“…The degree of abrasion of demineralized teeth, in turn, depends on the applied force, 31 filament stiffness and end-rounding of the toothbrush, 32 abrasive properties of the dentifrice, 32 brushing frequency and number of applied strokes, and demineralization extent of the enamel. 33 Because patients suffering from erosive tooth wear are usually recommended to use toothbrushes with relatively soft filaments, 34,35 soft brushes (Trisa Ultra®) with a load of 200 g 36,37 and 20 strikes were applied in the present study for the abrasive treatment. No dentifrice or slurry was used in experiment 1 to avoid side remineralization effects by the components of dentifrice (Ca 2þ , F − , phosphates, etc).…”