Risk of cytomegalovirus transmission by cryopreserved semen: a study of 635 semen samples from 231 donors

Bresson, Jean Luc

doi:10.1093/humrep/deg362

Cited by 36 publications

(25 citation statements)

References 38 publications

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“…As might be expected, CMV DNA was undetectable in all of our HIV-infected participants in blood plasma, despite frequent detection of very high levels of CMV replication in semen (median of 4.8 log 10 copies CMV DNA/ml seminal plasma among positive samples). Such compartmentalized replication of CMV in the genital tract, first described in 1972 (45) and subsequently confirmed by others (11,46,60), probably has consequences for CMV transmission (11,43), impacts localized immune responses, and interacts with other sexually transmitted viruses, like HIV-1 (66).…”

Section: Discussionmentioning

confidence: 75%

Associations between Virologic and Immunologic Dynamics in Blood and in the Male Genital Tract

Gianella

Strain

Rought

et al. 2012

J Virol

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To determine the influence of asymptomatic genital viral infections on the cellular components of semen and blood, we evaluated the associations between the numbers and activation statuses of CD4 ؉ and CD8 ؉ T lymphocytes in both compartments and the seminal levels of cytomegalovirus (CMV), herpes simplex virus (HSV), and human immunodeficiency virus 1 (HIV). Paired blood and semen samples were collected from 36 HIV-infected antiretroviral-naïve individuals and from 40 HIV-uninfected participants. We performed multiparameter flow cytometry analysis (CD45, CD45RA, CD3, CD4, CD8, and CD38) of seminal and blood cellular components and measured HIV RNA and CMV and HSV DNA levels in seminal and blood plasma by real-time PCR. Compared to HIV-uninfected participants, in the seminal compartment HIV-infected participants had higher levels of CMV (P < 0.05), higher numbers of total CD3 ؉ (P < 0.01) and CD8 ؉ subset (P < 0.01) T lymphocytes, and higher CD4 ؉ and CD8 ؉ T lymphocyte activation (RA-CD38 ؉ ) (P < 0.01). Seminal CMV levels positively correlated with absolute numbers of CD4 ؉ and CD8 ؉ T cells in semen (P < 0.05) and with the activation status of CD4 ؉ T cells in semen and in blood (P < 0.01). HIV levels in semen (P < 0.05) and blood (P < 0.01) were positively associated with T-cell activation in blood. Activation of CD8 ؉ T cells in blood remained an independent predictor of HIV levels in semen in multivariate analysis. The virologic milieu in the male genital tract strongly influences the recruitment and activation of immune cells in semen and may also modulate T-cell immune activation in blood. These factors likely influence replication dynamics, sexual transmission risk, and disease outcomes for all three viruses.

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Section: Discussionmentioning

confidence: 75%

Associations between Virologic and Immunologic Dynamics in Blood and in the Male Genital Tract

Gianella

Strain

Rought

et al. 2012

J Virol

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“…A number of studies have documented approximately 5-10% of men testing positive for HCMV by semen analysis, with no evidence of either immune compromise or genital infection [41,42]. HCMV adheres to the surface of sperm cells [43] and interferes with the invasiveness of the trophoblast within the maternal-fetal interface [44] by selectively targeting HLA-G for degradation [45] and reducing the expression of molecules that promote placentation [46].…”

Section: Explanation Of Resultsmentioning

confidence: 99%

The impact of donor insemination on the risk of preeclampsia: a systematic review and meta-analysis

González-Comadran

Avila

Tascón

et al. 2014

European Journal of Obstetrics & Gynecology and Reproductiv

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“…HCMV DNA was detected in 8.7% of semen samples from healthy infertility patients, the highest among all sexually transmitted viruses [7], and in approximately 3% of cryopreserved semen samples for sperm donations [8]. The percentage increases to as high as 65% in a subpopulation of HIV infected, antiretroviral therapy-naïve men [55].…”

Section: Introductionmentioning

confidence: 99%

The salivary glands as a privileged site of cytomegalovirus immune evasion and persistence

Campbell

Cavanaugh

Slater

2008

Med Microbiol Immunol

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The salivary glands (SG) provide a haven for persistent cytomegalovirus replication, and in this regard are a privileged site of virus immune evasion. The murine cytomegalovirus (MCMV) model has provided insight into the immunological environment of the SG and the unqiue virus-host relationship of this organ. In response to MCMV infection, a robust T cell-mediated immune response is elicited, comprised predominantly of CD8+ T cells that phenotypically and functionally appear activated. However, they fail to clear virus by an unknown evasion mechanism that is independent of inhibitory NKG2A-or Programmed Death 1-mediated signaling. Virus is eventually eliminated from the SG by eVector CD4+ T cells expressing antiviral cytokines. However, this mechanism is severely dampened by high levels of the immunosuppressive cytokine IL-10, selectively expressed by SG CD4+ T cells.

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Risk of cytomegalovirus transmission by cryopreserved semen: a study of 635 semen samples from 231 donors

Abstract: The best strategy to prevent potential CMV risk is to test donors for CMV IgG and IgM antibody at the outset and after a 6 month period of quarantine and to reject initially IgM seropositive donors or donors who seroconvert during the quarantine period.

Cited by 36 publications

References 38 publications

Associations between Virologic and Immunologic Dynamics in Blood and in the Male Genital Tract

Associations between Virologic and Immunologic Dynamics in Blood and in the Male Genital Tract

The impact of donor insemination on the risk of preeclampsia: a systematic review and meta-analysis

The salivary glands as a privileged site of cytomegalovirus immune evasion and persistence

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