“…In consistent with our findings, the studies conducted by Derikvand et al (2018) and Mahmoudvand et al, (2018) have reported a significant correlation between teeth brushing and the prevalence of E. gingivalis and T. tenax ( Derikvand , 2018 ; Mahmoudvand et al, 2018 ) . Previous studies have reported people living in rural regions poor due to lack of attention to oral health, more frequency of tooth loss and gum diseases was observed ( Zander et al, 2013 ; Chen, 2018 ). Similarly, our findings showed that there was a significant correlation between living in rural regions and the frequency of E. gingivalis and T. tenax; this considerable prevalence in rural communities may be due to some factors such as the low compliance with health standards, lower educational level, more contact between people, and lack of access to health services ( Coughlin et al, 2019 ).…”