Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand

Arunvipas, P.; Inpankaew, Tawin; Jittapalapong, Sathaporn

doi:10.1007/s11250-011-0048-2

Cited by 21 publications

(13 citation statements)

References 23 publications

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“…In this present study, most farms had at least one dog in nearby environment. Hence, the presence of dogs on a farm has been a potential risk to provide the increasing chance of horizontal transmission (Jittapalapong et al 2008;Arunvipas et al 2012b). …”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Inpankaew¹,

Jittapalapong

Mitchell

et al. 2014

Acta Parasitologica

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Neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of neosporosis, recognized as a major cause of bovine abortion around the world. Thailand is a developing agricultural country located in Southeast Asia. Livestock developments particularly in dairy cows of this country have been hampered by low productivity including milk and slow growth rate due to the impact of many pathogens including N. caninum. Currently, there is no effective method for control of neosporosis since there is less information regarding current status of infections. The objective of this study was to investigate the seroprevalence of neosporosis in dairy cows of the northern part of Thailand. During 2006-2007, the sera of 642 cows from 42 small farm holders with the top three highest consensus of dairy farms in the northern provinces, such as Chiang Rai, Chiang Mai and Lumpang were collected and performed tests. Antibodies to N. caninum were assayed by enzyme-linked immunosorbent assay (ELISA) with recombinant N. caninum surface antigen 1 (NcSAG1) and indirect fluorescent antibody test (IFAT). The overall prevalence of N. caninum infection in this study was 46.9% (301/642) by ELISA and 34.3% (220/642) by IFAT.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Inpankaew¹,

Jittapalapong

Mitchell

et al. 2014

Acta Parasitologica

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“…The same methods cELISA and/or IFAT were used also in Brazil; N. caninum antibodies were found in 25% of 400 (Bresciani et al 2007) and in none of 70 (Coelho et al 2011) cats, respectively. In Thailand, 36 tested cats were negative for N. caninum antibodies in both IFAT and cELISA (Arunvipas et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Neospora caninum in cats from the Czech Republic

Sedlák

Bártová

Machačová

2014

Acta Parasitologica

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Sera of 414 cats coming from different parts of the Czech Republic were tested for N. caninum antibodies. Sera samples were collected during years [2002][2003][2004][2005][2006][2007][2008][2009][2010][2011]. N. caninum antibodies were detected by a commercial competitive-inhibition enzyme-linked immunosorbent assay (cELISA) with cut off ≥30% inhibition. Samples positive in cELISA were confirmed by an indirect fluorescence antibody test (IFAT); titre ≥50 was considered positive. In total, 137 (33%) cats reacted positively in cELISA; N. caninum antibodies in IFAT were detected in 16 (3.86%) cats with titres 50 and 100. In 6 cats, positive for N. caninum antibodies, T. gondii antibodies were also detected by IFAT. It is the first report of N. caninum antibodies in domestic cats from the Czech Republic and third report in Europe.

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“…Dogs, especially farm dogs, are an important factor in the spread of N. caninum infection. They can become infected by ingesting tissues such as livers, lungs, and brains, as well as aborted foetuses and placental material from infected cattle [ 32 ]. Following infection, N. caninum oocysts are shed in their faeces which contaminate water, pasture, and the environment of cattle farms.…”

Section: Discussionmentioning

confidence: 99%

“…In Thailand, the seroprevalence of bovine neosporosis in dairy cattle farms ranges from 3 to 47% depending on the type of serological test and cut-off level used, and the areas surveyed [ 32 , 42 – 44 ]. Few studies have reported the presence of N. caninum in dogs, although 1.2% of dogs near farms in Nakhon Pathom Province were shown to be N. caninum- positive by competitive ELISA [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…Few studies have reported the presence of N. caninum in dogs, although 1.2% of dogs near farms in Nakhon Pathom Province were shown to be N. caninum- positive by competitive ELISA [ 42 ]. In another study carried out in Western Thailand, 7% of dogs were found to be positive for the same test, and 4.4% by an indirect fluorescent antibody test [ 32 ]. Although serological antibody testing is the most common method used for dogs that have the past or recent contact with the parasite, it is not correlated with the shedding of oocysts [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

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Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis

et al. 2017

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BackgroundDogs are the definitive hosts of Neospora caninum and play an important role in the transmission of the parasite. Despite the high sensitivity of existing molecular tools such as quantitative real-time PCR (qPCR), these techniques are not suitable for use in many countries because of equipment costs and difficulties in implementing them for field diagnostics. Therefore, we developed a simplified technique, loop-mediated isothermal amplification (LAMP), for the rapid visual detection of N. caninum.MethodsLAMP specificity was evaluated using a panel containing DNA from a range of different organisms. Sensitivity was evaluated by preparing 10-fold serial dilutions of N. caninum tachyzoites and comparing the results with those obtained using qPCR. Assessment of the LAMP results was determined by recognition of a colour change after amplification. The usefulness of the LAMP assay in the field was tested on 396 blood and 115 faecal samples from dogs, and one placenta from a heifer collected in Lopburi, Nakhon Pathom, Sa Kaeo, and Ratchaburi provinces, Thailand.ResultsSpecificity of the LAMP technique was shown by its inability to amplify DNA from non-target pathogens or healthy dogs. The detection limit was the equivalent of one genome for both LAMP and qPCR. LAMP and qPCR detected positive N. caninum infection in 15 of 396 (3.8%) blood samples; LAMP detected 9/115 (7.8%) positive faecal samples, while qPCR detected 5/115 (4.3%) positive faecal samples. The placental tissue was shown to be positive by both techniques. Agreement between LAMP and qPCR was perfect in blood samples (kappa value, 1.00) and substantial in faecal samples (kappa value, 0.697).ConclusionsThis is the first known LAMP assay developed for the amplification of N. caninum. The technique effectively and rapidly detected the parasite with high sensitivity and specificity and was cost-effective. This assay could be used in the field to confirm the diagnosis of canine or bovine neosporosis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2330-2) contains supplementary material, which is available to authorized users.

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Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand

Cited by 21 publications

References 23 publications

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

Seroprevalence of Neospora caninum in cats from the Czech Republic

Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis

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