Risk factors for equine trypanosomosis and hematological analysis of horses in Paraguay

Yamazaki, Ai; Suganuma, Keisuke; Kayano, Mitunori; Acosta, Tomás J.; Saitoh, Tomoko; Valinotti, Maria Fátima Rodríguez; Sanchez, Antonio Rodríguez; Inoue, Noboru

doi:10.1016/j.actatropica.2022.106543

Cited by 3 publications

(1 citation statement)

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“…The focus of most of the published literature on tabanid species from Brazil and the rest of South America is their taxonomy [4][5][6][7], ecology [8,9] and behavior [10,11] in different biomes, and molecular tools were not used for identification or bioprospecting in these studies. More specifically, few studies have addressed tabanids in southern Brazil [12][13][14][15][16][17], and only one reported the molecular detection of a strain of a trypanosomatid, Trypanosoma kaiowa, which is associated with the crocodilian clade of Trypanosoma [18].…”

Section: Introductionmentioning

confidence: 99%

First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America

et al. 2023

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Background Trypanosoma evansi infects a large number of wild and domestic animals and causes a spoliative disease known as surra. It is mechanically transmitted, mainly by biting flies of the genera Tabanus and Stomoxys. The detection of T. evansi DNA in the feeding apparatus of Dichelacera alcicornis and Dichelacera januarii from South America is reported, to the best of our knowledge, for the first time. Methods Tabanids were collected weekly from February 2018 to February 2019 from two sites. The feeding apparatus was removed and DNA extraction, polymerase chain reaction and sequencing were performed. Results A 205-base pair fragment of the variant surface protein RoTat 1.2 gene, confirmed by DNA sequencing, was amplified from the feeding apparatus of D. alcicornis and D. januarii. Conclusions This is, to the best of our knowledge, the first record of T. evansi DNA in South American tabanids. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America

et al. 2023

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Parasitological and molecular detection of Trypanosoma evansi in a dog from Tocache, San Martin, Peru

Villena,

Puicón,

López

et al. 2023

Veterinary Parasitology: Regional Studies and Reports

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European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test

Hébert,

Froger,

Madeline

et al. 2023

Veterinary Sciences

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Dourine is a sexually transmitted parasitic disease affecting equids. Its causative agent is referred to as Trypanosoma equiperdum and the prescribed serodiagnosis method is the complement fixation test (CFT). In the context of our European Reference Laboratory mandate for equine diseases (excluding African horse sickness), we organised dourine CFT inter-laboratory proficiency tests (ILPTs) in 2015, 2018 and 2022 to evaluate the performance of the European Union network of National Reference Laboratories (NRLs) for dourine. ILPT panels were composed of horse sera with or without antibodies against Trypanosoma spp. originating from non-infected, immunised or experimentally infected horses. Twenty-two NRLs participated in at least one of the three sessions. In 2015, 2018 and 2022, the percentage of laboratories obtaining 100% of the expected results was 57, 90 and 80, respectively. These dourine CFT ILPTs showed the benefits of standardising the method’s detection limit and underlined the constant need to evaluate NRLs to improve the network’s performance. These results also argue in favour of the need for a representative bio-bank to improve the representativeness of ILPT samples and to allow the adoption of alternative serological methods for international surveillance of dourine.

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Risk factors for equine trypanosomosis and hematological analysis of horses in Paraguay

Cited by 3 publications

References 35 publications

First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America

First record of Trypanosoma evansi DNA in Dichelacera alcicornis and Dichelacera januarii (Diptera: Tabanidae) flies in South America

Parasitological and molecular detection of Trypanosoma evansi in a dog from Tocache, San Martin, Peru

European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test

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