RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin

Janardhanan, Pavithra; Mello, Charlene M.; Singh, Bal Ram; Lou, Jianlong; Marks, James D.; Cai, Shuowei

doi:10.1016/j.talanta.2013.09.012

Cited by 26 publications

(22 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An electrochemical aptasensor with a 5-minute detection time and 40 pg/mL limit of detection has been developed [54,55]. A surface plasmon resonance-based RNA aptasensor with a detection limit of 22.5 ng/mL in diluted human serum has also recently been reported [56]. Aptamer-based sensors for the detection of BoNT have potential applications in both food safety and bioterrorism.…”

Section: Aptamers For Neurotoxin Detectionmentioning

confidence: 99%

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

McConnell

Holahan

DeRosa

2014

Nucleic Acid Therapeutics

View full text Add to dashboard Cite

Oligonucleotide aptamers are short, synthetic, single-stranded DNA or RNA able to recognize and bind to a multitude of targets ranging from small molecules to cells. Aptamers have emerged as valuable tools for fundamental research, clinical diagnosis, and therapy. Due to their small size, strong target affinity, lack of immunogenicity, and ease of chemical modification, aptamers are an attractive alternative to other molecular recognition elements, such as antibodies. Although it is a challenging environment, the central nervous system and related molecular targets present an exciting potential area for aptamer research. Aptamers hold promise for targeted drug delivery, diagnostics, and therapeutics. Here we review recent advances in aptamer research for neurotransmitter and neurotoxin targets, demyelinating disease and spinal cord injury, cerebrovascular disorders, pathologies related to protein aggregation (Alzheimer's, Parkinson's, and prions), brain cancer (glioblastomas and gliomas), and regulation of receptor function. Challenges and limitations posed by the blood brain barrier are described. Future perspectives for the application of aptamers to the central nervous system are also discussed.

show abstract

Section: Aptamers For Neurotoxin Detectionmentioning

confidence: 99%

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

McConnell

Holahan

DeRosa

2014

Nucleic Acid Therapeutics

View full text Add to dashboard Cite

show abstract

“…The recognition limit range of this method is 5Á8-23Á4 ng ml À1 . This sensor is robust, has a shelf-life of at least 30 days and can be reused for at least 400 tests (Janardhanan et al 2013). Bruno et al developed aptamers against a botulinum neurotoxin (BoNT) type A light chain (Table 1).…”

Section: Botulinum Neurotoxinsmentioning

confidence: 99%

Current advances in aptamer-assisted technologies for detecting bacterial and fungal toxins

et al. 2018

View full text Add to dashboard Cite

SummaryInfectious diseases are among the common leading causes of morbidity and mortality worldwide. Associated with the emergence of new infectious diseases, the increasing number of antimicrobial-resistant isolates presents a serious threat to public health and hospitalized patients. A microbial pathogen may elicit several host responses and use a variety of mechanisms to evade host defences. These methods and mechanisms include capsule, lipopolysaccharides or cell wall components, adhesions and toxins. Toxins inhibit phagocytosis, cause septic shock and host cell damages by binding to host surface receptors and invasion. Bacterial and fungal pathogens are able to apply many different toxin-dependent mechanisms to disturb signalling pathways and the structural integrity of host cells for establishing and maintaining infections Initial techniques for analysis of bacterial toxins were based on in vivo or in vitro assessments. There is a permanent demand for appropriate detection methods which are affordable, practical, careful, rapid, sensitive, efficient and economical. Aptamers are DNA or RNA oligonucleotides that are selected by systematic evolution of ligands using exponential enrichment (SELEX) methods and can be applied in diagnostic applications. This review provides an overview of aptamer-based methods as a novel approach for detecting toxins in bacterial and fungal pathogens.

show abstract

“…For detection of botulinum neurotoxin, Wei and Ho [81] developed an aptamer-based electrochemical sensor to achieve sensitive detection, with a limit of detection at 40 pg/mL. Janardhanan et al [82] designed a SPR-based RNA aptasensor for rapid detection of type A botulinum neurotoxin. The detection limits of the aptasensor in carrot juice and fat-free milk were 20.3 and 23.4 ng/mL, respectively.…”

Section: Biotoxinsmentioning

confidence: 99%

Aptamer-Based Technology for Food Analysis

Liu

Zhang

2014

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

show abstract

RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin

Cited by 26 publications

References 32 publications

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

Aptamers as Promising Molecular Recognition Elements for Diagnostics and Therapeutics in the Central Nervous System

Current advances in aptamer-assisted technologies for detecting bacterial and fungal toxins

Aptamer-Based Technology for Food Analysis

Contact Info

Product

Resources

About