RNA binding protein PUM1 promotes colon cancer cell proliferation and migration

Gor, Ravi; Sampath, Shruthi Sanjitha; Lazer, Lizha Mary; Ramalingam, Satish

doi:10.1016/j.ijbiomac.2021.01.154

Cited by 22 publications

(14 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the function of CSN-5 in FBF stabilization may be relevant for understanding cancer as hCSN5 is upregulated in many human cancers (Lee et al, 2011; Liu et al, 2020). Similarly, a number of cancers overexpress PUM1 and/or PUM2 (Guan et al, 2018; Gor et al, 2021; Shi et al, 2021; Smialek et al, 2021). This PUM protein overexpression in cancer might be borne out through its stabilization via hCSN5 and therefore reflect an unappreciated mechanism promoting cancer cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 inCaenorhabditis elegansgermline stem cells

Osterli

Ellenbecker

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

RNA-binding proteins FBF-1 and FBF-2 (FBFs) are required for germline stem cell maintenance in Caenorhabditis elegans and regulate the dynamics of progenitor cell proliferation and differentiation. The mechanisms controlling FBF protein levels remain unknown. We identified an interaction between both FBFs and CSN-5, a component of the COP9 (constitutive photomorphogenesis 9) signalosome. Here, we find that the MPN (Mpr1/Pad1 N-terminal) metalloprotease domain of CSN-5 interacts with the PUF RNA-binding domain of FBFs and the interaction is conserved for human homologs hPUM1 and hCSN5. This interaction likely takes place outside of the COP9 holoenzyme. csn-5 mutation results in destabilization of FBF proteins in germline stem cells leading to phenotypes consistent with a partial FBF loss of function. We propose that a COP9-independent role of CSN-5 in the PUF protein stability and function may be conserved across species, with implications for human stem cell biology and cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 inCaenorhabditis elegansgermline stem cells

Osterli

Ellenbecker

Wang

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…As a result, the tetrazolium salt was reduced to purple formazan crystals by the metabolic activity of the mitochondrial succinate dehydrogenase enzyme. Then dimethyl sulfoxide solution (DMSO, Sigma Aldrich, St. Louis, MO, USA) was used to dissolve the formazan crystals [ 34 , 35 ]. It helped to measure the optical density (OD) intensity of the purple colour solution which directly attributed to the concentration of live cells present [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Design and Development of Tantalum and Strontium Ion Doped Hydroxyapatite Composite Coating on Titanium Substrate: Structural and Human Osteoblast-like Cell Viability Studies

et al. 2023

View full text Add to dashboard Cite

In order to reduce the loosening of dental implants, surface modification with hydroxyapatite (HA) coating has shown promising results. Therefore, in this present study, the sol-gel technique has been employed to form a tantalum and strontium ion-doped hybrid HA layer coating onto the titanium (Ti)-alloy substrate. In this study, the surface modification was completed by using 3% tantalum pent oxide (Ta2O5), 3% strontium (Sr), and a combination of 1.5% Ta2O5 and 1.5% Sr as additives, along with HA gel by spin coating technique. These additives played a prominent role in producing a porous structure layer coating and further cell growth. The MG63 cell culture assay results indicated that due to the incorporation of strontium ions along with tantalum embedded in HA, cell proliferation increased significantly after a 48 h study. Therefore, the present results, including microstructure, crystal structure, binding energy, and cell proliferation, showed that the additives 1.5% Ta2O5 and 1.5% Sr embedded in HA on the Ti–substrate had an optimized porous coating structure, which will enhance bone in-growth in surface-modified Ti-implants. This material had a proper porous morphology with a roughness profile, which may be suitable for tissue in-growth between a surface-modified textured implant and bone interface and could be applicable for dental implants.

show abstract

“…Pumilio-1 (PUM1) is an RNA-binding pumilio protein with a dramatically increased expression level in various tumor tissues. PUM1 stimulates tumor cell proliferation, motility, and colony formation in colon cancer, and is required to regulate tumor spherification [ 114 ]. TPL therapy decreased PUM1 expression in PC, activating autophagy to enhance TRAIL sensitivity in tumor cells.…”

Section: Natural Products Overcome Autophagy-mediated Tumor Drug Resi...mentioning

confidence: 99%

Focusing on the Role of Natural Products in Overcoming Cancer Drug Resistance: An Autophagy-Based Perspective

Yao

Feng

et al. 2022

Biomolecules

View full text Add to dashboard Cite

Autophagy is a critical cellular adaptive response in tumor formation. Nutritional deficiency and hypoxia exacerbate autophagic flux in established malignancies, promoting tumor cell proliferation, migration, metastasis, and resistance to therapeutic interventions. Pro-survival autophagy inhibition may be a promising treatment option for advanced cancer. Furthermore, excessive or persistent autophagy is cytotoxic, resulting in tumor cell death. Targeted autophagy activation has also shown significant promise in the fight against tumor drug resistance. Several research groups have examined the ability of natural products (NPs) such as alkaloids, terpenoids, polyphenols, and anthraquinones to serve as autophagy inhibitors or activators. The data support the capacity of NPs that promote lethal autophagy or inhibit pro-survival autophagy from being employed against tumor drug resistance. This paper discusses the potential applications of NPs that regulate autophagy in the fight against tumor drug resistance, some limitations of the current studies, and future research needs and priorities.

show abstract

RNA binding protein PUM1 promotes colon cancer cell proliferation and migration

Cited by 22 publications

References 23 publications

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 inCaenorhabditis elegansgermline stem cells

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 inCaenorhabditis elegansgermline stem cells

Design and Development of Tantalum and Strontium Ion Doped Hydroxyapatite Composite Coating on Titanium Substrate: Structural and Human Osteoblast-like Cell Viability Studies

Focusing on the Role of Natural Products in Overcoming Cancer Drug Resistance: An Autophagy-Based Perspective

Contact Info

Product

Resources

About