RLP, a bacteriophage of the family Podoviridae, rescues mice from bacteremia caused by multi-drug-resistant Pseudomonas aeruginosa

Alvi, Iqbal Ahmad; Asif, Muhammad; Tabassum, Rabia; Aslam, Rehan; Abbas, Zaigham; Rehman, Sami Ur

doi:10.1007/s00705-020-04601-x

Cited by 34 publications

(35 citation statements)

References 45 publications

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“…High effectiveness of phage therapy in the treatment of experimental sepsis induced by multidrug resistant P. aeruginosa was also confirmed by Alvi et al [10]. Phage-treated bacteremic mice had a survival rate of almost 100%, and no viable pathogen could be detected at 96 h post inoculation.…”

Section: Phage Therapy Of Experimentally Induced Sepsismentioning

confidence: 67%

Sepsis, Phages, and COVID-19

2020

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Phage therapy has emerged as a potential novel treatment of sepsis for which no decisive progress has been achieved thus far. Obviously, phages can help eradicate local bacterial infection and bacteremia that may occur in a syndrome. For example, phages may be helpful in correcting excessive inflammatory responses and aberrant immunity that occur in sepsis. Data from animal studies strongly suggest that phages may indeed be an efficient means of therapy for experimentally induced sepsis. In recent years, a number of reports have appeared describing the successful treatment of patients with sepsis. Moreover, novel data on the anti-viral potential of phages may be interpreted as suggesting that phages could be used as an adjunct therapy in severe COVID-19. Thus, clinical trials assessing the value of phage therapy in sepsis, including viral sepsis, are urgently needed.

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Section: Phage Therapy Of Experimentally Induced Sepsismentioning

confidence: 67%

Sepsis, Phages, and COVID-19

2020

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“…The dried coated copper grid was flooded with 15 µL of phospho-tungstic acid (pH 7.0). The sample was inserted in the FEI T20 TEM after drying, and 200 KV accelerating voltage was used to obtain the image (26,27).…”

Section: Morphological Study Of Ebpmentioning

confidence: 99%

Characterization of a lytic EBP bacteriophage with large size genome against Enterobacter cloacae

Asif

Naseem

Alvi

et al. 2021

APMIS

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Enterobacter cloacae (E. cloacae) is an emerging nosocomial pathogen that had acquired antibiotic resistance against multiple classes of antibiotics. The current study was aimed to isolate and characterize lytic bacteriophage against E. cloacae. The bacteriophage EBP was isolated from a sewage water sample using E. cloacae as a host strain by double-layer agar technique. EBP was found stabile at a wide range of temperatures (25, 37, 60, and 80°C) and pH (5, 6, 7, 8, and 9) with antibacterial activity up to 24 h of infection. The latent period of EBP was 20 min with a burst size of 252 phages per cell. It showed a narrow host range and infected 12/21 (57%) isolates of E. cloacae tested. It has helical symmetry with a head size of 105 and 120 nm long tail with contractile sheath. The EBP has 179.1 kb long doublestranded DNA genome with 44.8% GC content. Majority of identified ORFs (187/281) were encoding putative proteins with unknown function. Necessary replication enzymes, structural proteins, and lytic enzymes were detected in the genome of EBP. Phylogenetic analysis revealed that EBP closely resembles with Coronobacter phage vB_CsaM_IeN, vB_CsaM_IeE, vB_CsaM_IeB, and Citrobacter phage Margaery. Based on electron microscopy and molecular characterization, EBP was classified as a Myoviridae phage.

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“…Bacteriophage host range was determined by standard spot assay and e ciency of plating as described earlier [16] A collection of 32 MRSA strains, 8 MSSA, 4 S epidermidis strains and some species of gram negative organisms (E. coli, Klebsiella pneumoniae, Serratia marsecence, Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae) were used to measure the host range and EOP of bacteriophage TSP.…”

Section: Determination Of Tsp Host Rangementioning

confidence: 99%

“…Storage stability of TSP phage was determined by incubating phage lysate at different temperatures (4, 25, -20 and -80ºC) for 1 month as described earlier [17]. Bacteriophage stability at different temperatures (25,37,45,50, and 60°C) and a wide range of pH values (4, 5, 6, 7, 8, 9, and 10) was done according to the previously described procedure [16]. The survival ability of bacteriophage was determined by the double-layer agar technique.…”

Section: Determination Of Bacteriophage Stability At Different Tempermentioning

confidence: 99%

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

Tabassum¹,

Alvi²,

Asif³

et al. 2021

Preprint

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Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing nosocomial pathogen that causes a large number of diseases in healthcare and community settings. The MRSA causes infections in different tissues of immunocompromised individuals leading to increased morbidity and mortality. It possess various virulence mechanisms to show resistance against to a lot of beta-lactam antibiotics. To tackle this emerging issue of MRSA, there is an urgent need of antibiotic alternatives and utilizing lytic bacteriophages is one of the best promising therapeutic approach. In the present study, a lytic bacteriophage TSP was isolated from hospital wastewater against MRSA. Its morphology, physiology, host specificity, burst size and lytic spectrum were determined and complete genome sequence was analyzed. TSP phage efficiently inhibit bacterial growth for up to 12 hours. TSP phage showed broad lytic spectrum against clinical isolates of MRSA (78%) and MSSA (37%). It showed stability at varying temperatures (25ºC, 37ºC) and pH (5–9), while its maximum storage stability was observed at 4ºC. It had short latent period (20min) and high burst size (103 PFU/ infected cell). TSP genome sequence and restriction analysis revealed that its genome is linear having 17,987 bp in length with an average GC content of 29.7%. The TSP genome showed 98% similarity to S aureus phages SCH1, SCH11 and vB SauP-436A1. According to comparative genomic analysis and phylogenetic tree analysis, TSP phage can be considered as a member of genus “P68viruses”. The strong lytic activity, broad host range and short latent period along with absence of any lysogenic and toxic genes make TSP a very good candidate for phage therapy against MRSA infections if prove safe during in vivo studies.

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RLP, a bacteriophage of the family Podoviridae, rescues mice from bacteremia caused by multi-drug-resistant Pseudomonas aeruginosa

Cited by 34 publications

References 45 publications

Sepsis, Phages, and COVID-19

Sepsis, Phages, and COVID-19

Characterization of a lytic EBP bacteriophage with large size genome against Enterobacter cloacae

TSP, a virulent Podovirus can control the growth of Staphylococcus aureus till 12 hours

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