Rituximab modulates T- and B-lymphocyte subsets and urinary CD80 excretion in patients with steroid-dependent nephrotic syndrome

Bhatia, Divya; Sinha, Aditi; Hari, Pankaj; Sopory, Shailaja; Saini, Sharanjot; Puraswani, Mamta; Saini, Himanshi; Mitra, Dipendra Kumar; Bagga, Arvind

doi:10.1038/s41390-018-0088-7

Cited by 52 publications

(44 citation statements)

References 41 publications

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“…Kidney tissue was minced into 1-2 mm 3 pieces and digested using collagenase type I (2 mg/mL; Thermo Fisher Scientific). The single-cell suspensions obtained after digestion were subsequently incubated with ACK lysing buffer (Thermo Fisher Scientific) for the lysis of RBCs, followed by isolation of renal mononuclear cells (RMNCs) by Ficoll-Hypaque density gradient centrifugation method (50). The macrophages from mouse RMNCs were sorted using anti-CD115 biotin-conjugated antibody and anti-biotin microbeads (Miltenyi Biotec) using MACS cell separation protocol for CD115 positive selection (Supplemental Figure 4A and ref.…”

Section: Methodsmentioning

confidence: 99%

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Mitophagy-dependent macrophage reprogramming protects against kidney fibrosis

et al. 2019

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Section: Methodsmentioning

confidence: 99%

“…PBMC isolation. PBMCs were isolated from whole blood using the Ficoll-Paque (GE Healthcare) density gradient centrifugation method as previously described (50). Blood samples were first diluted in PBS in 1:1 and layered on the surface of Ficoll-Paque and centrifuged at 670 g for 20 minutes at 20°C with no breaks.…”

Section: Methodsmentioning

confidence: 99%

Mitophagy-dependent macrophage reprogramming protects against kidney fibrosis

et al. 2019

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“…Selective extracorporeal immunoadsorption induces the remission of nephrotic syndrome relapses, suggesting that the factor responsible for proteinuria could be an immunoglobulin or could bind to immunoglobulins 90 . B cells can also act as antigen-presenting cells and their depletion may also modify T cell function in patients with steroid-dependent nephrotic syndrome (SDNS) 91 . In a subset of patients, hyposialylated IgM on the surface to the consideration of these forms as T cell-mediated diseases 77 .…”

Section: Syndromic Disordersmentioning

confidence: 99%

Podocytopathies

Kopp

Anders

Suszták

et al. 2020

Nat Rev Dis Primers

298

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Podocytopathies are kidney diseases in which direct or indirect podocyte injury drives proteinuria or nephrotic syndrome. In children and young adults, genetic variants in >50 podocyte-expressed genes, syndromal non-podocyte-specific genes and phenocopies with other underlying genetic abnormalities cause podocytopathies associated with steroid-resistant nephrotic syndrome or severe proteinuria. A variety of genetic variants likely contribute to disease development. Among genes with non-Mendelian inheritance, variants in APOL1 have the largest effect size. In addition to genetic variants, environmental triggers such as immune-related, infection-related, toxic and haemodynamic factors and obesity are also important causes of podocyte injury and frequently combine to cause various degrees of proteinuria in children and adults. Typical manifestations on kidney biopsy are minimal change lesions and focal segmental glomerulosclerosis lesions. Standard treatment for primary podocytopathies manifesting with focal segmental glomerulosclerosis lesions includes glucocorticoids and other immunosuppressive drugs; individuals not responding with a resolution of proteinuria have a poor renal prognosis. Renin-angiotensin system antagonists help to control proteinuria and slow the progression of fibrosis. Symptomatic management may include the use of diuretics, statins, infection prophylaxis and anticoagulation. This Primer discusses a shift in paradigm from patient stratification based on kidney biopsy findings towards personalized management based on clinical, morphological and genetic data as well as pathophysiological understanding.

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“…SRCP-35-2A, USA). Figures and analyses were performed using the FACSDIVA 6.1 software (BD Bioscience, San Joes, CA, USA) [14,15].…”

Section: For Lymphocytes Subsetsmentioning

confidence: 99%

B Lymphocytes Are Predictors of Insulin Resistance in Women with Gestational Diabetes Mellitus

Zhuang

Zhang

et al. 2019

EMIDDT

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Backgroud: The present study aimed to investigate the association between immune cells and gestational diabetes mellitus (GDM) and identify a reasonable predictor of insulin resistance in women with GDM. Objective: The clinical and biochemical characteristics of 124 women with GDM and 168 healthy pregnant women were compared. Methods: The percentage of immune cells in the blood of the subjects was analyzed by flow cytometry. Pearson’s correlation analysis revealed the correlation between the percentage of B lymphocytes and insulin resistance. A cutoff point was determined for the percentage of B lymphocytes, based on insulin resistance, using receiver operating characteristic (ROC) curves. Results: Compared to the healthy pregnant women, the percentages of B lymphocytes and IgA produced by B-cells were significantly different in women with GDM. The percentage of B lymphocytes was positively related to insulin resistance.The number of 14.05% of B lymphocytes was an optimal cutoff point that predicted the insulin resistance in women with GDM. Conclusion: The percentage of B lymphocytes was positively associated with insulin resistance, and hence, might serve as an appropriate predictor of insulin resistance in women with GDM.

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Rituximab modulates T- and B-lymphocyte subsets and urinary CD80 excretion in patients with steroid-dependent nephrotic syndrome

Abstract: Rituximab-induced sustained remission and B-cell depletion was associated with reduced numbers of Th17 and Th2 lymphocytes, and increased Treg cells; these changes reversed during relapses. Recovery of B cells and memory B cells predicted the occurrence of a relapse.

Cited by 52 publications

References 41 publications

Mitophagy-dependent macrophage reprogramming protects against kidney fibrosis

Mitophagy-dependent macrophage reprogramming protects against kidney fibrosis

Podocytopathies

B Lymphocytes Are Predictors of Insulin Resistance in Women with Gestational Diabetes Mellitus

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