins. However, despite many efforts, much remains to be determined regarding the biological functions of this increasingly important complex organelle.ResultsWe have derived an online tool, from a systematic network-based approach to dissect the cilia/centrosome complex interactome (CCCI). The tool integrates all current available data into a model which provides an “interaction” perspective on ciliary function. We generated a network of interactions between human proteins organized into functionally relevant “communities”, which can be defined as groups of genes that are both highly inter-connected and strongly co-expressed. We then combined sequence and co-expression data in order to identify the transcription factors responsible for regulating genes within their respective communities. Our analyses have discovered communities significantly specialized for delegating specific biological functions such as mRNA processing, protein translation, folding and degradation processes that had never been associated with ciliary proteins until now.ConclusionsCCCI will allow us to clarify the roles of previously unknown ciliary functions, elucidate the molecular mechanisms underlying ciliary-associated phenotypes, and apply our knowledge of the functional roles of relatively uncharacterized molecular entities to disease phenotypes and new clinical applications.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-658) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

A network-based approach to dissect the cilia/centrosome complex interactome

et al. 2014

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“…Our data indicate Magoh can influence microtubules indirectly, by modulating expression of key microtubule/mitosis regulatory proteins. However, Magoh may also directly influence microtubule organization; it localizes to the mitotic spindle (Ishigaki et al, 2013), was identified in proteomic studies as a mitotic spindle component (Bonner et al, 2011; Sauer, 2004), and physically associates with the microtubule-associated protein MAP1B (Tretyakova, 2005). It will be interesting to assess if Magoh’s RNA binding function is necessary for microtubule regulation and if so to identify its RNA cargos specifically at M phase.…”

Section: Discussionmentioning

confidence: 99%

Prolonged Mitosis of Neural Progenitors Alters Cell Fate in the Developing Brain

Pilaz

McMahon

Miller

et al. 2016

Neuron

169

203

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Summary Embryonic neocortical development depends upon balanced production of progenitors and neurons. Genetic mutations disrupting progenitor mitosis frequently impair neurogenesis, however the link between altered mitosis and cell fate remains poorly understood. Here we demonstrate that prolonged mitosis of radial glial progenitors directly alters neuronal fate specification and progeny viability. Live imaging of progenitors from a neurogenesis mutant, Magoh+/−, reveals mitotic delay significantly correlates with preferential production of neurons instead of progenitors, as well as apoptotic progeny. Independently, two pharmacological approaches reveal a causal relationship between mitotic delay and progeny fate. As mitotic duration increases, progenitors produce substantially more apoptotic or neurogenic progeny. We show apoptosis, but not differentiation, is p53-dependent, demonstrating these are distinct outcomes of mitotic delay. Together our findings reveal prolonged mitosis is sufficient to alter fates of radial glia progeny and define a new paradigm to understand how mitosis perturbations underlie brain size disorders such as microcephaly.

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“…HeLa and A549 are most popularly used and well documented cell lines, from our previous studies, we have clearly understood the biogenesis of RBM8A and Upf2 in HeLa and A549 (Ishigaki et al, ; Ishigaki et al, ; Ishigaki, Nakamura, Tatsuno, Ma, & Tomosugi, ; Tatsuno & Ishigaki, ; Tatsuno, Nakamura, Ma, Tomosugi & Ishigaki, ). We performed immunostaining for Y14 and Upf2 in an ultrathin section for TEM as shown in Figure .…”

Section: Resultsmentioning

confidence: 82%

“…Images provided by conventional TEM were shown in supporting information. Y14 is a member of the EJC and localize to perispeckles that is the place for mRNA processing (Ishigaki et al, 2013;Ishigaki et al, 2014). In the cells, the phosphorylation of Y14 is observed and its roles in mRNA processing have been proposed (Chuang, Peng, & Tarn, 2011;Ishigaki et al, 2015;Tatsuno & Ishigaki, 2018).…”

Section: Immunoelectron Microscopy With Postembeddingmentioning

confidence: 99%

Immuno‐detection of mRNA‐binding protein complex in human cells under transmission electron microscopy

Tatsuno

Nakamura

et al. 2019

Microscopy Res & Technique

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Transit from the nuclear complex to the cytoplasm through the nuclear pore complex permits modification of mRNA, including processing such as splicing, capping, and polyadenylation, etc. At each of these events, mRNA interacts with various proteins to form mRNA‐protein complex. Visualizing the mRNA is crucial for understanding the mechanisms underlying mRNA processing and elucidating its structure and recent advances in mRNA imaging allow detection of real‐time mRNA localization in living cells. However, these techniques revealed only the location of mRNA but cannot visualize the conformation of mRNA‐protein complex in cells. On the other hand, transmission electron microscopy has been used to visualize the structure of the Balbiani ring‐derived large mRNA, but their observations were limited to the insect cells. In this study, we visualized the structure of mRNA‐protein complex in human culture cells by using immuno‐electron microscopy. Through immuno‐detection, an mRNA exon junction binding complex Y14, and its binding protien Upf2, different gold particle patterns were imaged with transmission electron microscopy and analyzed. Characteristic linear and stacked particle orientation were observed. Across the nuclear membrane, only linear aggregation pattern was observed, whereas the stacked aggregation pattern was detected in the cytoplasm. Our method is able to visualize mRNA‐conformation and applicable to many cell types, including mammalian cells, where genes can easily be manipulated.

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RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells

Cited by 25 publications

References 45 publications

A network-based approach to dissect the cilia/centrosome complex interactome

A network-based approach to dissect the cilia/centrosome complex interactome

Prolonged Mitosis of Neural Progenitors Alters Cell Fate in the Developing Brain

Immuno‐detection of mRNA‐binding protein complex in human cells under transmission electron microscopy

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