RNA-based technologies for insect control in plant production

Liu, Shaoshuai; Jaouannet, Maëlle; Dempsey, D’Maris Amick; Imani, Jafargholi; Coustau, Christine; Kogel, Karl‐Heinz

doi:10.1016/j.biotechadv.2019.107463

Cited by 157 publications

(160 citation statements)

References 159 publications

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“…For example, the impairment of luminal uptake of dsRNA led to development of dsRNA-resistant western corn rootworm 46 . In insects, SID-1 and SID-1-like (SIL) proteins that encode transmembrane channel proteins, and receptor-mediated clathrindependent endocytosis 16,[47][48][49] were implicated in the cellular uptake of dsRNA. The dsRNA cell import has not been investigated in T. urticae.…”

Section: Discussionmentioning

confidence: 99%

Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

Bensoussan

Dixit

Tabara

et al. 2020

Preprint

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Comprehensive understanding of pleiotropic roles of RNAi machinery highlighted the conserved chromosomal functions of RNA interference. The consequences of the evolutionary variation in the core RNAi pathway genes are mostly unknown, but may lead to the species-specific functions associated with gene silencing. The two-spotted spider mite, Tetranychus urticae, is a major polyphagous chelicerate pest capable of feeding on over 1,100 plant species and developing resistance to pesticides used for its control. A well annotated genome, susceptibility to RNAi and economic importance, make T. urticae an excellent candidate for development of an RNAi protocol that enables high-throughput genetic screens and RNAibased pest control. Here, we show that the length of the exogenous dsRNA critically determines its processivity and ability to induce RNAi in vivo. A combination of the long dsRNAs and the use of dye to trace the ingestion of dsRNA enabled the identification of genes involved in membrane transport and 26S proteasome degradation as sensitive RNAi targets. Our data demonstrate that environmental RNAi can be an efficient reverse genetics and pest control tool in T. urticae. In addition, the species-specific properties together with the variation in the components of the RNAi machinery make T. urticae a potent experimental system to study the evolution of RNAi pathways.

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Section: Discussionmentioning

confidence: 99%

Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

Bensoussan

Dixit

Tabara

et al. 2020

Preprint

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“…Considerable variations in response to dsRNA has previously been reported in insects, including coleopteran, and has been linked to multiple contributing factors including dsRNA instability/degradation, delivery method, dsRNA uptake efficiency, etc. [ 60 , 61 ]. In addition to a variation in RNAi efficiency ( i .…”

Section: Resultsmentioning

confidence: 99%

Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor

et al. 2020

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In a number of species, individuals exposed to pathogens can mount an immune response and transmit this immunological experience to their offspring, thereby protecting them against persistent threats. Such vertical transfer of immunity, named trans-generational immune priming (TGIP), has been described in both vertebrates and invertebrates. Although increasingly studied during the last decade, the mechanisms underlying TGIP in invertebrates are still elusive, especially those protecting the earliest offspring life stage, i . e . the embryo developing in the egg. In the present study, we combined different proteomic and transcriptomic approaches to determine whether mothers transfer a “signal” (such as fragments of infecting bacteria), mRNA and/or protein/peptide effectors to protect their eggs against two natural bacterial pathogens, namely the Gram-positive Bacillus thuringiensis and the Gram-negative Serratia entomophila . By taking the mealworm beetle Tenebrio molitor as a biological model, our results suggest that eggs are mainly protected by an active direct transfer of a restricted number of immune proteins and of antimicrobial peptides. In contrast, the present data do not support the involvement of mRNA transfer while the transmission of a “signal”, if it happens, is marginal and only occurs within 24h after maternal exposure to bacteria. This work exemplifies how combining global approaches helps to disentangle the different scenarios of a complex trait, providing a comprehensive characterization of TGIP mechanisms in T . molitor . It also paves the way for future alike studies focusing on TGIP in a wide range of invertebrates and vertebrates to identify additional candidates that could be specific to TGIP and to investigate whether the TGIP mechanisms found herein are specific or common to all insect species.

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“…Compared with chemical pesticides, employing an RNAibased strategy is an environmentally friendlier way to control insect pests, since it can be designed specifically to control the target insect pest without impacting beneficial insects or other animals such as pollinators or pest parasitoids (Scott et al, 2013;Yu et al, 2016;Cooper et al, 2019;Liu et al, 2019). For example, species-specific dsRNAs for Snf7 or V-ATPaseE killed the targeted insects without affecting non-targeted species (Whyard et al, 2009;Bachman et al, 2013;Liu et al, 2019). In our study, phylogenetic analysis showed that the NlATG1 protein of BPH closely resembles the ATG1 protein (ATV91623.1) of R. dorsalis.…”

Section: Discussionmentioning

confidence: 99%

“…For example, piercing-sucking insects feed on phloem sap while ingesting very little plant tissue, so foliar application of dsRNA by spraying is not likely to trigger an effective RNAi response in them. Microinjection is an effective strategy for delivering dsRNA into many orders of insects, including BPH Zhu et al, 2017;Waris et al, 2018;Ge et al, 2019), but this approach is not a feasible strategy for achieving widespread crop protection in the field (Huvenne and Smagghe, 2010;Scott et al, 2013;Liu et al, 2019). Recently, Li et al (2015) suggested that soaking the root system in dsRNA could be used as a dsRNA-delivery strategy during crop irrigation, which is also suitable for BPH control.…”

Section: Discussionmentioning

confidence: 99%

NlATG1 Gene Participates in Regulating Autophagy and Fission of Mitochondria in the Brown Planthopper, Nilaparvata lugens

Hao

et al. 2020

Front. Physiol.

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Autophagy plays multiple roles in regulating various physiological processes in cells. However, we currently lack a systematic analysis of autophagy and the autophagyrelated gene 1 ATG1 in the brown planthopper (BPH, Nilaparvata lugens), one of the most destructive of the insect pests of rice. In this study, the full-length cDNA of an autophagy-related gene, NlATG1, was cloned from BPH. Real-time qPCR (RT-qPCR) revealed that this NlATG1 gene was expressed differently across developmental stages, at higher levels in nymphs but lower levels in adults. RNA interference with dsNlATG1 significantly decreased the mRNA level of the target gene to 14.6% at day 4 compared with that of the dsGFP control group. The survival of the dsNlATG1-treated group decreased significantly from day 4 onward, dropping to 48.3% on day 8. Examination using transmission electron microscopy (TEM) showed that epithelial cells of the BPH's midgut in the dsNlATG1-treated group had less autophagic vacuoles than did the dsGFP control, and knockdown of NlATG1 clearly inhibited the starvation-induced autophagy response in this insect. RNA interference of NlATG1 upregulated the NlFis1 gene involved in mitochondrial fission, leading to reductions in mitochondrial width and area. Furthermore, knockdown of NlATG1 also decreased the ATP content and accumulation of glycogen. Together, these results demonstrate that the NlATG1 gene participates in regulating autophagy and fission of mitochondria in the brown planthopper, making it a potentially promising target for pest control given its key role in autophagy, including maintaining the normal structure and function of mitochondria.

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RNA-based technologies for insect control in plant production

Cited by 157 publications

References 159 publications

Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response

Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor

NlATG1 Gene Participates in Regulating Autophagy and Fission of Mitochondria in the Brown Planthopper, Nilaparvata lugens

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